DOI: 10.1007/s11745-000-0580-4. Do glycerolipids display lateral heterogeneity in the thylakoid membrane?

Do glycerolipids display lateral heterogeneity in the thylakoid membrane?

10.1007/s11745-000-0580-4

Crossref journal-article

Wiley

Lipids (311)

Abstract

AbstractThe lateral heterogeneity of lipids in the thylakoid membrane has been questioned for over 20 yrs. It is generally believed that glycerolipids are asymmetrically distributed within the plane of the membrane. In the present investigation, we isolated several thylakoid membrane domains by using sonication followed by separation in an aqueous dextran‐polyethylene glycol two‐phase system. This technique, which avoids detergent treatments, allowed us to obtain stroma and grana lamellae vesicles as well as grana central core and grana margin vesicles from thylakoids. The relative distribution of the four lipid classes, i.e., monogalactosyldiacylglycerol, digalactosyldiacylglycerol, sulfoquinovosyldiacylglycerol, and phosphatidylglycerol, was found to be statistically identical in all four thylakoid fractions and in whole thylakoids. Similarly, the relative amount of fatty acids in each individual lipid and the eight main phosphatidylglycerol molecular species was identical in all thylakoid membrane fractions tested as well as in the intact thylakoid membrane. Based on presently available procedures for obtaining thylakoid subfractions that are unable to discriminate microdomains within the membrane, it is concluded that glycerolipids are evenly distributed within the plane of the thylakoid membrane. These data are discussed in terms of “bulk” and “specific” lipids.

Bibliography

DuchÃªne, S., & Siegenthaler, P. (2000). Do glycerolipids display lateral heterogeneity in the thylakoid membrane? Lipids, 35(7), 739â744. Portico.

Authors 2

Sylvie Duchêne (first)
Paul‐André Siegenthaler (additional)

References 40 Referenced 45

{'key': 'e_1_2_2_2_1', 'first-page': '119', 'volume-title': 'Lipids in Photosynthesis: Structure, Function and Genetics', 'author': 'Siegenthaler P.A.', 'year': '1998'} / Lipids in Photosynthesis: Structure, Function and Genetics by Siegenthaler P.A. (1998)
{'key': 'e_1_2_2_3_1', 'first-page': '293', 'article-title': 'Occurrence and Function of Phosphatidylglycerol Containing Δ3‐trans‐Hexadecenoic Acid in Photosynthetic Lamellae', 'volume': '21', 'author': 'Dubacq J.P.', 'year': '1983', 'journal-title': 'Physiol. Vég.'} / Physiol. Vég. / Occurrence and Function of Phosphatidylglycerol Containing Δ3‐trans‐Hexadecenoic Acid in Photosynthetic Lamellae by Dubacq J.P. (1983)
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{'key': 'e_1_2_2_6_1', 'first-page': '291', 'article-title': 'Rôle Structural des lipides dans les membranes des chloroplastes de Blé', 'volume': '10', 'author': 'Costes C.', 'year': '1972', 'journal-title': 'Physiol. Vég.'} / Physiol. Vég. / Rôle Structural des lipides dans les membranes des chloroplastes de Blé by Costes C. (1972)
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Dates

Type	When
Created	18 years, 9 months ago (Nov. 15, 2006, 8:49 a.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 7:28 p.m.)
Indexed	2 months, 1 week ago (June 26, 2025, 12:10 a.m.)
Issued	25 years, 2 months ago (July 1, 2000)
Published	25 years, 2 months ago (July 1, 2000)
Published Online	25 years, 2 months ago (July 1, 2000)
Published Print	25 years, 2 months ago (July 1, 2000)

Funders 0

None

BibTeX

@article{Duch_ne_2000, title={Do glycerolipids display lateral heterogeneity in the thylakoid membrane?}, volume={35}, ISSN={1558-9307}, url={http://dx.doi.org/10.1007/s11745-000-0580-4}, DOI={10.1007/s11745-000-0580-4}, number={7}, journal={Lipids}, publisher={Wiley}, author={Duchêne, Sylvie and Siegenthaler, Paul‐André}, year={2000}, month=jul, pages={739–744} }

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