DOI: 10.1007/bf01003288. The effect of different fixatives on chromatin: Cytochemical and ultrastructural approaches

The effect of different fixatives on chromatin: Cytochemical and ultrastructural approaches

10.1007/bf01003288

Crossref journal-article

Springer Science and Business Media LLC

The Histochemical Journal (297)

Bibliography

Fraschini, A., Pellicciari, C., Biggiogera, M., & Manfredi Romanini, M. G. (1981). The effect of different fixatives on chromatin: Cytochemical and ultrastructural approaches. The Histochemical Journal, 13(5), 763â779.

Authors 4

A. Fraschini (first)
C. Pellicciari (additional)
M. Biggiogera (additional)
M. G. Manfredi Romanini (additional)

References 43 Referenced 21

Bedi, K. S. &Goldstein, D. J. (1974) Cytophotometric factors causing apparent differences between Feulgen-DNA content of different leukocytes types.Nature, Lond. 251, 439?40. (10.1038/251439a0) / Nature, Lond. by K. S. Bedi (1974)
Bernhard, W. (1969) A new staining procedure for electron microscopical cytology.J. Ultrastruct. Res. 27, 250?65. (10.1016/S0022-5320(69)80016-X) / J. Ultrastruct. Res. by W. Bernhard (1969)
Böyum, A. (1964) Separation of white blood cells.Nature, Lond. 204, 793?94. (10.1038/204793a0) / Nature, Lond. by A. Böyum (1964)
Bowes, J. H. &Cater, C. W. (1966) The reaction of glutaraldehyde with proteins and other biological materials.J. R. microsc. Soc. 85, 193?200. (10.1111/j.1365-2818.1966.tb02180.x) / J. R. microsc. Soc. by J. H. Bowes (1966)
Brody, T. (1974) Histones in cytological preparations.Expl Cell. Res. 101, 255?63. (10.1016/0014-4827(74)90125-6) / Expl Cell. Res. by T. Brody (1974)
Diaz, M. (1972) Methyl Green staining and highly repetitive DNA in polytene chromosomes.Chromosoma 37, 131?8. (10.1007/BF00284935) / Chromosoma by M. Diaz (1972)
Dick, C. &Johns, W. (1968) The effect of two acetic acid containing fixatives on the histone content of calf thymus deoxyribonuclearprotein and calf thymus tissue.Expl Cell Res. 51, 626?32. (10.1016/0014-4827(68)90150-X) / Expl Cell Res. by C. Dick (1968)
Fraschini, A. &Marinozzi, V. (1977) Critical analysis of the use of the acrolein-Schiff method as a possible DNA reaction.Histochemistry 50, 197?206. (10.1007/BF00491067) / Histochemistry by A. Fraschini (1977)
Fraschini, A., Bolchi, F. &Biggiogera, M. (1980) Ultrastructural cytotopochemistry of chromatin: approaches via destructive deproteinizing.J. submicrosc. Cytol. 12, 121?36. / J. submicrosc. Cytol. by A. Fraschini (1980)
Gaub, J. (1976) Feulgen-Naphthol Yellow S cytophotometry of liver cells.Histochemistry 49, 293?301. (10.1007/BF00496132) / Histochemistry by J. Gaub (1976)
Haselkorn, R. &Doty, P. (1961) The reaction of formaldehyde with polynucleotides.J. Biol. Chem. 236, 2738?45. (10.1016/S0021-9258(19)61728-X) / J. Biol. Chem. by R. Haselkorn (1961)
Hayat, M. A. (1970)Principles and Techniques of Electron Microscopy. Vol. 1. pp. 35?107. New York: Van Nostrand Reinhold. / Principles and Techniques of Electron Microscopy by M. A. Hayat (1970)
Hopwood, D. (1968) Some aspects of fixation by glutaraldehyde and formaldehyde.J. Anat., Lond. 103, 581?89. / J. Anat., Lond. by D. Hopwood (1968)
