DOI: 10.1007/bf00369579. Determination of specific monoclonal antibody secretion rate during very slow hybridoma growth

Determination of specific monoclonal antibody secretion rate during very slow hybridoma growth

10.1007/bf00369579

Crossref journal-article

Springer Science and Business Media LLC

Bioprocess Engineering (297)

Bibliography

Flickinger, M. C., Goebel, N. K., & Bohn, M. A. (1990). Determination of specific monoclonal antibody secretion rate during very slow hybridoma growth. Bioprocess Engineering, 5(4), 155â164.

Authors 3

M. C. Flickinger (first)
N. K. Goebel (additional)
M. A. Bohn (additional)

References 33 Referenced 36

Griffiths, J. B.: Overview of cell culture systems and their scale up. In: Spier, R. E.; Griffiths, J. B. (Eds.): Animal Cell Biotechnology, vol. 3, pp. 179?221. Academic Press 1988
Berg, G. J.; Bodeker, B. G. D.: Employing a ceramic matrix for the immobilization of mammalian cells in culture. In: Spier, R. E.; Griffiths, J. B. (Eds.): Animal Cell Biotechnology, vol. 3, pp. 322?336. Academic Press 1988
Tolbert, W. R.; Feder, J; Lewis, C. Jr.: Static culture maintenance system. U.S. Patent 4, 537, 860. August 27, 1985
Feder, J.: The development of large-scale animal cell perfusion culture systems. Adv. Biotechnol. Proc. 7 (1988) 125?152 / Adv. Biotechnol. Proc. by J. Feder (1988)
Takazawa, Y; Tokashiki, M.; Hamamoto, K.; Muratrami, H.: High cell density perfusion culture of hybridoma cells recycling high molecular weight components. Cytotechnol. 1 (1988) 171?178 (10.1007/BF00146818) / Cytotechnol. by Y Takazawa (1988)
Posillico, E. G.: Microencapsulation technology for large-scale antibody production. Bio/Technology 4 (1986) 114?117 / Bio/Technology by E. G. Posillico (1986)
Dean, R. C.; Karkare, S. B.; Ray, N. G.; Runstadler, P. W.; Venkatasubramanian, K.: Large scale culture of hybridoma and mammalian cells in fluidized bed bioreactors. In: M. MooYoung (Ed.): Bioreactor immobilized enzymes and cells fundamentals and applications, pp. 125?142. Elsevier Applied Science 1988
Runstadler, P. W.; Cernak, S. R.: Large-scale fluidized-bed, immobilized cultivation of animal cells at high densities. In: Spier, R. E.; Griffiths, J. B. (Eds.): Animal Cell Biotechnology, vol. 3, pp. 305?335. Academic Press 1988
Emery, N.: Growth of hybridomas and secretion of monoclonal antibodies in vitro. Presented at: Society of Chemical Industry Symposium on ?Large scale production of monoclonal antibodies.? London, Dec. 9, 1986
Reuveny, S.; Velez, D.; Miller, L.; Macmillan, J. D.: Comparison of cell propagation methods for their effect on monoclonal antibody yield in fermenters. J. Immunol. Method. 86 (1986) 61?69 (10.1016/0022-1759(86)90265-6) / J. Immunol. Method. by S. Reuveny (1986)
