DOI: 10.1007/bf01696153. Evaluation of pepsin treatment for electron microscopic RNA in situ hybridization on ultra-thin cryosections of cultured cells

Evaluation of pepsin treatment for electron microscopic RNA in situ hybridization on ultra-thin cryosections of cultured cells

10.1007/bf01696153

Crossref journal-article

Springer Science and Business Media LLC

Histochemistry and Cell Biology (297)

Bibliography

Macville, M. V. E., Dorp, A. G. M., Dirks, R. W., Fransen, J. A. M., & Raap, A. K. (1996). Evaluation of pepsin treatment for electron microscopic RNA in situ hybridization on ultra-thin cryosections of cultured cells. Histochemistry and Cell Biology, 105(2), 139â145.

Authors 5

Merryn V. E. Macville (first)
Annette G. M. Dorp (additional)
Roeland W. Dirks (additional)
Jack A. M. Fransen (additional)
Anton K. Raap (additional)

References 30 Referenced 5

Artvinli S (1975) Biochemical aspects of aldehyde fixation and a new formaldehyde fixative. Histochem J 7:435 (10.1007/BF01003881) / Histochem J by S Artvinli (1975)
Bauman JGJ, Bentvelzen P (1988) Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization. Cytometry 9:517–524 (10.1002/cyto.990090602) / Cytometry by JGJ Bauman (1988)
Boom R, Geelen JL, Sol CJ, Raap AK, Minnaar RP, Klaver BP, Van der Noordaa J (1986) Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate early gene products by protein synthesis inhibition. J Virol 58:851–859 (10.1128/JVI.58.3.851-859.1986) / J Virol by R Boom (1986)
Dirks RW, Van Dorp AGM, Van Minnen J, Fransen JAM, Van der Ploeg M, Raap AK (1992) Electron microscopic detection of RNA sequences by non-radioactive in situ hybridization in the molluskLymnaea stagnalis. J Histochem Cytochem 40: 1647–1657 (10.1177/40.11.1431053) / J Histochem Cytochem by RW Dirks (1992)
Dulhunty AF, Junankar PR, Stanhope C (1993) Immunogold labeling of calcium ATPase in sarcoplasmic reticulum of skeletal muscle: use of 1-nm, 5-nm, and 10-nm gold. J Histochem Cytochem 41:1459–1466 (10.1177/41.10.8245405) / J Histochem Cytochem by AF Dulhunty (1993)
Egger D, Troxler M, Bienz K (1994) Light and electron microscopic in situ hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry. J Histochem Cytochem 42:815–822 (10.1177/42.6.8189042) / J Histochem Cytochem by D Egger (1994)
Erickson JM, Rushford CL, Dorney DJ, Wilson GN, Schmickel RD (1981) Structure and variation of human ribosomal DNA: molecular analysis of cloned fragments. Gene 16:1–19 (10.1016/0378-1119(81)90055-X) / Gene by JM Erickson (1981)
Gilerovitch HG, Bishop GA, King JS, Burry RW (1995) The use of electron microscopic immunocytochemistry with silver-enhanced 1,4-nm gold particles to localize GAD in the cercbellar nuclei. J Histochem Cytochem 43:337–343 (10.1177/43.3.7868863) / J Histochem Cytochem by HG Gilerovitch (1995)
Huang S, Deerinck TJ, Ellisman MH, Spector DL (1994) In vivo analysis of the stability and transport of nuclear poly(A)+ RNA. J Cell Biol 126:877–899 (10.1083/jcb.126.4.877) / J Cell Biol by S Huang (1994)
Humbel BM, Biegelmann E (1992) A preparation protocol for post-embedding immunoelectron microscopy ofDictyostelium discoideum cells with monoclonal antibodies. Scan Microsc 6: 817–825 / Scan Microsc by BM Humbel (1992)
Le Guellec D, Trembleau A, Pechoux C, Gossard F, Morel G (1992) Ultrastructural non-radioactive in situ hybridization of GH mRNA in rat pituitary gland: pre-embedding vs ultra-thin frozen sections vs post-embedding. J Histochem Cytochem 40:979–986 (10.1177/40.7.1607645) / J Histochem Cytochem by D Guellec Le (1992)
Leduc EH, Bernhard W (1962) Water-soluble embedding media for ultrastructural cytochemistry: digestion with nucleinases and proteinases. Symp Int Soc Cell Biol 1:21–44 / Symp Int Soc Cell Biol by EH Leduc (1962)
Leduc EH, Holt SJ (1965) Hydroxypropyl methacrylate, a new water-miscible embedding medium for electron microscopy. J Cell Biol 26:137–155 (10.1083/jcb.26.1.137) / J Cell Biol by EH Leduc (1965)
Lewis PR, Knight DP (1992) General cytochemical methods. In: Glauert AM (ed) Cytochemical staining methods for electron microscopy. (Practical methods in electron microscopy, vol 14). Elsevier. Amsterdam, pp 79–145 / Cytochemical staining methods for electron microscopy / Practical methods in electron microscopy by PR Lewis (1992)
Lloyd RV, Jin L, Song J (1990) Ultrastructural localization of prolactin and chromogranin B messenger ribonucleic acids with biotinylated oligonucleotide probes in cultured pituitary cells. Lab Invest 63:413–419 / Lab Invest by RV Lloyd (1990)
Macville MVE, Wiesmeijer KC, Dirks RW, Fransen JAM, Raap AK (1995) Saponin pre-treatment in pre-embedding electron microscopic in situ hybridization for the detection of specific RNA sequences in cultured cells: a methodological study. J Histochem Cytochem (in press) (10.1177/43.10.7560878)