Itikawa, O. &Ogura, Y. (1954) The Feulgen reaction after hydrolysis at room temperature.Stain. Technol. 29, 237?49. / Stain. Technol. by O. Itikawa (1954)
Kiefer, R., Kiefer, G. &Sandritter, W. (1972) Feulgenhidrolyse kinetic in eu- und eterochromatin.Histochemie 30, 150?5. (10.1007/BF01444061) / Histochemie by R. Kiefer (1972)
Kjellstrand, P. T. T. &Anderssen, G. K. A. (1975) Histochemical properties of spermatozoa and somatic cells. I: Relations between Feulgen hydrolysis pattern and the composition of the nucleoproteins.Histochem. J. 7, 563?73. (10.1007/BF01003794) / Histochem. J. by P. T. T. Kjellstrand (1975)
Le Boutellier, P., Kinsky, R., Vujanovic, N., Duc, H. &Voisin, G. (1976) Morphological differences between thymus and bone marrow derived lymphocytes.Differentiation 6, 125?41. (10.1111/j.1432-0436.1976.tb01479.x) / Differentiation by P. Boutellier Le (1976)
Le Boutellier, P., Kinsky, R., Righenzi, S. &Voisin, G. (1978) Electron microscopic study of thymus and bone marrow derived mouse lymphocytes.Ann. Immun. 129, 635?51. / Ann. Immun. by P. Boutellier Le (1978)
Luft, J. (1959) The use of acrolein as a fixative for light and electron microscopy.Anat. Rec. 133, 305?12. / Anat. Rec. by J. Luft (1959)
Manfrediromanini, M. G., Formenti, D., Fraschini, A., Pellicciari, C., Redi, C. A. &Scherini, E. (1981) Some parameters for a cell cycle cytotopochemical study.Gegenbaurs morphol. Jahrb., Leipzig 127, 581?7. / Gegenbaurs morphol. Jahrb., Leipzig by M. G. Manfrediromanini (1981)
Marinozzi, V. (1963) The role of fixation in electron staining.J. R. microsc. Soc. 81, 141?54. (10.1111/j.1365-2818.1963.tb02083.x) / J. R. microsc. Soc. by V. Marinozzi (1963)
Mayall, B. (1966) The detection of small differences in DNA content with microspectrophotometric techniques.J. Cell Biol. 31, 74A. / J. Cell Biol. by B. Mayall (1966)
Mayall, B. &Mendelsohn, M. (1967) Chromatin and chromosome compaction and the stoichiometry of DNA staining.J. Cell Biol. 35, 88A. / J. Cell Biol. by B. Mayall (1967)
Mayall, B. (1969) Deoxyribonucleic acid cytophotometry of stained human leukocytes. I. Differences among cell types.J. Histochem. Cytochem. 17, 249?57. (10.1177/17.4.249) / J. Histochem. Cytochem. by B. Mayall (1969)
Millonig, G. &Marinozzi, V. (1968) Fixation and embedding in electron microscopy. InAdvances in Optical and Electron Microscopy (edited byBarer, R. &Cosslett, V.) Vol. 2. pp. 251?341. London: Academic Press. / Advances in Optical and Electron Microscopy by G. Millonig (1968)
Mittermayer, C., Madreiter, H., Lederer, B. &Sandritter, W. (1971) Differential acid hydrolysis of euchromatin and heterochromatin.Beitr. Path. 143, 157?65. / Beitr. Path. by C. Mittermayer (1971)
Nicolini, C. (1979)Chromatin structure and function. Vol. A. pp. 293?322. New York: Plenum Press. (10.1007/978-1-4684-0973-4_14) / Chromatin structure and function by C. Nicolini (1979)
Olins, D. &Wright, E. (1973) Glutaraldehyde fixation of isolated eucaryotic nuclei.J. Cell Biol. 59, 304?17. (10.1083/jcb.59.2.304) / J. Cell Biol. by D. Olins (1973)
Pellicciari, C., Manfredi Romanini, M. G., Casirola, G., Ippoliti, G. & Marini, G. (1976) Hydrolysis kinetics studies of the Feulgen reaction on different lymphocyte populations in man.Fifth Int. Congr. Histochem. Cytochem., Bucharest.
