DOI: 10.1007/s10005-001-0021-3. In Situ Environmental Cell–Transmission Electron Microscopy Study of Microbial Reduction of Chromium(VI) Using Electron Energy Loss Spectroscopy

In Situ Environmental Cell–Transmission Electron Microscopy Study of Microbial Reduction of Chromium(VI) Using Electron Energy Loss Spectroscopy

10.1007/s10005-001-0021-3

Crossref journal-article

Oxford University Press (OUP)

Microscopy and Microanalysis (286)

Abstract

AbstractReduction of Cr(VI) by the bacterium, Shewanella oneidensis (previously classified Shewanella putrefaciens strain MR-1), was studied by absorption spectrophotometry and in situ, environmental cell–transmission electron microscopy (EC-TEM) coupled with electron energy loss spectroscopy (EELS). Bacteria from rinsed cultures were placed directly in the environmental cell of the transmission electron microscope and examined under 100 Torr pressure. Bright field EC-TEM images show two distinct populations of S. oneidensis in incubated cultures containing Cr(VI)O42−: those that exhibit low image contrast and heavily precipitateencrusted cells exhibiting high image contrast. Several EELS techniques were applied to determine the oxidation state of Cr associated with encrusted cells. The encrusted cells are shown to contain a reduced form of Cr in oxidation state +3 or lower. These results demonstrate the capability to determine the chemistry and valence state of reduction products associated with unfixed, hydrated bacteria in an environmental cell transmission electron microscope.

Bibliography

Daulton, T. L., Little, B. J., Lowe, K., & Jones-Meehan, J. (2001). In Situ Environmental CellâTransmission Electron Microscopy Study of Microbial Reduction of Chromium(VI) Using Electron Energy Loss Spectroscopy. Microscopy and Microanalysis, 7(6), 470â485.

Authors 4

Tyrone L. Daulton (first)
Brenda J. Little (additional)
Kristine Lowe (additional)
Joanne Jones-Meehan (additional)

References 0 Referenced 96

None

Dates

Type	When
Created	5 years, 2 months ago (June 22, 2020, 4:49 p.m.)
Deposited	2 years, 6 months ago (Feb. 14, 2023, 6:31 a.m.)
Indexed	2 months, 3 weeks ago (May 30, 2025, 2:23 p.m.)
Issued	23 years, 9 months ago (Nov. 1, 2001)
Published	23 years, 9 months ago (Nov. 1, 2001)
Published Online	23 years, 6 months ago (Feb. 2, 2002)
Published Print	23 years, 9 months ago (Nov. 1, 2001)

Funders 0

None

BibTeX

@article{Daulton_2001, title={In Situ Environmental Cell–Transmission Electron Microscopy Study of Microbial Reduction of Chromium(VI) Using Electron Energy Loss Spectroscopy}, volume={7}, ISSN={1435-8115}, url={http://dx.doi.org/10.1007/s10005-001-0021-3}, DOI={10.1007/s10005-001-0021-3}, number={6}, journal={Microscopy and Microanalysis}, publisher={Oxford University Press (OUP)}, author={Daulton, Tyrone L. and Little, Brenda J. and Lowe, Kristine and Jones-Meehan, Joanne}, year={2001}, month=nov, pages={470–485} }

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