Miller, W. M.; Blanch, H. W.; Wilke, C. R.: Kinetic analysis of hybridoma growth in continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH. Biotechnol. Bioeng. 32 (1988) 945 (10.1002/bit.260320803) / Biotechnol. Bioeng. by W. M. Miller (1988)
Boraston, R.; Garland, S.; Birch, J. R.: Growth and antibody production by mouse hybridoma cells growing in oxygen-limited chemostat culture. J. Chem. Technol. Biotechnol. 336 (1984) 200 / J. Chem. Technol. Biotechnol. by R. Boraston (1984)
Boraston, R.; Thompson, P. W.; Garland, S.; Birch, J. R.: Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture. Dev. Biol. Stand. 55 (1984) 103?111 / Dev. Biol. Stand. by R. Boraston (1984)
Frame, K.: Kinetic analysis of hybridoma growth and monoclonal antibody production in a CSTR. Ph.D. Thesis Chemical Engineering, Univ. Minnesota, October, 1988
Cashel, M.; Rudd, K. E.: The stringent response. In: Neidhardt, F. C.; Ingraham, J. L.; Magasanik, B.; Low, K. B.; Schaechter, M.; Umbarger, H. E. (Eds.): Escherichia coli and Salmonella typhimurium cellular and molecular biology, vol. 2, pp. 1410?1438. Washington, D. C.: American Society Microbiology 1987 / Escherichia coli and Salmonella typhimurium cellular and molecular biology, vol. 2 by M. Cashel (1987)
Pirt, S. J.: Maintenance energy: a general model for energy-limited and energy-sufficient growth. Arch. Microbiol. 133 (1982) 300?302 (10.1007/BF00521294) / Arch. Microbiol. by S. J. Pirt (1982)
Stouthamer, A. H.; von Verseveld, H. W.: Microbial energetics should be considered in manipulating metabolism for biotechnological purposes. TIBTECH 5 (1987) 149?155 (10.1016/0167-7799(87)90010-2) / TIBTECH by A. H. Stouthamer (1987)
von Verseveld, H. W.; Arbige, M.; Chesbro, W. R.: Continuous culture of bacteria with biomass retention. TIBTECH 2 (1984) 8?12 (10.1016/0167-7799(84)90046-5) / TIBTECH by H. W. Verseveld von (1984)
Arbige, M.; Chesbro, W. R.: Very slow growth of Bacillus polymyxa: stringent response and maintenance energy. Arch. Microbiol. 132 (1982) 338?344 (10.1007/BF00413386) / Arch. Microbiol. by M. Arbige (1982)
Miller, W. M.; Wilke, C. R.; Blanch, H. W.: Transient responses of hybridoma metabolism to changes in oxygen supply rate in continuous culture. Biopr. Eng. 3 (1988) 103?111 (10.1007/BF00373473) / Biopr. Eng. by W. M. Miller (1988)
Miller, W. M.; Wilke, C. R.; Blanch, H. W: Transient responses of hybridoma cells to lactate and ammonia pulse and step changes in continuous culture. Biopr. Eng. 3 (1988) 113?122 (10.1007/BF00373474) / Biopr. Eng. by W. M. Miller (1988)