Pellicciari, C. &Fraschini, A. (1978) Methods of denaturation and renaturation of DNA in interphasic chromatin: cytochemical quantitative analysis by Methyl Green staining.Histochem. J. 10, 213?22. (10.1007/BF01003306) / Histochem. J. by C. Pellicciari (1978)
Rasch, R. &Rasch, E. (1973) Kinetics of hydrolysis during the Feulgen reaction for deoxyribonucleic acid. A re-evaluation.J. Histochem. Cytochem. 21, 1053?65. (10.1177/21.12.1053) / J. Histochem. Cytochem. by R. Rasch (1973)
Retief, A. &Rüchel, R. (1977) Histones removed by fixation. Their role in the mechanism of chromosomal banding.Expl. Cell Res. 106, 233?7. (10.1016/0014-4827(77)90260-9) / Expl. Cell Res. by A. Retief (1977)
Reynolds, E. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.J. Cell Biol. 17, 208?13. (10.1083/jcb.17.1.208) / J. Cell Biol. by E. Reynolds (1963)
Sabatini, D., Bensch, K. &Barrnett, R. (1962) New means of fixation for electron microscopy and histochemistry.Anat. Rec. 142, 274?90. / Anat. Rec. by D. Sabatini (1962)
Sabatini, D., Bensch, K. &Barrnett, R. (1963) Cytochemistry and electron microscopy: the preservation of the cellular ultrastructure and enzymatic activity by aldehyde fixation.J. Cell Biol. 17, 19?58. (10.1083/jcb.17.1.19) / J. Cell Biol. by D. Sabatini (1963)
Sabatini, D., Miller, F. &Barrnett, R. (1964) Aldehyde fixation for morphological and enzyme histochemical studies with the electron microscope.J. Histochem. Cytochem. 12, 57?71. (10.1177/12.2.57) / J. Histochem. Cytochem. by D. Sabatini (1964)
Scott, J. (1967) On the mechanism of the Methyl Green-pyronine stain for nucleic acids.Histochemie 9, 30?46. (10.1007/BF00281805) / Histochemie by J. Scott (1967)
Stollar, D. &Grossman, L. (1962) The reaction of formaldehyde with denatured DNA: spectrophotometric, immunologic and enzymic studies.J. molec. Biol. 4, 31?8. (10.1016/S0022-2836(62)80114-4) / J. molec. Biol. by D. Stollar (1962)
Sumner, A., Evans, H. &Buckland, R. (1973) Mechanism involved in the banding of chromosomes with Quinacrine and Giemsa. I. The effects of fixation in methanol-acetic acid.Expl. Cell Res. 81, 214?22. (10.1016/0014-4827(73)90128-6) / Expl. Cell Res. by A. Sumner (1973)
Trump, B. F. &Ericsson, J. L. (1965) The effect of the fixative solution on the ultrastructure of cells and tissues. A comparative analysis with particular attention to the proximal convoluted tubule of the rat kidney.Lab. Invest. 14, 1245?51. / Lab. Invest. by B. F. Trump (1965)
Trump, B. F. &Bulger, R. E. (1966) New ultrastructural characteristics of cells fixed in a glutaraldehyde-osmium tetroxide mixture.Lab. Invest. 15, 368?79. / Lab. Invest. by B. F. Trump (1966)
Van Duijn, P. (1961) Acrolein-Schiff: a new staining method for proteins.J. Histochem. Cytochem. 9, 234?41. (10.1177/9.3.234) / J. Histochem. Cytochem. by P. Duijn Van (1961)
Zetterberg, A. &Auer, G. (1969) Early changes in the binding between DNA and histones in human leukocytes exposed to phytoemagglutinin.Exp. Cell Res. 56, 122?6. (10.1016/0014-4827(69)90404-2) / Exp. Cell Res. by A. Zetterberg (1969)

