DOI: 10.1007/bf01122461. Trypsin digestion of core chromatin

10.1007/bf01122461

Crossref journal-article

Portland Press Ltd.

Bioscience Reports (288)

Abstract

Chicken erythrocyte core chromatin was digested with trypsin for 18 h. Five major limit petides were produced with mol. wts, from 10 800 to 8000 which arose from the central regions of each core histone. The basic amino- and carboxyl-terminal regions of each core histone were digested to small peptides having an average size of less than six amino acids. The small basic peptides, 25% by weight of the total histone, dissociated from the complex and could be removed by dialysis. The five major limit peptides remained bound to the DNA and contained all the secondary structure originally present in the native histones. Trypsin digestion decreased the supercoiling of the DNA in the complex and perturbed the tertiary structure of the histones. By contrast, there were no changes in the secondary structure of the large degraded histone fragments. However, when these were dissociated from the DNA, the secondary structure, which is predominantly α-helix, decreased by 50%. It is concluded that DNA binds strongly to the central regions of the core histones via α-helical segments on the polypeptide chains.

Bibliography

Diaz, B. M., & Walker, I. O. (1983). Trypsin digestion of core chromatin. Bioscience Reports, 3(3), 283â292.

Authors 2

B. M. Diaz (first)
I. O. Walker (additional)

References 32 Referenced 8

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Dates

Type	When
Created	20 years, 6 months ago (Feb. 6, 2005, 4:29 a.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 12:57 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 3:08 p.m.)
Issued	42 years, 5 months ago (March 1, 1983)
Published	42 years, 5 months ago (March 1, 1983)
Published Print	42 years, 5 months ago (March 1, 1983)

Funders 0

None

BibTeX

@article{Diaz_1983, title={Trypsin digestion of core chromatin}, volume={3}, ISSN={1573-4935}, url={http://dx.doi.org/10.1007/bf01122461}, DOI={10.1007/bf01122461}, number={3}, journal={Bioscience Reports}, publisher={Portland Press Ltd.}, author={Diaz, B. M. and Walker, I. O.}, year={1983}, month=mar, pages={283–292} }

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