DOI: 10.1007/bf02535993. The role of arginines in stabilizing the active open‐lid conformation of<i>Rhizomucor miehei</i> lipase

The role of arginines in stabilizing the active open‐lid conformation ofRhizomucor miehei lipase

10.1007/bf02535993

Crossref journal-article

Wiley

Lipids (311)

Abstract

AbstractMolecular dynamics simulations for the lid covering the active site ofRhizomucor miehei lipase [EC 3.1.1.3] postulated that, among other interactions, Arg86 in the lid stabilized the open‐lid conformation of the protein by multiple hydrogen bonding to the protein surface. Chemical modification of arginine residues inR. miehei lipase with 1,2‐cyclohexanedione or phenylglyoxal resulted in residual activities in the hydrolysis of tributyrin of 66 and 46%, respectively. Tryptic maps of native and phenylglyoxal‐reactedR. miehei lipase showed that Arg86 was the residue modified most, when the lipase was inhibited to the greatest extent. Guanidine, a structural analog to an arginine side chain, inhibited both the natibe enzyme and the arginine‐modified enzymes, resulting in residual activities of 26% as compared to the native enzyme. The inhibition was not an effect of enzyme denaturation. The native enzyme was also inhibited by 1‐ethylguanidine, benzamidine and urea, but to a lesser degree than by guanidine. Lipases fromHumicola lanuginosa and porcine pancreas in 100 mM guanidine showed residual activities of 88 and 70%, respectively. The lipases fromCandida antarctica, C. rugosa, Pseudomonas cepacia andP. fluoresences were not inhibited by guanidine. The inhibition ofR. miehei lipase by structural analogs of the arginine side chain and after chemical modification of arginine residues suggest a role of an arginine residue in stabilizing the active open‐lid conformation of the enzyme.

Bibliography

Holmquist, M., Norin, M., & Hult, K. (1993). The role of arginines in stabilizing the active openâlid conformation ofRhizomucor miehei lipase. Lipids, 28(8), 721â726. Portico.

Authors 3

Mats Holmquist (first)
Martin Norin (additional)
Karl Hult (additional)

References 22 Referenced 32

10.1111/j.1748-1716.1945.tb03084.x
10.1016/0006-3002(58)90097-0
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10.1021/bi00120a034
10.1016/S0021-9258(19)41933-9
10.1016/S0021-9258(18)94475-3
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{'key': 'e_1_2_2_14_1', 'first-page': '41', 'volume-title': 'The Analysis of Peptides and Proteins by Mass Spectrometry', 'author': 'Jardine I.', 'year': '1988'} / The Analysis of Peptides and Proteins by Mass Spectrometry by Jardine I. (1988)
{'key': 'e_1_2_2_15_1', 'first-page': '111', 'volume-title': 'The Third Dimension in Organic Chemistry', 'author': 'Bassindale A.', 'year': '1984'} / The Third Dimension in Organic Chemistry by Bassindale A. (1984)
{'key': 'e_1_2_2_16_1', 'first-page': '7279', 'volume': '104', 'author': 'Chen C.‐S.', 'year': '1982', 'journal-title': 'J. Am. Chem. Soc.'} / J. Am. Chem. Soc. by Chen C.‐S. (1982)
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Brady L. Brzozowski A.M. Derewenda Z.S. Dodson E. Dodson G. Tolley S. Turkenburg J.P. Christiansen L. Huge‐Jensen B. Norskov L. Thim L. andMenge U.(1990)Data Commision of the International Union of Chrystallography Bonn/Cambridge/Chester.
Norin M. Olsen O. Svendsen A. Edholm O. andHult K.(1993)Prot. Eng. in press.
10.1093/oxfordjournals.jbchem.a131472
{'key': 'e_1_2_2_23_1', 'first-page': '216', 'volume': '305', 'author': 'Boel E.', 'year': '1988', 'journal-title': 'Eur. Pat. Appl.'} / Eur. Pat. Appl. by Boel E. (1988)

Dates

Type	When
Created	18 years, 9 months ago (Nov. 29, 2006, 12:59 p.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 11 p.m.)
Indexed	1 year ago (Aug. 12, 2024, 8:35 a.m.)
Issued	32 years, 1 month ago (Aug. 1, 1993)
Published	32 years, 1 month ago (Aug. 1, 1993)
Published Online	32 years, 1 month ago (Aug. 1, 1993)
Published Print	32 years, 1 month ago (Aug. 1, 1993)

Funders 0

None

BibTeX

@article{Holmquist_1993, title={The role of arginines in stabilizing the active open‐lid conformation ofRhizomucor miehei lipase}, volume={28}, ISSN={1558-9307}, url={http://dx.doi.org/10.1007/bf02535993}, DOI={10.1007/bf02535993}, number={8}, journal={Lipids}, publisher={Wiley}, author={Holmquist, Mats and Norin, Martin and Hult, Karl}, year={1993}, month=aug, pages={721–726} }

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