Dates

Type	When
Created	20 years, 7 months ago (Jan. 19, 2005, 1:19 a.m.)
Deposited	4 years, 1 month ago (July 4, 2021, 12:04 a.m.)
Indexed	1 year, 10 months ago (Oct. 21, 2023, 8:56 p.m.)
Issued	43 years, 11 months ago (Sept. 1, 1981)
Published	43 years, 11 months ago (Sept. 1, 1981)
Published Print	43 years, 11 months ago (Sept. 1, 1981)

Funders 0

None

BibTeX

@article{Fraschini_1981, title={The effect of different fixatives on chromatin: Cytochemical and ultrastructural approaches}, volume={13}, ISSN={1573-6865}, url={http://dx.doi.org/10.1007/bf01003288}, DOI={10.1007/bf01003288}, number={5}, journal={The Histochemical Journal}, publisher={Springer Science and Business Media LLC}, author={Fraschini, A. and Pellicciari, C. and Biggiogera, M. and Manfredi Romanini, M. G.}, year={1981}, month=sep, pages={763–779} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        22
      ]
    ],
    "date-time": "2023-10-22T00:56:45Z",
    "timestamp": 1697936205363
  },
  "reference-count": 43,
  "publisher": "Springer Science and Business Media LLC",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1981,
            9,
            1
          ]
        ],
        "date-time": "1981-09-01T00:00:00Z",
        "timestamp": 368150400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "http://www.springer.com/tdm"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1981,
        9
      ]
    ]
  },
  "DOI": "10.1007/bf01003288",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        1,
        19
      ]
    ],
    "date-time": "2005-01-19T06:19:41Z",
    "timestamp": 1106115581000
  },
  "page": "763-779",
  "source": "Crossref",
  "is-referenced-by-count": 21,
  "title": "The effect of different fixatives on chromatin: Cytochemical and ultrastructural approaches",
  "prefix": "10.1007",
  "volume": "13",
  "author": [
    {
      "given": "A.",
      "family": "Fraschini",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "C.",
      "family": "Pellicciari",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M.",
      "family": "Biggiogera",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M. G.",
      "family": "Manfredi Romanini",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "297",
  "reference": [
    {
      "key": "CR1",
      "doi-asserted-by": "crossref",
      "first-page": "439",
      "DOI": "10.1038/251439a0",
      "volume": "251",
      "author": "K. S. Bedi",
      "year": "1974",
      "unstructured": "Bedi, K. S. &Goldstein, D. J. (1974) Cytophotometric factors causing apparent differences between Feulgen-DNA content of different leukocytes types.Nature, Lond. 251, 439?40.",
      "journal-title": "Nature, Lond."
    },
    {
      "key": "CR2",
      "doi-asserted-by": "crossref",
      "first-page": "250",
      "DOI": "10.1016/S0022-5320(69)80016-X",
      "volume": "27",
      "author": "W. Bernhard",
      "year": "1969",
      "unstructured": "Bernhard, W. (1969) A new staining procedure for electron microscopical cytology.J. Ultrastruct. Res. 27, 250?65.",
      "journal-title": "J. Ultrastruct. Res."
    },
    {
      "key": "CR3",
      "doi-asserted-by": "crossref",
      "first-page": "793",
      "DOI": "10.1038/204793a0",
      "volume": "204",
      "author": "A. B\u00f6yum",
      "year": "1964",
      "unstructured": "B\u00f6yum, A. (1964) Separation of white blood cells.Nature, Lond. 204, 793?94.",
      "journal-title": "Nature, Lond."
    },
    {
      "key": "CR4",
      "doi-asserted-by": "crossref",
      "first-page": "193",
      "DOI": "10.1111/j.1365-2818.1966.tb02180.x",
      "volume": "85",
      "author": "J. H. Bowes",
      "year": "1966",
      "unstructured": "Bowes, J. H. &Cater, C. W. (1966) The reaction of glutaraldehyde with proteins and other biological materials.J. R. microsc. Soc. 85, 193?200.",
      "journal-title": "J. R. microsc. Soc."
    },
    {
      "key": "CR5",
      "doi-asserted-by": "crossref",
      "first-page": "255",
      "DOI": "10.1016/0014-4827(74)90125-6",
      "volume": "101",
      "author": "T. Brody",