Burgess, T. L.; Kelly, R. B.: Constitutive and regulated secretion of proteins. Ann. Rev. Cell Biol. 3 (1987) 243?293 (10.1146/annurev.cb.03.110187.001331) / Ann. Rev. Cell Biol. by T. L. Burgess (1987)
Morgan, A. C.; Galloway, D. R.; Reisfeld, R. A.: Production and characterization of monoclonal antibody to a melanoma specific glycoprotein. Hybridoma 1 (1981) 27?36 (10.1089/hyb.1.1981.1.27) / Hybridoma by A. C. Morgan (1981)
In: Kruse, P. F.; Patterson, M. K. (Eds.): Tissue culture: methods and applications, pp. 406?408. Academic Press 1973
Jauregui, H. O.; Hagner, N. T.; Driscoll, J. L.; Williams-Hollard, R.; Lipsky, M. H.; Galletti, P. M.: Trypan blue dye uptake and lactate dehydrogenase in adult rat hepatocytes ? freshly isolated cells, cell suspensions, and primary monolayer cultures. In Vitro 17 (1981) 1100?1110 (10.1007/BF02618612) / In Vitro by H. O. Jauregui (1981)
Goebel, N. K.; Kuehn, R.; Flickinger, M. C.: Methods for determination of growth-rate-dependent changes in hybridoma volume, shape and surface structure during continuous recycle. Cytotechnol (1990) (in press) (10.1007/BF00148810)
Seaver, S. S.; Rudolph, J. L.; Gabriels, J. E.: A rapid HPLC technique for monitoring amino acid utilization in cell culture. Biotechniques (1984) 254?260
Lee, S. M.; Gustafson, M. E.; Pickle, D. J; Flickinger, M. C.; Muschik, G. M.; Morgan, A. C.: Large-scale purification of a murine antimelonoma monoclonal antibody. J. Biotechnol. 4 (1986) 189?204 (10.1016/0168-1656(86)90025-8) / J. Biotechnol. by S. M. Lee (1986)
Flickinger, M. C.; Lebherz, W. B.; Lee, S. M.; Pickle, D. J; Hopkins, R. F.: Process technologies for in vitro production of retrovirus and monoclonal antibody for use as anticancer biologics. Dev. Ind. Microbiol. 27 (1987) 87?99 / Dev. Ind. Microbiol. by M. C. Flickinger (1987)
Lehman, J; Vorlop, J; Buntemeyer, H.: Bubble-free reactors and their development for continuous culture with cell recycle. In: Spier, R. E.; Griffiths, J. B. (Eds.): Animal Cell Biotechnology, vol. 3, pp. 221?227, Academic Press 1988
Merten, O. W: Concentrating mammalian cells 1. large-scale animal cell culture. TIBTECH 5 (1987) 230?237 (10.1016/0167-7799(87)90053-9) / TIBTECH by O. W Merten (1987)
McQueen, A.; Meilhoc, E.; Bailey, J. E.: Flow effects on the viability and lysis of suspended mammalian cells. Biotechnol. Lett. 9 (1987) 831?836 (10.1007/BF01026191) / Biotechnol. Lett. by A. McQueen (1987)
Cowan, N. J.; Milstein, C.: Stability of cytoplasmic ribonucleic acid in a mouse myeloma: estimation of the half-life of the messenger RNA coding for an immunoglobulin light chain. J. Mol. Biol. 82 (1974) 469?481 (10.1016/0022-2836(74)90242-3) / J. Mol. Biol. by N. J. Cowan (1974)