Puvion-Dutilleul F, Bachellerie JP, Puvion E (1991) Nucleolar organization of HeLa cells as studied by in situ hybridization. Chromosoma 100:395–409 (10.1007/BF00337518) / Chromosoma by F Puvion-Dutilleul (1991)
Sibon OCM, Humbel BM, De Graaf A, Verkleij AJ, Cremers FFM (1994) Ultrastructural localization of epidermal growth factor (EGF)-receptor in the cell nucleus using pre-embedding in situ hybridization in combination with ultra-small gold probes and silver-enhancement. Histochemistry 101:223–232 (10.1007/BF00269548) / Histochemistry by OCM Sibon (1994)
Sibon OCM, Cremers FFM, Humbel BM, Boonstra J, Verkleij AJ (1995) Localization of nuclear RNA by pre- and post-embedding in situ hybridization using different gold probes. Histochem J 27:35–45 (10.1007/BF00164170) / Histochem J by OCM Sibon (1995)
Slot JW, Geuze HJ (1985) A novel method of preparing gold probes for multiple-labelling cytochemistry. Eur J Cell Biol 38:87–93 / Eur J Cell Biol by JW Slot (1985)
Stierhof YD, Humbel BM, Schwarz H (1991) Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: an immunoelectron microscopic study. J Electron Microsc Techn 17:336–343 (10.1002/jemt.1060170307) / J Electron Microsc Techn by YD Stierhof (1991)
Takizawa T, Robinson JM (1994) Use of 1,4-nm immunogold particles for immunocytochemistry on ultra-thin cryosections. J Histochem Cytochem 42:1615–1623 (10.1177/42.12.7983362) / J Histochem Cytochem by T Takizawa (1994)
Thiry M, Thiry-Blaise L (1989) In situ hybridization at the electron microscope level: an improved method for precise localization of ribosomal DNA and RNA. Eur J Cell Biol 50:235–243 / Eur J Cell Biol by M Thiry (1989)
Tokuyasu KT (1984) Immuno-cryoultramicrotomy. In: Polak JM, Varndell IM (eds) Immunolabelling for electron microscopy, Elsevier, Amsterdam, pp 71–82 / Immunolabelling for electron microscopy by KT Tokuyasu (1984)
Van Bergen en Henegouwen PMP (1989) Immunogold labeling of ultrathin cryosections. In: Hayat MA (ed) Colloidal gold: principles, methods, and applications, vol 1. Academic Press, New York, pp 191–216 (10.1016/B978-0-12-333927-0.50011-8) / Colloidal gold: principles, methods, and applications, vol 1 by Bergen Van (1989)
Wachtler F, Schöfer C, Mosgöller W, Weipoltshammer K, Schwarzacher HG, Guichaoua M, Hartung M, Stahl A, Bergé-Lefranc JL, Gonzalez I, Sylvester J (1992) Human ribosomal RNA gene repeats are localized in the dense fibrillar component of the nucleoli—light and electron microscopic in situ hybridization in human, Sertoli cells. Exp Cell Res 198:135–143 (10.1016/0014-4827(92)90159-6) / Sertoli cells. Exp Cell Res by F Wachtler (1992)
Webster HF, Lamperth L, Favilla JT, Lemke G, Tesin D, Manuclidis L (1987) Use of biotinylated probe and in situ hybridization for light and electron microscopic localization of P0 mRNA in myelin-forming Schwann cells. Histochemistry 86:441–444 (10.1007/BF00500614) / Histochemistry by HF Webster (1987)
Wenderoth MP, Eisenberg BR (1991) Ultrastructural distribution of myosin heavy chain mRNA in cardiac tissue: a comparison of frozen and LR White embedment. J Histochem Cytochem 39:1025–1033 (10.1177/39.8.1856452) / J Histochem Cytochem by MP Wenderoth (1991)
Wolber RA, Beals TF, Maassab HF (1989) Ultrastructural localization of Herpes simplex virus RNA by in situ hybridization. J Histochem Cytochem 37:97–104 (10.1177/37.1.2535704) / J Histochem Cytochem by RA Wolber (1989)
Yoshii A, Koji T, Ohsawa N, Nakane PK (1995) In situ localization of ribosomal RNAs is a reliable reference for hybridizable RNA in tissue sections. J Histochem Cytochem 43:321–327 (10.1177/43.3.7532657) / J Histochem Cytochem by A Yoshii (1995)

Dates

Type	When
Created	20 years, 3 months ago (May 15, 2005, 3:06 a.m.)
Deposited	5 years, 4 months ago (April 7, 2020, 6:17 a.m.)
Indexed	3 years, 5 months ago (March 31, 2022, 8:02 a.m.)
Issued	29 years, 7 months ago (Feb. 1, 1996)
Published	29 years, 7 months ago (Feb. 1, 1996)
Published Print	29 years, 7 months ago (Feb. 1, 1996)

Funders 0

None

BibTeX

@article{Macville_1996, title={Evaluation of pepsin treatment for electron microscopic RNA in situ hybridization on ultra-thin cryosections of cultured cells}, volume={105}, ISSN={1432-119X}, url={http://dx.doi.org/10.1007/bf01696153}, DOI={10.1007/bf01696153}, number={2}, journal={Histochemistry and Cell Biology}, publisher={Springer Science and Business Media LLC}, author={Macville, Merryn V. E. and Dorp, Annette G. M. and Dirks, Roeland W. and Fransen, Jack A. M. and Raap, Anton K.}, year={1996}, month=feb, pages={139–145} }

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