      "year": "1974",
      "unstructured": "Brody, T. (1974) Histones in cytological preparations.Expl Cell. Res. 101, 255?63.",
      "journal-title": "Expl Cell. Res."
    },
    {
      "key": "CR6",
      "doi-asserted-by": "crossref",
      "first-page": "131",
      "DOI": "10.1007/BF00284935",
      "volume": "37",
      "author": "M. Diaz",
      "year": "1972",
      "unstructured": "Diaz, M. (1972) Methyl Green staining and highly repetitive DNA in polytene chromosomes.Chromosoma 37, 131?8.",
      "journal-title": "Chromosoma"
    },
    {
      "key": "CR7",
      "doi-asserted-by": "crossref",
      "first-page": "626",
      "DOI": "10.1016/0014-4827(68)90150-X",
      "volume": "51",
      "author": "C. Dick",
      "year": "1968",
      "unstructured": "Dick, C. &Johns, W. (1968) The effect of two acetic acid containing fixatives on the histone content of calf thymus deoxyribonuclearprotein and calf thymus tissue.Expl Cell Res. 51, 626?32.",
      "journal-title": "Expl Cell Res."
    },
    {
      "key": "CR8",
      "doi-asserted-by": "crossref",
      "first-page": "197",
      "DOI": "10.1007/BF00491067",
      "volume": "50",
      "author": "A. Fraschini",
      "year": "1977",
      "unstructured": "Fraschini, A. &Marinozzi, V. (1977) Critical analysis of the use of the acrolein-Schiff method as a possible DNA reaction.Histochemistry 50, 197?206.",
      "journal-title": "Histochemistry"
    },
    {
      "key": "CR9",
      "first-page": "121",
      "volume": "12",
      "author": "A. Fraschini",
      "year": "1980",
      "unstructured": "Fraschini, A., Bolchi, F. &Biggiogera, M. (1980) Ultrastructural cytotopochemistry of chromatin: approaches via destructive deproteinizing.J. submicrosc. Cytol. 12, 121?36.",
      "journal-title": "J. submicrosc. Cytol."
    },
    {
      "key": "CR10",
      "doi-asserted-by": "crossref",
      "first-page": "293",
      "DOI": "10.1007/BF00496132",
      "volume": "49",
      "author": "J. Gaub",
      "year": "1976",
      "unstructured": "Gaub, J. (1976) Feulgen-Naphthol Yellow S cytophotometry of liver cells.Histochemistry 49, 293?301.",
      "journal-title": "Histochemistry"
    },
    {
      "key": "CR11",
      "doi-asserted-by": "crossref",
      "first-page": "2738",
      "DOI": "10.1016/S0021-9258(19)61728-X",
      "volume": "236",
      "author": "R. Haselkorn",
      "year": "1961",
      "unstructured": "Haselkorn, R. &Doty, P. (1961) The reaction of formaldehyde with polynucleotides.J. Biol. Chem. 236, 2738?45.",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "CR12",
      "first-page": "35",
      "volume-title": "Principles and Techniques of Electron Microscopy",
      "author": "M. A. Hayat",
      "year": "1970",
      "unstructured": "Hayat, M. A. (1970)Principles and Techniques of Electron Microscopy. Vol. 1. pp. 35?107. New York: Van Nostrand Reinhold."
    },
    {
      "key": "CR13",
      "first-page": "581",
      "volume": "103",
      "author": "D. Hopwood",
      "year": "1968",
      "unstructured": "Hopwood, D. (1968) Some aspects of fixation by glutaraldehyde and formaldehyde.J. Anat., Lond. 103, 581?89.",
      "journal-title": "J. Anat., Lond."
    },
    {
      "key": "CR14",
      "first-page": "237",
      "volume": "29",
      "author": "O. Itikawa",
      "year": "1954",
      "unstructured": "Itikawa, O. &Ogura, Y. (1954) The Feulgen reaction after hydrolysis at room temperature.Stain. Technol. 29, 237?49.",
      "journal-title": "Stain. Technol."
    },
    {
      "key": "CR15",
      "doi-asserted-by": "crossref",
      "first-page": "150",
      "DOI": "10.1007/BF01444061",
      "volume": "30",
      "author": "R. Kiefer",
      "year": "1972",
      "unstructured": "Kiefer, R., Kiefer, G. &Sandritter, W. (1972) Feulgenhidrolyse kinetic in eu- und eterochromatin.Histochemie 30, 150?5.",
      "journal-title": "Histochemie"
    },
    {
      "key": "CR16",
      "doi-asserted-by": "crossref",
      "first-page": "563",
      "DOI": "10.1007/BF01003794",
      "volume": "7",
