Immunochemistry on ultrathin frozen sections
10.1007/bf01011956
Crossref journal-article
Springer Science and Business Media LLC
The Histochemical Journal (297)
Bibliography

Tokuyasu, K. T. (1980). Immunochemistry on ultrathin frozen sections. The Histochemical Journal, 12(4), 381–403.

Authors 1
  1. K. T. Tokuyasu (first)
References 60 Referenced 552
  1. Appleton, T. C. (1969) The possibilities of locating soluble labeled compounds by electron microscope autoradiography. InAutoradiography of Diffusible Substances (edited byStumpf, W. E. andRoth, L. J.), p. 301. New York: Academic Press. / Autoradiography of Diffusible Substances by T. C. Appleton (1969)
  2. Apeleton, T. C. (1974) A cryostat approach to ultrathin ?dry? frozen sections for electron microscopy: a morphological and X-ray analytical study.J. Microscopy 100, 49?74. (10.1111/j.1365-2818.1974.tb03913.x) / J. Microscopy by T. C. Apeleton (1974)
  3. Avrameas, S. (1969) Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.Immunochemistry 6, 43?52. (10.1016/0019-2791(69)90177-3) / Immunochemistry by S. Avrameas (1969)
  4. Avrameas, S. &Uriel, J. (1966) Méthode de marquage d'antigènes et d'anticorps avec des enzymes et son application en immunodiffusion.C.r. hebd. Séanc. Acad. Sci. Paris, Ser.D,262, 2543?5. / C.r. hebd. Séanc. Acad. Sci. Paris, Ser.D by S. Avrameas (1966)
  5. Bartlett, P. A., Bauer, B. &Singer, S. J. (1978) Synthesis of water-soluble undecagold cluster compounds of potential importance in electron microscopic and other studies of biological systems.J. Am. chem. Soc. 100, 5085?9. (10.1021/ja00484a029) / J. Am. chem. Soc. by P. A. Bartlett (1978)
  6. Baumgartner, B., Tokuyasu, K. T. &Chrispeels, M. J. (1978) Localization of vicilin peptidohydrolase in the cotyledons of mung bean seedlings by immunofluorescence microscopy.J. Cell Biol. 79, 10?9. (10.1083/jcb.79.1.10) / J. Cell Biol. by B. Baumgartner (1978)
  7. Bernhard, W. &Leduc, E. H. (1967) Ultrathin frozen sections. I. Methods and ultrastructural preservation.J. Cell Biol. 34, 757?71. (10.1083/jcb.34.3.757) / J. Cell Biol. by W. Bernhard (1967)
  8. Bernhard, W. &Nancy, M. T. (1964) Coupes a congelation ultrafines de tissus inclus dans la gelatine.J. Microscopy 3, 579. / J. Microscopy by W. Bernhard (1964)
  9. Bernhard, W. &Viron, A. (1971) Improved techniques for the preparation of ultrathin frozen sections.J. Cell Biol. 49, 731?46. (10.1083/jcb.49.3.731) / J. Cell Biol. by W. Bernhard (1971)
  10. Bernier, R., Iglesias, R. &Simard, R. (1972) Detection of DNA by tritiated actinomycin D on ultrathin frozen sections.J. Cell Biol. 53, 798?808. (10.1083/jcb.53.3.798) / J. Cell Biol. by R. Bernier (1972)
  11. Beyer, E. C., Tokuyasu, K. T. &Barondes, S. H. (1979) Localization of an endogenous lectin in chicken liver, intestine and pancreas.J. Cell Biol. 82, 565?71. (10.1083/jcb.82.2.565) / J. Cell Biol. by E. C. Beyer (1979)
  12. Bourguignon, L. Y. W., Tokuyasu, K. T. &Singer, S. J. (1978) The capping of lymphocytes and other cells, studied by an improved method of immunofluorescence staining of frozen sections.J. Cell Physiol. 95, 239?57. (10.1002/jcp.1040950302) / J. Cell Physiol. by L. Y. W. Bourguignon (1978)
