DOI: 10.1007/bf02536479. An extended method for separating and quantitating molecular species of phospholipids

An extended method for separating and quantitating molecular species of phospholipids

10.1007/bf02536479

Crossref journal-article

Wiley

Lipids (311)

Abstract

AbstractAN improved and extended method for separating and quantitating molecular species of four phospholipid classes is presented. Crude lipid extract is first separated into phospholipid classes on a silica column. Each phospholipid class is then separated into molecular species without derivatization using high‐performance liquid chromatography on columns packed with octadecyl silica. Quantitation of individual species is achieved by measuring absorbance at 205 nm. Factors for converting absorbancies to mol fractions have been determined. Quantitation by absorbance at 205 nm agrees well with quantitation by gas chromatography which is preferred to quantitation by phosphate analysis. One hundred phospholipid species have been identified. A table of relative retention times of molecular species is provided. Examples of quantitative analyses of species composition are presented.

Bibliography

Wiley, M. G., Przetakiewicz, M., Takahashi, M., & Lowenstein, J. M. (1992). An extended method for separating and quantitating molecular species of phospholipids. Lipids, 27(4), 295â301. Portico.

Authors 4

Margaret G. Wiley (first)
Marek Przetakiewicz (additional)
Mareyuki Takahashi (additional)
John M. Lowenstein (additional)

References 25 Referenced 24

10.1016/S0022-2275(20)37381-8
10.1016/S0022-2275(20)38188-8
10.1007/BF02537245
10.1016/S0022-2275(20)37909-8
10.1016/S0021-9673(01)92744-X
10.1016/0003-9861(86)90727-7
10.1093/oxfordjournals.jbchem.a135285
10.1007/BF02535701
10.1042/bj2640909
10.1016/S0378-4347(00)82817-0
10.1016/0005-2760(77)90234-X
10.1139/o84-049
10.1016/S0022-2275(20)38208-0
10.1016/S0021-9258(18)64849-5
10.1016/S0021-9673(01)92081-3
10.1007/BF02534829
10.1016/S0076-6879(81)72004-4
10.1016/0003-2697(74)90259-0
10.1021/bi00328a014
{'key': 'e_1_2_2_21_1', 'first-page': '154', 'volume': '52', 'author': 'Takahashi M.', 'year': '1992', 'journal-title': 'Cancer Res.'} / Cancer Res. by Takahashi M. (1992)
10.1016/S0022-2275(20)37763-4
10.1016/B978-0-12-654150-2.50016-6
10.1016/S0022-2275(20)37909-8
10.1016/S0378-4347(00)84625-3
10.1007/BF02534776

Dates

Type	When
Created	18 years, 8 months ago (Nov. 29, 2006, 9:19 a.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 4:41 p.m.)
Indexed	1 year, 9 months ago (Oct. 27, 2023, 10:40 a.m.)
Issued	33 years, 4 months ago (April 1, 1992)
Published	33 years, 4 months ago (April 1, 1992)
Published Online	33 years, 4 months ago (April 1, 1992)
Published Print	33 years, 4 months ago (April 1, 1992)

Funders 0

None

BibTeX

@article{Wiley_1992, title={An extended method for separating and quantitating molecular species of phospholipids}, volume={27}, ISSN={1558-9307}, url={http://dx.doi.org/10.1007/bf02536479}, DOI={10.1007/bf02536479}, number={4}, journal={Lipids}, publisher={Wiley}, author={Wiley, Margaret G. and Przetakiewicz, Marek and Takahashi, Mareyuki and Lowenstein, John M.}, year={1992}, month=apr, pages={295–301} }

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