      "author": "P. T. T. Kjellstrand",
      "year": "1975",
      "unstructured": "Kjellstrand, P. T. T. &Anderssen, G. K. A. (1975) Histochemical properties of spermatozoa and somatic cells. I: Relations between Feulgen hydrolysis pattern and the composition of the nucleoproteins.Histochem. J. 7, 563?73.",
      "journal-title": "Histochem. J."
    },
    {
      "key": "CR17",
      "doi-asserted-by": "crossref",
      "first-page": "125",
      "DOI": "10.1111/j.1432-0436.1976.tb01479.x",
      "volume": "6",
      "author": "P. Boutellier Le",
      "year": "1976",
      "unstructured": "Le Boutellier, P., Kinsky, R., Vujanovic, N., Duc, H. &Voisin, G. (1976) Morphological differences between thymus and bone marrow derived lymphocytes.Differentiation 6, 125?41.",
      "journal-title": "Differentiation"
    },
    {
      "key": "CR18",
      "first-page": "635",
      "volume": "129",
      "author": "P. Boutellier Le",
      "year": "1978",
      "unstructured": "Le Boutellier, P., Kinsky, R., Righenzi, S. &Voisin, G. (1978) Electron microscopic study of thymus and bone marrow derived mouse lymphocytes.Ann. Immun. 129, 635?51.",
      "journal-title": "Ann. Immun."
    },
    {
      "key": "CR19",
      "first-page": "305",
      "volume": "133",
      "author": "J. Luft",
      "year": "1959",
      "unstructured": "Luft, J. (1959) The use of acrolein as a fixative for light and electron microscopy.Anat. Rec. 133, 305?12.",
      "journal-title": "Anat. Rec."
    },
    {
      "key": "CR20",
      "first-page": "581",
      "volume": "127",
      "author": "M. G. Manfrediromanini",
      "year": "1981",
      "unstructured": "Manfrediromanini, M. G., Formenti, D., Fraschini, A., Pellicciari, C., Redi, C. A. &Scherini, E. (1981) Some parameters for a cell cycle cytotopochemical study.Gegenbaurs morphol. Jahrb., Leipzig 127, 581?7.",
      "journal-title": "Gegenbaurs morphol. Jahrb., Leipzig"
    },
    {
      "key": "CR21",
      "doi-asserted-by": "crossref",
      "first-page": "141",
      "DOI": "10.1111/j.1365-2818.1963.tb02083.x",
      "volume": "81",
      "author": "V. Marinozzi",
      "year": "1963",
      "unstructured": "Marinozzi, V. (1963) The role of fixation in electron staining.J. R. microsc. Soc. 81, 141?54.",
      "journal-title": "J. R. microsc. Soc."
    },
    {
      "key": "CR22",
      "first-page": "74A",
      "volume": "31",
      "author": "B. Mayall",
      "year": "1966",
      "unstructured": "Mayall, B. (1966) The detection of small differences in DNA content with microspectrophotometric techniques.J. Cell Biol. 31, 74A.",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "CR23",
      "first-page": "88A",
      "volume": "35",
      "author": "B. Mayall",
      "year": "1967",
      "unstructured": "Mayall, B. &Mendelsohn, M. (1967) Chromatin and chromosome compaction and the stoichiometry of DNA staining.J. Cell Biol. 35, 88A.",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "CR24",
      "doi-asserted-by": "crossref",
      "first-page": "249",
      "DOI": "10.1177/17.4.249",
      "volume": "17",
      "author": "B. Mayall",
      "year": "1969",
      "unstructured": "Mayall, B. (1969) Deoxyribonucleic acid cytophotometry of stained human leukocytes. I. Differences among cell types.J. Histochem. Cytochem. 17, 249?57.",
      "journal-title": "J. Histochem. Cytochem."
    },
    {
      "key": "CR25",
      "first-page": "251",
      "volume-title": "Advances in Optical and Electron Microscopy",
      "author": "G. Millonig",
      "year": "1968",
      "unstructured": "Millonig, G. &Marinozzi, V. (1968) Fixation and embedding in electron microscopy. InAdvances in Optical and Electron Microscopy (edited byBarer, R. &Cosslett, V.) Vol. 2. pp. 251?341. London: Academic Press."
    },
    {
      "key": "CR26",
      "first-page": "157",
      "volume": "143",
      "author": "C. Mittermayer",
      "year": "1971",
      "unstructured": "Mittermayer, C., Madreiter, H., Lederer, B. &Sandritter, W. (1971) Differential acid hydrolysis of euchromatin and heterochromatin.Beitr. Path. 143, 157?65.",