  13. Christensen, A. K. (1969) A way to prepare frozen thin sections of fresh tissue for electron microscopy. InAutoradiography of Diffusible Substances (edited byStumpf, W. E. andRoth, L. J.), p. 349. New York: Academic Press. / Autoradiography of Diffusible Substances by A. K. Christensen (1969)
  14. Christensen, A. K. (1971) Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver.J. Cell Biol. 51, 772?804. (10.1083/jcb.51.3.772) / J. Cell Biol. by A. K. Christensen (1971)
  15. De Petris, S. (1978) Immunoelectron microscopy and immunofluorescence in membrane biology. InMethods in Membrane Biology (edited byKorn, E. D.), Vol. 9, pp. 1?201. New York: Plenum Press. / Methods in Membrane Biology by S. Petris De (1978)
  16. Dutton, A., Tokuyasu, K. T. &Singer, S. J. (1979) Iron-dextran antibody conjugates; a general method for the simultaneous staining of two components in high resolution immunoelectron microscopy.Proc. natn. Acad. Sci. U.S.A. 76, 3392?6. (10.1073/pnas.76.7.3392) / Proc. natn. Acad. Sci. U.S.A. by A. Dutton (1979)
  17. Fernández-Morán, H. (1952) The submicroscopic organization of vertebrate nerve fibres. An electron microscopic study of myelinated and unmyelinated nerve fibres.Expl. Cell Res. 3, 282?359. (10.1016/0014-4827(52)90128-6) / Expl. Cell Res. by H. Fernández-Morán (1952)
  18. Geiger, B., Dutton, A., Tokuyasu, K. T., & Singer, S. J. (1980a) Electron microscopic studies of cytoskeletal interaction. II. The distribution of tropomyosin, ?-actinin and vinculin in intestinal epithelial brush border. (in press).
  19. Geiger, B., Dutton, A., Tokuyasu, K. T. & Singer, S. J. (1980b) Electron microscopic studies of cytoskeletal interaction. III. The distribution of tropomyosin, ?-actinin, and vinculin in chicken gizzard smooth muscle cells. (in press).
  20. Geiger, B., Tokuyasu, K. T. &Singer, S. J. (1979) The immunocytochemical localization of ?-actinin in intestinal epithelial cells.Proc. natn. Acad. Sci. U.S.A. 76, 2833?7. (10.1073/pnas.76.6.2833) / Proc. natn. Acad. Sci. U.S.A. by B. Geiger (1979)
  21. Geuze, J. J., Slot, J. W. &Tokuyasu, K. T. (1979) Immunocytochemical localization of amylase and chymotrypsinogen in the exocrine pancreatic cell with special attention to the Golgi complex.J. Cell Biol. 82, 697?707. (10.1083/jcb.82.3.697) / J. Cell Biol. by J. J. Geuze (1979)
  22. Hellström, S. &Sjöström, M. (1974). An anti-roll plate for flattening of ?dry? ultrathin frozen sections.J. Microscopy 101, 197?200. (10.1111/j.1365-2818.1974.tb03882.x) / J. Microscopy by S. Hellström (1974)
  23. Horisberger, M. &Rosset, J. (1977) Colloidal gold, a useful marker for transmission and scanning electron microscopy.J. Histochem. Cytochem. 25, 295?305. (10.1177/25.4.323352) / J. Histochem. Cytochem. by M. Horisberger (1977)
  24. Iglesias, R., Bernier, R. &Simard, R. (1971) Ultracryotomy: A routine procedure.J. Ultrastruct. Res. 36, 271?89. (10.1016/S0022-5320(71)80104-1) / J. Ultrastruct. Res. by R. Iglesias (1971)