Dates

Type	When
Created	20 years, 9 months ago (Nov. 9, 2004, 7:13 p.m.)
Deposited	6 years, 4 months ago (April 8, 2019, 5:43 p.m.)
Indexed	1 year, 9 months ago (Nov. 18, 2023, 6:33 a.m.)
Issued	35 years, 7 months ago (Jan. 1, 1990)
Published	35 years, 7 months ago (Jan. 1, 1990)
Published Print	35 years, 7 months ago (Jan. 1, 1990)

Funders 0

None

BibTeX

@article{Flickinger_1990, title={Determination of specific monoclonal antibody secretion rate during very slow hybridoma growth}, volume={5}, ISSN={1615-7605}, url={http://dx.doi.org/10.1007/bf00369579}, DOI={10.1007/bf00369579}, number={4}, journal={Bioprocess Engineering}, publisher={Springer Science and Business Media LLC}, author={Flickinger, M. C. and Goebel, N. K. and Bohn, M. A.}, year={1990}, pages={155–164} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        18
      ]
    ],
    "date-time": "2023-11-18T11:33:09Z",
    "timestamp": 1700307189234
  },
  "reference-count": 33,
  "publisher": "Springer Science and Business Media LLC",
  "issue": "4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1990,
            1,
            1
          ]
        ],
        "date-time": "1990-01-01T00:00:00Z",
        "timestamp": 631152000000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "http://www.springer.com/tdm"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1990
      ]
    ]
  },
  "DOI": "10.1007/bf00369579",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        11,
        10
      ]
    ],
    "date-time": "2004-11-10T00:13:36Z",
    "timestamp": 1100045616000
  },
  "page": "155-164",
  "source": "Crossref",
  "is-referenced-by-count": 36,
  "title": "Determination of specific monoclonal antibody secretion rate during very slow hybridoma growth",
  "prefix": "10.1007",
  "volume": "5",
  "author": [
    {
      "given": "M. C.",
      "family": "Flickinger",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "N. K.",
      "family": "Goebel",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M. A.",
      "family": "Bohn",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "297",
  "reference": [
    {
      "key": "CR1",
      "unstructured": "Griffiths, J. B.: Overview of cell culture systems and their scale up. In: Spier, R. E.; Griffiths, J. B. (Eds.): Animal Cell Biotechnology, vol. 3, pp. 179?221. Academic Press 1988"
    },
    {
      "key": "CR2",
      "unstructured": "Berg, G. J.; Bodeker, B. G. D.: Employing a ceramic matrix for the immobilization of mammalian cells in culture. In: Spier, R. E.; Griffiths, J. B. (Eds.): Animal Cell Biotechnology, vol. 3, pp. 322?336. Academic Press 1988"
    },
    {
      "key": "CR3",
      "unstructured": "Tolbert, W. R.; Feder, J; Lewis, C. Jr.: Static culture maintenance system. U.S. Patent 4, 537, 860. August 27, 1985"
    },
    {
      "key": "CR4",
      "first-page": "125",
      "volume": "7",
      "author": "J. Feder",
      "year": "1988",
      "unstructured": "Feder, J.: The development of large-scale animal cell perfusion culture systems. Adv. Biotechnol. Proc. 7 (1988) 125?152",
      "journal-title": "Adv. Biotechnol. Proc."
    },
    {
      "key": "CR5",
      "doi-asserted-by": "crossref",
      "first-page": "171",
      "DOI": "10.1007/BF00146818",
      "volume": "1",
      "author": "Y Takazawa",
      "year": "1988",
      "unstructured": "Takazawa, Y; Tokashiki, M.; Hamamoto, K.; Muratrami, H.: High cell density perfusion culture of hybridoma cells recycling high molecular weight components. Cytotechnol. 1 (1988) 171?178",
      "journal-title": "Cytotechnol."
    },
    {
      "key": "CR6",
      "first-page": "114",
      "volume": "4",
      "author": "E. G. Posillico",
      "year": "1986",
      "unstructured": "Posillico, E. G.: Microencapsulation technology for large-scale antibody production. Bio/Technology 4 (1986) 114?117",
      "journal-title": "Bio/Technology"
    },