      "journal-title": "Beitr. Path."
    },
    {
      "key": "CR27",
      "doi-asserted-by": "crossref",
      "first-page": "293",
      "DOI": "10.1007/978-1-4684-0973-4_14",
      "volume-title": "Chromatin structure and function",
      "author": "C. Nicolini",
      "year": "1979",
      "unstructured": "Nicolini, C. (1979)Chromatin structure and function. Vol. A. pp. 293?322. New York: Plenum Press."
    },
    {
      "key": "CR28",
      "doi-asserted-by": "crossref",
      "first-page": "304",
      "DOI": "10.1083/jcb.59.2.304",
      "volume": "59",
      "author": "D. Olins",
      "year": "1973",
      "unstructured": "Olins, D. &Wright, E. (1973) Glutaraldehyde fixation of isolated eucaryotic nuclei.J. Cell Biol. 59, 304?17.",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "CR29",
      "unstructured": "Pellicciari, C., Manfredi Romanini, M. G., Casirola, G., Ippoliti, G. & Marini, G. (1976) Hydrolysis kinetics studies of the Feulgen reaction on different lymphocyte populations in man.Fifth Int. Congr. Histochem. Cytochem., Bucharest."
    },
    {
      "key": "CR30",
      "doi-asserted-by": "crossref",
      "first-page": "213",
      "DOI": "10.1007/BF01003306",
      "volume": "10",
      "author": "C. Pellicciari",
      "year": "1978",
      "unstructured": "Pellicciari, C. &Fraschini, A. (1978) Methods of denaturation and renaturation of DNA in interphasic chromatin: cytochemical quantitative analysis by Methyl Green staining.Histochem. J. 10, 213?22.",
      "journal-title": "Histochem. J."
    },
    {
      "key": "CR31",
      "doi-asserted-by": "crossref",
      "first-page": "1053",
      "DOI": "10.1177/21.12.1053",
      "volume": "21",
      "author": "R. Rasch",
      "year": "1973",
      "unstructured": "Rasch, R. &Rasch, E. (1973) Kinetics of hydrolysis during the Feulgen reaction for deoxyribonucleic acid. A re-evaluation.J. Histochem. Cytochem. 21, 1053?65.",
      "journal-title": "J. Histochem. Cytochem."
    },
    {
      "key": "CR32",
      "doi-asserted-by": "crossref",
      "first-page": "233",
      "DOI": "10.1016/0014-4827(77)90260-9",
      "volume": "106",
      "author": "A. Retief",
      "year": "1977",
      "unstructured": "Retief, A. &R\u00fcchel, R. (1977) Histones removed by fixation. Their role in the mechanism of chromosomal banding.Expl. Cell Res. 106, 233?7.",
      "journal-title": "Expl. Cell Res."
    },
    {
      "key": "CR33",
      "doi-asserted-by": "crossref",
      "first-page": "208",
      "DOI": "10.1083/jcb.17.1.208",
      "volume": "17",
      "author": "E. Reynolds",
      "year": "1963",
      "unstructured": "Reynolds, E. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.J. Cell Biol. 17, 208?13.",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "CR34",
      "first-page": "274",
      "volume": "142",
      "author": "D. Sabatini",
      "year": "1962",
      "unstructured": "Sabatini, D., Bensch, K. &Barrnett, R. (1962) New means of fixation for electron microscopy and histochemistry.Anat. Rec. 142, 274?90.",
      "journal-title": "Anat. Rec."
    },
    {
      "key": "CR35",
      "doi-asserted-by": "crossref",
      "first-page": "19",
      "DOI": "10.1083/jcb.17.1.19",
      "volume": "17",
      "author": "D. Sabatini",
      "year": "1963",
      "unstructured": "Sabatini, D., Bensch, K. &Barrnett, R. (1963) Cytochemistry and electron microscopy: the preservation of the cellular ultrastructure and enzymatic activity by aldehyde fixation.J. Cell Biol. 17, 19?58.",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "CR36",
      "doi-asserted-by": "crossref",
      "first-page": "57",
      "DOI": "10.1177/12.2.57",
      "volume": "12",
      "author": "D. Sabatini",
      "year": "1964",
      "unstructured": "Sabatini, D., Miller, F. &Barrnett, R. (1964) Aldehyde fixation for morphological and enzyme histochemical studies with the electron microscope.J. Histochem. Cytochem. 12, 57?71.",
      "journal-title": "J. Histochem. Cytochem."