  25. Kawarai, Y. &Nakane, P. K. (1970) Localization of tissue antigens of the ultrathin sections with peroxidase-labeled antibody method.J. Histochem. Cytochem. 18, 161?6. (10.1177/18.3.161) / J. Histochem. Cytochem. by Y. Kawarai (1970)
  26. Kishida, Y., Olsen, B. R., Berg, R. A. &Prockop, D. J. (1975) Two improved methods for preparing ferritin-protein conjugates for electron microscopy.J. Cell Biol. 64, 331?9. (10.1083/jcb.64.2.331) / J. Cell Biol. by Y. Kishida (1975)
  27. Koller, T. (1965) Mounting of ultrathin sections with the aid of an electrostatic field.J. Cell Biol. 27, 441?5. (10.1083/jcb.27.2.441) / J. Cell Biol. by T. Koller (1965)
  28. Kraehenbuhl, J. P. &Jamieson, J. D. (1972) Solid phase conjugation of ferritin to Fab fragments for use in antigen localization on thin sections.Proc. natn. Acad. Sci. U.S.A. 69, 1771?5. (10.1073/pnas.69.7.1771) / Proc. natn. Acad. Sci. U.S.A. by J. P. Kraehenbuhl (1972)
  29. Kraehenbuhl, J. P., Racine, L. &Jamieson, J. D. (1977) Immunocytochemical localization of secretory proteins in bovine pancreatic exocrine cells.J. Cell. Biol. 72, 406?23. (10.1083/jcb.72.2.406) / J. Cell. Biol. by J. P. Kraehenbuhl (1977)
  30. Kyte, J. (1976a) Immunoferritin determination of the distribution of (Na+?K+) ATPase over the plasma membranes of renal convoluted tubules. I. Distal segment.J. Cell Biol. 68, 287?303. (10.1083/jcb.68.2.287) / J. Cell Biol. by J. Kyte (1976)
  31. Kyte, J. (1976b) Immunoferritin determination of the distribution of (Na+?K+) ATPase over the plasma membranes of renal convoluted tubules. II. Proximal segment.J. Cell Biol. 68, 304?18. (10.1083/jcb.68.2.304) / J. Cell Biol. by J. Kyte (1976)
  32. Leduc, E. H., Bernhard, W., Holt, S. J. &Tranzer, J. P. (1967) Ultrathin frozen sections. II. Demonstration of enzymatic activity.J. Cell Biol. 34, 773?86. (10.1083/jcb.34.3.773) / J. Cell Biol. by E. H. Leduc (1967)
  33. Lenard, J. &Singer, S. J. (1968) Alteration of the conformation of proteins in red cell membranes and in solution by fixatives used in electron microscopy.J. Cell Biol. 37, 117?21. (10.1083/jcb.37.1.117) / J. Cell Biol. by J. Lenard (1968)
  34. Mclean, J. D. &Singer, S. J. (1970) A general method for the specific staining of intracellular antigens with ferritin-antibody conjugates.Proc. natn. Acad. Sci. U.S.A. 65, 122?8. (10.1073/pnas.65.1.122) / Proc. natn. Acad. Sci. U.S.A. by J. D. Mclean (1970)
  35. Nakane, P. K. (1970) Classification of anterior pituitary cell types with immunoenzyme histochemistry.J. Histochem. Cytochem. 18, 9?20. (10.1177/18.1.9) / J. Histochem. Cytochem. by P. K. Nakane (1970)
  36. Nakane, P. K. &Pierce, G. B., Jr., (1966) Enzyme-labeled antibodies: preparation and application for the localization of antigens.J. Histochem. Cytochem. 14, 929?31. (10.1177/14.12.929) / J. Histochem. Cytochem. by P. K. Nakane (1966)
  37. Nakane, P. K. &Pierce, G. B. Jr., (1967) Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigen.J. Cell Biol. 33, 307?18. (10.1083/jcb.33.2.307) / J. Cell Biol. by P. K. Nakane (1967)
