DOI: 10.1007/bf02663969. Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography

Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography

10.1007/bf02663969

Crossref journal-article

Wiley

Journal of the American Oil Chemists' Society (311)

Abstract

AbstractA quantitative chromatographic procedure for the fractionation of complex lipid mixtures is described. The method utilizes diethylaminoethyl (DEAE) cellulose column chromatography followed by thin layer chromatography (TLC). Spots produced in TLC are charred with sulfuric acid‐potassium dichromate and heat and are then measured by quantitative densitometry. Results obtained with beef brain and beef heart mitochondrial lipids are presented, and the close correspondence between column isolation procedures and the new procedure is demonstrated. Methods utilizing only column chromatography, column chromatography and TLC, and one‐ and two‐dimensional TLC without column chromatography are compared.

Bibliography

Rouser, G., Galli, C., Lieber, E., Blank, M. L., & Privett, O. S. (1964). Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography. Journal of the American Oil Chemistsâ Society, 41(12), 836â840. Portico.

Authors 5

George Rouser (first)
Claudio Galli (additional)
Ellen Lieber (additional)
M. L. Blank (additional)
O. S. Privett (additional)

References 13 Referenced 189

10.1111/j.1748-1716.1952.tb00862.x
10.3181/00379727-83-20422
10.1016/S0021-9258(19)77323-2
10.1042/bj0600353
10.1007/BF02633600
{'key': 'e_1_2_1_7_1', 'first-page': '425', 'volume': '40', 'author': 'Rouser G.', 'year': '1963', 'journal-title': 'Ibid.'} / Ibid. by Rouser G. (1963)
{'key': 'e_1_2_1_8_1', 'first-page': '70', 'volume': '40', 'author': 'Privett O. S.', 'year': '1963', 'journal-title': 'Ibid.'} / Ibid. by Privett O. S. (1963)
{'key': 'e_1_2_1_9_1', 'first-page': '371', 'volume': '41', 'author': 'Blank M. L.', 'year': '1964', 'journal-title': 'Ibid.'} / Ibid. by Blank M. L. (1964)
Fleischer S. B.Fleischer G.RouserandP. P.Nair in preparation.
10.1007/BF02633111
{'key': 'e_1_2_1_12_1', 'first-page': '565', 'volume': '38', 'author': 'Rouser G.', 'year': '1961', 'journal-title': 'Ibid.'} / Ibid. by Rouser G. (1961)
Das M. G.RouserandC.Galli in preparation.
Rouser G. C.Galli G.KritchevskyandD.Heller JAOCS in press.

Dates

Type	When
Created	18 years, 1 month ago (July 20, 2007, 12:34 a.m.)
Deposited	1 year, 10 months ago (Sept. 27, 2023, 2:50 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:26 a.m.)
Issued	60 years, 8 months ago (Dec. 1, 1964)
Published	60 years, 8 months ago (Dec. 1, 1964)
Published Online	60 years, 8 months ago (Dec. 1, 1964)
Published Print	60 years, 8 months ago (Dec. 1, 1964)

Funders 0

None

BibTeX

@article{Rouser_1964, title={Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography}, volume={41}, ISSN={1558-9331}, url={http://dx.doi.org/10.1007/bf02663969}, DOI={10.1007/bf02663969}, number={12}, journal={Journal of the American Oil Chemists’ Society}, publisher={Wiley}, author={Rouser, George and Galli, Claudio and Lieber, Ellen and Blank, M. L. and Privett, O. S.}, year={1964}, month=dec, pages={836–840} }

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