DOI: 10.1007/bf02540130. Precise ultramicro determination of steroid hormones by combined TLC and GLC analysis

Precise ultramicro determination of steroid hormones by combined TLC and GLC analysis

10.1007/bf02540130

Crossref journal-article

Wiley

Journal of the American Oil Chemists' Society (311)

Abstract

AbstractThe migration of steroid hormones on TLC plates is predictable on the basis of the number of double bonds and carbonyl and hydroxyl groups they contain. Developed plates can therefore be divided into zones or bands corresponding to well‐defined classes of steroids. Steroids can be eluted from the adsorbent quantitatively and without contamination. The eluate from each zone contains a mixture which is much simpler than the original one.The conversion of OH‐containing steroids to trimethylsilyl ether (TMS) derivatives is quantitative; it is complete after 3 hr by the described technic. All TMS derivatives are very stable in CS2 solution. Whether or not they contain OH groups, steroids other than corticosteroids are stable under the present GLC conditions after treatment with TMS reagents. With amts of injected sample increasing from 0 to 0.2 Ɓg, a small apparent increase in specific response is observed with some steroids. This effect is reproducible. The use of calibration curves obtained with an internal standard (5ॅ‐pregnane) leads to accurate analyses of mixtures with injections containing 25 nanograms (0.025 Ɓg) of individual steroid. Values obtained from single trials fall 95% of the time within 2% of the mean.Peak elution time of each compound is accurately predictable on the basis of number, position, and stereoconfiguration of functional groups.An integrated TLC‐GLC method of analysis based on these findings is outlined.

Bibliography

Vandenheuvel, F. A., Hinderks, G. J., & Nixon, J. C. (1965). Precise ultramicro determination of steroid hormones by combined TLC and GLC analysis. Journal of the American Oil Chemistsâ Society, 42(4), 283â290. Portico.

Authors 3

F. A. Vandenheuvel (first)
G. J. Hinderks (additional)
J. C. Nixon (additional)

References 10 Referenced 37

10.1021/ac60216a016
{'key': 'e_1_2_1_3_1', 'first-page': '1186', 'volume': '35', 'author': 'Vandenheuvel F. A.', 'year': '1963', 'journal-title': 'Ibid.'} / Ibid. by Vandenheuvel F. A. (1963)
{'key': 'e_1_2_1_4_1', 'first-page': '811', 'volume-title': 'The Chromatography of Steroids', 'author': 'Bush I. E.', 'year': '1961'} / The Chromatography of Steroids by Bush I. E. (1961)
{'key': 'e_1_2_1_5_1', 'first-page': '112', 'volume-title': 'Gas Chromatography', 'author': 'Keulemans A. I. M.', 'year': '1957'} / Gas Chromatography by Keulemans A. I. M. (1957)
{'key': 'e_1_2_1_6_1', 'first-page': '104', 'volume-title': 'Methods of Biochemical Analysis', 'author': 'Horning', 'year': '1963'} / Methods of Biochemical Analysis by Horning (1963)
10.1016/0006-3002(61)90426-7
10.1021/ac60197a048
10.1016/S0022-2275(20)40462-6
10.1038/194833a0
10.1016/0006-3002(62)90299-8

Dates

Type	When
Created	18 years, 7 months ago (Jan. 4, 2007, 11:59 p.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 4:09 a.m.)
Indexed	3 months, 1 week ago (May 26, 2025, 2:46 a.m.)
Issued	60 years, 5 months ago (April 1, 1965)
Published	60 years, 5 months ago (April 1, 1965)
Published Online	60 years, 5 months ago (April 1, 1965)
Published Print	60 years, 5 months ago (April 1, 1965)

Funders 0

None

BibTeX

@article{Vandenheuvel_1965, title={Precise ultramicro determination of steroid hormones by combined TLC and GLC analysis}, volume={42}, ISSN={1558-9331}, url={http://dx.doi.org/10.1007/bf02540130}, DOI={10.1007/bf02540130}, number={4}, journal={Journal of the American Oil Chemists’ Society}, publisher={Wiley}, author={Vandenheuvel, F. A. and Hinderks, G. J. and Nixon, J. C.}, year={1965}, month=apr, pages={283–290} }

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