  38. Olsen, B. R., Berg, R. A., Kishida, Y. &Prockop, D. J. (1975) Further characterization of embryonic tendon fibroblasts and the use of immunoferritin techniques to study collagen biosynthesis.J. Cell Biol. 64, 340?55. (10.1083/jcb.64.2.340) / J. Cell Biol. by B. R. Olsen (1975)
  39. Osborn, M., Webster, R. E. &Weber, K. (1978) Individual microtubules viewed by immunofluorescence and electron microscopy in the same PtK2 cell.J. Cell Biol. 77, R27?33. (10.1083/jcb.77.3.R27) / J. Cell Biol. by M. Osborn (1978)
  40. Fainter, R. G., Tokuyasu, K. T. &Singer, S. J. (1973) Immunoferritin localization of intracellular antigens: the use of ultracryotomy to obtain ultrathin sections suitable for direct immunoferritin staining.Proc. natn. Acad. Sci. U.S.A. 70, 1649?53. (10.1073/pnas.70.6.1649) / Proc. natn. Acad. Sci. U.S.A. by R. G. Fainter (1973)
  41. Papermaster, D. S., Schneider, B. G., Zorn, M. A. &Kraehenbuhl, J. P. (1978a) Immunocytochemical localization of opsin in outer segments and Golgi zones of frog photoreceptor cells.J. Cell Biol. 77, 196?210. (10.1083/jcb.77.1.196) / J. Cell Biol. by D. S. Papermaster (1978)
  42. Papermaster, D. S., Schneider, B. G., Zorn, M. A. &Kraehenbuhl, J. P. (1978b) Immunocytochemical localization of a large intrinsic membrane protein to the incisures and margins of frog rod outer segment disks.J. Cell Biol. 78, 415?25. (10.1083/jcb.78.2.415) / J. Cell Biol. by D. S. Papermaster (1978)
  43. Rebdy, M. K. &Barkas, A. E. (1974) Disordeting of myofibril structure due to fixation, dehydration and embedding.J. Cell Biol. 63, 282a. / J. Cell Biol. by M. K. Rebdy (1974)
  44. Sabatini, D. D., Bensh, K. &Barrnett, R. J. (1963) Cytochemistry and electron microscopy: The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.J. Cell Biol. 17, 19?58. (10.1083/jcb.17.1.19) / J. Cell Biol. by D. D. Sabatini (1963)
  45. Singer, S. J. (1959) Preparation of an electron-dense antibody conjugate.Nature, Lond. 183, 1523?4. (10.1038/1831523a0) / Nature, Lond. by S. J. Singer (1959)
  46. Singer, S. J., Painter, R. G. &Tokuyasu, K. T. (1974) Immunoelectron microscopy with ferritin conjugates.Eighth Int. Congr. Electr. Microsc. 2, 108?9. / Eighth Int. Congr. Electr. Microsc. by S. J. Singer (1974)
  47. Singer, S. J. &Schick, A. F. (1961) The properties of specific stains for electron microscopy prepared by the conjugation of antibody molecules with ferritin.J. biophys. biochem. Cytol. 9, 519?37. (10.1083/jcb.9.3.519) / J. biophys. biochem. Cytol. by S. J. Singer (1961)
  48. Sjöstrand, F. S. &Bernhard, W. (1976) The structure of mitochondrial membranes in frozen sections.J. Ultrastruct. Res. 56, 233?46. (10.1016/S0022-5320(76)80169-4) / J. Ultrastruct. Res. by F. S. Sjöstrand (1976)
  49. Sjöström, M. &Squire, J. M. (1977) Fine structure of the A-band in cryosections. The structure of the A-band of human skeletal muscle fibres from ultra-thin cryo-section negatively stained.J. molec. Biol. 109, 49?68. (10.1016/S0022-2836(77)80045-4) / J. molec. Biol. by M. Sjöström (1977)