    {
      "key": "CR7",
      "unstructured": "Dean, R. C.; Karkare, S. B.; Ray, N. G.; Runstadler, P. W.; Venkatasubramanian, K.: Large scale culture of hybridoma and mammalian cells in fluidized bed bioreactors. In: M. MooYoung (Ed.): Bioreactor immobilized enzymes and cells fundamentals and applications, pp. 125?142. Elsevier Applied Science 1988"
    },
    {
      "key": "CR8",
      "unstructured": "Runstadler, P. W.; Cernak, S. R.: Large-scale fluidized-bed, immobilized cultivation of animal cells at high densities. In: Spier, R. E.; Griffiths, J. B. (Eds.): Animal Cell Biotechnology, vol. 3, pp. 305?335. Academic Press 1988"
    },
    {
      "key": "CR9",
      "unstructured": "Emery, N.: Growth of hybridomas and secretion of monoclonal antibodies in vitro. Presented at: Society of Chemical Industry Symposium on ?Large scale production of monoclonal antibodies.? London, Dec. 9, 1986"
    },
    {
      "key": "CR10",
      "doi-asserted-by": "crossref",
      "first-page": "61",
      "DOI": "10.1016/0022-1759(86)90265-6",
      "volume": "86",
      "author": "S. Reuveny",
      "year": "1986",
      "unstructured": "Reuveny, S.; Velez, D.; Miller, L.; Macmillan, J. D.: Comparison of cell propagation methods for their effect on monoclonal antibody yield in fermenters. J. Immunol. Method. 86 (1986) 61?69",
      "journal-title": "J. Immunol. Method."
    },
    {
      "key": "CR11",
      "doi-asserted-by": "crossref",
      "first-page": "945",
      "DOI": "10.1002/bit.260320803",
      "volume": "32",
      "author": "W. M. Miller",
      "year": "1988",
      "unstructured": "Miller, W. M.; Blanch, H. W.; Wilke, C. R.: Kinetic analysis of hybridoma growth in continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH. Biotechnol. Bioeng. 32 (1988) 945",
      "journal-title": "Biotechnol. Bioeng."
    },
    {
      "key": "CR12",
      "first-page": "200",
      "volume": "336",
      "author": "R. Boraston",
      "year": "1984",
      "unstructured": "Boraston, R.; Garland, S.; Birch, J. R.: Growth and antibody production by mouse hybridoma cells growing in oxygen-limited chemostat culture. J. Chem. Technol. Biotechnol. 336 (1984) 200",
      "journal-title": "J. Chem. Technol. Biotechnol."
    },
    {
      "key": "CR13",
      "first-page": "103",
      "volume": "55",
      "author": "R. Boraston",
      "year": "1984",
      "unstructured": "Boraston, R.; Thompson, P. W.; Garland, S.; Birch, J. R.: Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture. Dev. Biol. Stand. 55 (1984) 103?111",
      "journal-title": "Dev. Biol. Stand."
    },
    {
      "key": "CR14",
      "unstructured": "Frame, K.: Kinetic analysis of hybridoma growth and monoclonal antibody production in a CSTR. Ph.D. Thesis Chemical Engineering, Univ. Minnesota, October, 1988"
    },
    {
      "key": "CR15",
      "first-page": "1410",
      "volume-title": "Escherichia coli and Salmonella typhimurium cellular and molecular biology, vol. 2",
      "author": "M. Cashel",
      "year": "1987",
      "unstructured": "Cashel, M.; Rudd, K. E.: The stringent response. In: Neidhardt, F. C.; Ingraham, J. L.; Magasanik, B.; Low, K. B.; Schaechter, M.; Umbarger, H. E. (Eds.): Escherichia coli and Salmonella typhimurium cellular and molecular biology, vol. 2, pp. 1410?1438. Washington, D. C.: American Society Microbiology 1987"
    },
    {
      "key": "CR16",
      "doi-asserted-by": "crossref",
      "first-page": "300",
      "DOI": "10.1007/BF00521294",
      "volume": "133",
      "author": "S. J. Pirt",
      "year": "1982",
      "unstructured": "Pirt, S. J.: Maintenance energy: a general model for energy-limited and energy-sufficient growth. Arch. Microbiol. 133 (1982) 300?302",
      "journal-title": "Arch. Microbiol."
    },
    {
      "key": "CR17",
      "doi-asserted-by": "crossref",
      "first-page": "149",
      "DOI": "10.1016/0167-7799(87)90010-2",