    },
    {
      "key": "CR37",
      "doi-asserted-by": "crossref",
      "first-page": "30",
      "DOI": "10.1007/BF00281805",
      "volume": "9",
      "author": "J. Scott",
      "year": "1967",
      "unstructured": "Scott, J. (1967) On the mechanism of the Methyl Green-pyronine stain for nucleic acids.Histochemie 9, 30?46.",
      "journal-title": "Histochemie"
    },
    {
      "key": "CR38",
      "doi-asserted-by": "crossref",
      "first-page": "31",
      "DOI": "10.1016/S0022-2836(62)80114-4",
      "volume": "4",
      "author": "D. Stollar",
      "year": "1962",
      "unstructured": "Stollar, D. &Grossman, L. (1962) The reaction of formaldehyde with denatured DNA: spectrophotometric, immunologic and enzymic studies.J. molec. Biol. 4, 31?8.",
      "journal-title": "J. molec. Biol."
    },
    {
      "key": "CR39",
      "doi-asserted-by": "crossref",
      "first-page": "214",
      "DOI": "10.1016/0014-4827(73)90128-6",
      "volume": "81",
      "author": "A. Sumner",
      "year": "1973",
      "unstructured": "Sumner, A., Evans, H. &Buckland, R. (1973) Mechanism involved in the banding of chromosomes with Quinacrine and Giemsa. I. The effects of fixation in methanol-acetic acid.Expl. Cell Res. 81, 214?22.",
      "journal-title": "Expl. Cell Res."
    },
    {
      "key": "CR40",
      "first-page": "1245",
      "volume": "14",
      "author": "B. F. Trump",
      "year": "1965",
      "unstructured": "Trump, B. F. &Ericsson, J. L. (1965) The effect of the fixative solution on the ultrastructure of cells and tissues. A comparative analysis with particular attention to the proximal convoluted tubule of the rat kidney.Lab. Invest. 14, 1245?51.",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "CR41",
      "first-page": "368",
      "volume": "15",
      "author": "B. F. Trump",
      "year": "1966",
      "unstructured": "Trump, B. F. &Bulger, R. E. (1966) New ultrastructural characteristics of cells fixed in a glutaraldehyde-osmium tetroxide mixture.Lab. Invest. 15, 368?79.",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "CR42",
      "doi-asserted-by": "crossref",
      "first-page": "234",
      "DOI": "10.1177/9.3.234",
      "volume": "9",
      "author": "P. Duijn Van",
      "year": "1961",
      "unstructured": "Van Duijn, P. (1961) Acrolein-Schiff: a new staining method for proteins.J. Histochem. Cytochem. 9, 234?41.",
      "journal-title": "J. Histochem. Cytochem."
    },
    {
      "key": "CR43",
      "doi-asserted-by": "crossref",
      "first-page": "122",
      "DOI": "10.1016/0014-4827(69)90404-2",
      "volume": "56",
      "author": "A. Zetterberg",
      "year": "1969",
      "unstructured": "Zetterberg, A. &Auer, G. (1969) Early changes in the binding between DNA and histones in human leukocytes exposed to phytoemagglutinin.Exp. Cell Res. 56, 122?6.",
      "journal-title": "Exp. Cell Res."
    }
  ],
  "container-title": "The Histochemical Journal",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://link.springer.com/content/pdf/10.1007/BF01003288.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://link.springer.com/article/10.1007/BF01003288/fulltext.html",
      "content-type": "text/html",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://link.springer.com/content/pdf/10.1007/BF01003288",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        4
      ]
    ],
    "date-time": "2021-07-04T04:04:48Z",
    "timestamp": 1625371488000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://link.springer.com/10.1007/BF01003288"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1981,
        9
      ]
    ]
  },
  "references-count": 43,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1981,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "BF01003288"
  ],
  "URL": "http://dx.doi.org/10.1007/BF01003288",
  "relation": {},
  "ISSN": [
    "0018-2214",
    "1573-6865"
  ],
  "subject": [],
  "container-title-short": "Histochem J",
  "published": {
    "date-parts": [
      [
        1981,
        9
      ]
    ]
  }
}