DOI: 10.1007/bf02537245. An improved method for the separation of molecular species of cerebrosides

An improved method for the separation of molecular species of cerebrosides

10.1007/bf02537245

Crossref journal-article

Wiley

Lipids (311)

Abstract

AbstractA method employing reversed‐phase high performance liquid chromatography (HPLC) has been developed for the analysis of molecular species of underivatized cerebrosides. By using a high carbon (20%) C18‐silica bead column, monohexosylceramides isolated from bovine brain and Japanese quail intestine could each be separated into 42 and 56 peaks, respectively, and could subsequently be recovered. Under our analytical conditions, underivatized cerebrosides with 24∶1 and 22∶0 fatty acids were completely separated by single step HPLC. Each of the molecular species was then analyzed by high performance thin layer chromatography and gas chromatography‐mass spectrometry (or fast atom bombardment mass spectrometry). This analytical method permits the complete identification of molecular species of glycosphingo‐lipids.

Bibliography

Hirabayashi, Y., Hamaoka, A., Matsumoto, M., & Nishimura, K. (1986). An improved method for the separation of molecular species of cerebrosides. Lipids, 21(11), 710â714. Portico.

Authors 4

Yoshio Hirabayashi (first)
Akira Hamaoka (additional)
Makoto Matsumoto (additional)
Kenji Nishimura (additional)

References 13 Referenced 33

10.1016/0005-2760(79)90149-8
10.1111/j.1471-4159.1980.tb11199.x
10.1016/0005-2760(83)90085-1
{'key': 'e_1_2_1_5_1', 'first-page': '23', 'volume-title': 'New Vistas in Glycolipid Research', 'author': 'Handa S.', 'year': '1982'} / New Vistas in Glycolipid Research by Handa S. (1982)
10.1016/S0022-2275(20)37775-0
10.1016/S0022-2275(20)40579-6
{'key': 'e_1_2_1_8_1', 'first-page': '679', 'volume': '22', 'author': 'Smith M.', 'year': '1981', 'journal-title': 'J. Lipid Res.'} / J. Lipid Res. by Smith M. (1981)
10.1016/S0022-2275(20)37384-3
10.1016/0005-2760(84)90127-9
{'key': 'e_1_2_1_11_1', 'first-page': '25', 'volume': '24', 'author': 'Ito T.', 'year': '1982', 'journal-title': 'Proc. Jpn. Conf. Biochem. Lipids'} / Proc. Jpn. Conf. Biochem. Lipids by Ito T. (1982)
10.1093/oxfordjournals.jbchem.a133894
10.1016/0005-2760(78)90107-8
10.1093/oxfordjournals.jbchem.a135391

Dates

Type	When
Created	18 years, 8 months ago (Nov. 29, 2006, 12:49 a.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 12:12 p.m.)
Indexed	1 year, 10 months ago (Oct. 21, 2023, 12:08 p.m.)
Issued	38 years, 9 months ago (Nov. 1, 1986)
Published	38 years, 9 months ago (Nov. 1, 1986)
Published Online	38 years, 9 months ago (Nov. 1, 1986)
Published Print	38 years, 9 months ago (Nov. 1, 1986)

Funders 0

None

BibTeX

@article{Hirabayashi_1986, title={An improved method for the separation of molecular species of cerebrosides}, volume={21}, ISSN={1558-9307}, url={http://dx.doi.org/10.1007/bf02537245}, DOI={10.1007/bf02537245}, number={11}, journal={Lipids}, publisher={Wiley}, author={Hirabayashi, Yoshio and Hamaoka, Akira and Matsumoto, Makoto and Nishimura, Kenji}, year={1986}, month=nov, pages={710–714} }

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