      "volume": "5",
      "author": "A. H. Stouthamer",
      "year": "1987",
      "unstructured": "Stouthamer, A. H.; von Verseveld, H. W.: Microbial energetics should be considered in manipulating metabolism for biotechnological purposes. TIBTECH 5 (1987) 149?155",
      "journal-title": "TIBTECH"
    },
    {
      "key": "CR18",
      "doi-asserted-by": "crossref",
      "first-page": "8",
      "DOI": "10.1016/0167-7799(84)90046-5",
      "volume": "2",
      "author": "H. W. Verseveld von",
      "year": "1984",
      "unstructured": "von Verseveld, H. W.; Arbige, M.; Chesbro, W. R.: Continuous culture of bacteria with biomass retention. TIBTECH 2 (1984) 8?12",
      "journal-title": "TIBTECH"
    },
    {
      "key": "CR19",
      "doi-asserted-by": "crossref",
      "first-page": "338",
      "DOI": "10.1007/BF00413386",
      "volume": "132",
      "author": "M. Arbige",
      "year": "1982",
      "unstructured": "Arbige, M.; Chesbro, W. R.: Very slow growth of Bacillus polymyxa: stringent response and maintenance energy. Arch. Microbiol. 132 (1982) 338?344",
      "journal-title": "Arch. Microbiol."
    },
    {
      "key": "CR20",
      "doi-asserted-by": "crossref",
      "first-page": "103",
      "DOI": "10.1007/BF00373473",
      "volume": "3",
      "author": "W. M. Miller",
      "year": "1988",
      "unstructured": "Miller, W. M.; Wilke, C. R.; Blanch, H. W.: Transient responses of hybridoma metabolism to changes in oxygen supply rate in continuous culture. Biopr. Eng. 3 (1988) 103?111",
      "journal-title": "Biopr. Eng."
    },
    {
      "key": "CR21",
      "doi-asserted-by": "crossref",
      "first-page": "113",
      "DOI": "10.1007/BF00373474",
      "volume": "3",
      "author": "W. M. Miller",
      "year": "1988",
      "unstructured": "Miller, W. M.; Wilke, C. R.; Blanch, H. W: Transient responses of hybridoma cells to lactate and ammonia pulse and step changes in continuous culture. Biopr. Eng. 3 (1988) 113?122",
      "journal-title": "Biopr. Eng."
    },
    {
      "key": "CR22",
      "doi-asserted-by": "crossref",
      "first-page": "243",
      "DOI": "10.1146/annurev.cb.03.110187.001331",
      "volume": "3",
      "author": "T. L. Burgess",
      "year": "1987",
      "unstructured": "Burgess, T. L.; Kelly, R. B.: Constitutive and regulated secretion of proteins. Ann. Rev. Cell Biol. 3 (1987) 243?293",
      "journal-title": "Ann. Rev. Cell Biol."
    },
    {
      "key": "CR23",
      "doi-asserted-by": "crossref",
      "first-page": "27",
      "DOI": "10.1089/hyb.1.1981.1.27",
      "volume": "1",
      "author": "A. C. Morgan",
      "year": "1981",
      "unstructured": "Morgan, A. C.; Galloway, D. R.; Reisfeld, R. A.: Production and characterization of monoclonal antibody to a melanoma specific glycoprotein. Hybridoma 1 (1981) 27?36",
      "journal-title": "Hybridoma"
    },
    {
      "key": "CR24",
      "unstructured": "In: Kruse, P. F.; Patterson, M. K. (Eds.): Tissue culture: methods and applications, pp. 406?408. Academic Press 1973"
    },
    {
      "key": "CR25",
      "doi-asserted-by": "crossref",
      "first-page": "1100",
      "DOI": "10.1007/BF02618612",
      "volume": "17",
      "author": "H. O. Jauregui",
      "year": "1981",
      "unstructured": "Jauregui, H. O.; Hagner, N. T.; Driscoll, J. L.; Williams-Hollard, R.; Lipsky, M. H.; Galletti, P. M.: Trypan blue dye uptake and lactate dehydrogenase in adult rat hepatocytes ? freshly isolated cells, cell suspensions, and primary monolayer cultures. In Vitro 17 (1981) 1100?1110",
      "journal-title": "In Vitro"
    },
    {
      "key": "CR26",
      "doi-asserted-by": "crossref",
      "unstructured": "Goebel, N. K.; Kuehn, R.; Flickinger, M. C.: Methods for determination of growth-rate-dependent changes in hybridoma volume, shape and surface structure during continuous recycle. Cytotechnol (1990) (in press)",