  50. Stasny, J. T., Burns, R. C. & Hardy, R. W. F. (1973) The immuno-electron microscopic localization on the Mo?Fe protein component of nitrogenase in cells ofAzotobacter vinelandii. 31st Ann. Meet. Electr. Microsc. Soc. Am., pp. 574?5. (10.1017/S0424820100073313)
  51. Sternberger, L. A. (1973) Enzyme immunocytochemistry. InElectron Microscopy of Enzymes (edited byHayat, M. A.), Vol. 1, p. 150. New York: Van Nostrand Reinhold. / Electron Microscopy of Enzymes by L. A. Sternberger (1973)
  52. Tokuyasu, K. T. (1973) A technique for ultracryotomy of cell suspensions and tissues.J. Cell Biol. 57, 551?65. (10.1083/jcb.57.2.551) / J. Cell Biol. by K. T. Tokuyasu (1973)
  53. Tokuyasu, K. T. (1974) Some applications of cryo-ultramicrotomy.Eighth Int. Congr. Electr. Microsc. 2, 34?5. / Eighth Int. Congr. Electr. Microsc. by K. T. Tokuyasu (1974)
  54. Tokuyasu, K. T. (1976) Membranes as observed in frozen sections.J. Ultrastruct. Res. 55, 281?7. (10.1016/S0022-5320(76)80073-1) / J. Ultrastruct. Res. by K. T. Tokuyasu (1976)
  55. Tokuyasu, K. T. (1978a) A study of positive staining of ultrathin frozen sections.J. Ultrastruct. Res. 63, 287?307. (10.1016/S0022-5320(78)80053-7) / J. Ultrastruct. Res. by K. T. Tokuyasu (1978)
  56. Tokuyasu, K. T. (1978b) Immunoferritin localization of intracellular antigens in positively stained frozen sections.Ninth Int. Congr. Electr. Microsc. 2, 168?9. / Ninth Int. Congr. Electr. Microsc. by K. T. Tokuyasu (1978)
  57. Tokuyasu, K. T., Schekman, R. &Singer, S. J. (1979) Domains of receptor mobility and endocytosis in the membranes of neonatal human erythrocytes and reticulocytes are deficient in spectrin.J. Cell Biol. 80, 481?5. (10.1083/jcb.80.2.481) / J. Cell Biol. by K. T. Tokuyasu (1979)
  58. Tokuyasu, K. T. &Singer, S. J. (1976) Improved procedures for immunoferritin labeling of ultrathin frozen sections.J. Cell Biol. 71, 894?906. (10.1083/jcb.71.3.894) / J. Cell Biol. by K. T. Tokuyasu (1976)
  59. Tokuyasu, K. T., Slot, J. W. &Singer, S. J. (1978) Simultaneous observations of immunolabeled frozen sections in LM and EM.Ninth Int. Congr. Electr. Microsc. 2, 164?5. / Ninth Int. Congr. Electr. Microsc. by K. T. Tokuyasu (1978)
  60. Whilingham, M. C., Yamada, S. S. &Pastan, I. (1978) Ultrastructural antibody localization of ?2 macroglobulin in membrane-limited vesicles in cultured cells.Proc. natn. Acad. Sci. U.S.A. 75, 4359?63. (10.1073/pnas.75.9.4359) / Proc. natn. Acad. Sci. U.S.A. by M. C. Whilingham (1978)
Dates
Type When
Created 20 years, 7 months ago (Jan. 19, 2005, 3:55 a.m.)
Deposited 4 years, 1 month ago (July 4, 2021, 12:26 a.m.)
Indexed 1 week, 1 day ago (Aug. 12, 2025, 5:56 p.m.)
Issued 45 years, 1 month ago (July 1, 1980)
Published 45 years, 1 month ago (July 1, 1980)
Published Print 45 years, 1 month ago (July 1, 1980)
Funders 0

None

@article{Tokuyasu_1980, title={Immunochemistry on ultrathin frozen sections}, volume={12}, ISSN={1573-6865}, url={http://dx.doi.org/10.1007/bf01011956}, DOI={10.1007/bf01011956}, number={4}, journal={The Histochemical Journal}, publisher={Springer Science and Business Media LLC}, author={Tokuyasu, K. T.}, year={1980}, month=jul, pages={381–403} }