      "DOI": "10.1007/BF00148810"
    },
    {
      "key": "CR27",
      "unstructured": "Seaver, S. S.; Rudolph, J. L.; Gabriels, J. E.: A rapid HPLC technique for monitoring amino acid utilization in cell culture. Biotechniques (1984) 254?260"
    },
    {
      "key": "CR28",
      "doi-asserted-by": "crossref",
      "first-page": "189",
      "DOI": "10.1016/0168-1656(86)90025-8",
      "volume": "4",
      "author": "S. M. Lee",
      "year": "1986",
      "unstructured": "Lee, S. M.; Gustafson, M. E.; Pickle, D. J; Flickinger, M. C.; Muschik, G. M.; Morgan, A. C.: Large-scale purification of a murine antimelonoma monoclonal antibody. J. Biotechnol. 4 (1986) 189?204",
      "journal-title": "J. Biotechnol."
    },
    {
      "key": "CR29",
      "first-page": "87",
      "volume": "27",
      "author": "M. C. Flickinger",
      "year": "1987",
      "unstructured": "Flickinger, M. C.; Lebherz, W. B.; Lee, S. M.; Pickle, D. J; Hopkins, R. F.: Process technologies for in vitro production of retrovirus and monoclonal antibody for use as anticancer biologics. Dev. Ind. Microbiol. 27 (1987) 87?99",
      "journal-title": "Dev. Ind. Microbiol."
    },
    {
      "key": "CR30",
      "unstructured": "Lehman, J; Vorlop, J; Buntemeyer, H.: Bubble-free reactors and their development for continuous culture with cell recycle. In: Spier, R. E.; Griffiths, J. B. (Eds.): Animal Cell Biotechnology, vol. 3, pp. 221?227, Academic Press 1988"
    },
    {
      "key": "CR31",
      "doi-asserted-by": "crossref",
      "first-page": "230",
      "DOI": "10.1016/0167-7799(87)90053-9",
      "volume": "5",
      "author": "O. W Merten",
      "year": "1987",
      "unstructured": "Merten, O. W: Concentrating mammalian cells 1. large-scale animal cell culture. TIBTECH 5 (1987) 230?237",
      "journal-title": "TIBTECH"
    },
    {
      "key": "CR32",
      "doi-asserted-by": "crossref",
      "first-page": "831",
      "DOI": "10.1007/BF01026191",
      "volume": "9",
      "author": "A. McQueen",
      "year": "1987",
      "unstructured": "McQueen, A.; Meilhoc, E.; Bailey, J. E.: Flow effects on the viability and lysis of suspended mammalian cells. Biotechnol. Lett. 9 (1987) 831?836",
      "journal-title": "Biotechnol. Lett."
    },
    {
      "key": "CR33",
      "doi-asserted-by": "crossref",
      "first-page": "469",
      "DOI": "10.1016/0022-2836(74)90242-3",
      "volume": "82",
      "author": "N. J. Cowan",
      "year": "1974",
      "unstructured": "Cowan, N. J.; Milstein, C.: Stability of cytoplasmic ribonucleic acid in a mouse myeloma: estimation of the half-life of the messenger RNA coding for an immunoglobulin light chain. J. Mol. Biol. 82 (1974) 469?481",
      "journal-title": "J. Mol. Biol."
    }
  ],
  "container-title": "Bioprocess Engineering",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://link.springer.com/content/pdf/10.1007/BF00369579.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://link.springer.com/article/10.1007/BF00369579/fulltext.html",
      "content-type": "text/html",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://link.springer.com/content/pdf/10.1007/BF00369579",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2019,
        4,
        8
      ]
    ],
    "date-time": "2019-04-08T21:43:48Z",
    "timestamp": 1554759828000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://link.springer.com/10.1007/BF00369579"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1990
      ]
    ]
  },
  "references-count": 33,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1990
        ]
      ]
    }
  },
  "alternative-id": [
    "BF00369579"
  ],
  "URL": "http://dx.doi.org/10.1007/BF00369579",
  "relation": {},
  "ISSN": [
    "0178-515X",
    "1615-7605"
  ],
  "subject": [],
  "container-title-short": "Bioprocess Engineering",
  "published": {
    "date-parts": [
      [
        1990
      ]
    ]
  }
}