DOI: 10.1007/bf00267823. A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes

A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes

10.1007/bf00267823

Crossref journal-article

Springer Science and Business Media LLC

Histochemistry (297)

Bibliography

Schaeren-Wiemers, N., & Gerfin-Moser, A. (1993). A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes. Histochemistry, 100(6), 431â440.

Authors 2

Nicole Schaeren-Wiemers (first)
Andrea Gerfin-Moser (additional)

References 21 Referenced 1,005

Angerer LM, Angerer RC (1991) Localization of mRNAs by in situ hybridization. Methods Cell Biol 35:37?71 (10.1016/S0091-679X(08)60568-3) / Methods Cell Biol by LM Angerer (1991)
Bachmann S, Le Hir M, Eckardt K-U (1993) Co-localization of erythropoietin mRNA and ecto-5?-nucleotidase immunoreactivity in peritubular cells of rat renal cortex indicates that fibroblasts produce erythropoietin. J Histochem Cytochem 41:335?341 (10.1177/41.3.8429197) / J Histochem Cytochem by S Bachmann (1993)
Berridge MJ, Irvine RF (1989) Inositol phosphates and cell signalling. Nature 341:197?205 (10.1038/341197a0) / Nature by MJ Berridge (1989)
Biffo S, Verdun di Cantogno L, Fasolo A (1992) Double labeling with non-isotopic in situ hybridization and BrdU immunohistochemistry: calmodulin (CaM) mRNA expression in postmitotic neurons of the olfactory system. J Histochem Cytochem 40:535?540 (10.1177/40.4.1552187) / J Histochem Cytochem by S Biffo (1992)
Boehringer Mannheim (1992) Nonradioactive in situ hybridization: application manual. Boehringer Mannheim GmbH Biochemica, Mannheim, Germany / Nonradioactive in situ hybridization: application manual. Boehringer Mannheim GmbH Biochemica by Boehringer Mannheim (1992)
Colman DR, Kreibich G, Frey AB, Sabatini DD (1982) Synthesis and incorporation of myelin peptides into CNS myelin. J Cell Biol 95:598?608 (10.1083/jcb.95.2.598) / J Cell Biol by DR Colman (1982)
D�rries U, Bartsch U, Nolte Ch, Roth J, Schachner M (1993) Adaptation of a non-radioactive in situ hybridization method to electron microscopy: detection of tenascin mRNAs in mouse cerebellum with digoxigenin-labelled probes and gold-labelled antibodies. Histochemistry 99:251?262 (10.1007/BF00269143) / Histochemistry by U D�rries (1993)
Emson PC (1993) In-situ hybridization as a methodological tool for the neuroscientist. Trends Neurol Sci 16:9?16 (10.1016/0166-2236(93)90041-J) / Trends Neurol Sci by PC Emson (1993)
Hollmann M, O'Shea-Greenfield A, Rogers SW, Heinemann S (1989) Cloning by functional expression of a member of the glutamate receptor family. Nature 342:643?648 (10.1038/342643a0) / Nature by M Hollmann (1989)
Keinaenen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556?560 (10.1126/science.2166337) / Science by K Keinaenen (1990)
Komminoth P, Merk FB, Leav I, Wolfe HJ, Roth J (1992) Comparison of 35S- and digoxigenin-labelled RNA and oligonucleotide probes for in situ hybridization. Histochemistry 98:217?228 (10.1007/BF00271035) / Histochemistry by P Komminoth (1992)
Lyons GE, Schiaffino S, Sassoon D, Barton P, Buckingham M (1990) Developmental regulation of myosin gene expression in mouse cardiac muscle. J Cell Biol 111:2427?2436 (10.1083/jcb.111.6.2427) / J Cell Biol by GE Lyons (1990)
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a labatory manual. Cold Spring Harbour Laboratory, Cold Spring Harbor, NY / Molecular cloning, a labatory manual by T Maniatis (1982)
Oh JD, Woolf NJ, Roghani A, Edwards RH, Butcher LL (1992) Cholinergic neurons in the rat central nervous system demonstrated by in situ hybridization of choline acetyltransferase mRNA. Neuroscience 47:807?822 (10.1016/0306-4522(92)90031-V) / Neuroscience by JD Oh (1992)
Ohtani H, Kuroiwa A, Obinata M, Ooshima A, Nagura H (1992) Identification of type I collagen-producing cells in human gastrointestinal carcinomas by non-radioactive in situ hybridization and immunoelectron microscopy. J Histochem Cytochem 40:1139?1146 (10.1177/40.8.1619278) / J Histochem Cytochem by H Ohtani (1992)
Raff MC, Miller RH, Noble M (1983) A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature 303:390?396 (10.1038/303390a0) / Nature by MC Raff (1983)
Ross TS, Jefferson AB, Mitchell CA, Majerus PW (1991) Cloning and expression of human 75-kDa inositol polyphosphate-5-phosphatase. J Biol Chem 266:20283?20289 (10.1016/S0021-9258(18)54920-6) / J Biol Chem by TS Ross (1991)
Schaeren-Wiemers N, Schaefer C, Yancopoulos GD, Schwab ME (1991) A differential screening approach to isolate new oligodendrocyte-specific cDNA clones. Soc Neurosci Abstr 17:1149 / Soc Neurosci Abstr by N Schaeren-Wiemers (1991)
Springer JE, Robbins E, Gwag BJ, Lewis ME, Baldino F Jr (1991) Non-radioactive detection of nerve growth factor receptor (NGFR) mRNA in rat brain using in situ hybridization histochemistry. J Histochem Cytochem 39:231?234 (10.1177/39.2.1846159) / J Histochem Cytochem by JE Springer (1991)
Verity AN, Levine MS, Campagnoni AT (1990) Gene expression in the jimpy mutant: evidence for fewer oligodendrocytes expressing myelin protein genes and impaired translocation of myelin basic protein mRNA. Dev Neurosci 12:359?372 (10.1159/000111864) / Dev Neurosci by AN Verity (1990)
Wahle P, Beckh S (1992) A method of in situ hybridization combined with immunocytochemistry, histochemistry, and tract tracing to characterize the mRNA expressing cell types in heterogenous neuronal populations. J Neurosci Methods 41:153?166 (10.1016/0165-0270(92)90057-K) / J Neurosci Methods by P Wahle (1992)

Dates

Type	When
Created	20 years, 11 months ago (Sept. 29, 2004, 12:44 a.m.)
Deposited	4 years, 2 months ago (June 25, 2021, 9:18 p.m.)
Indexed	4 weeks ago (Aug. 3, 2025, 7 p.m.)
Issued	31 years, 9 months ago (Dec. 1, 1993)
Published	31 years, 9 months ago (Dec. 1, 1993)
Published Print	31 years, 9 months ago (Dec. 1, 1993)

Funders 0

None

BibTeX

@article{Schaeren_Wiemers_1993, title={A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes}, volume={100}, ISSN={1432-119X}, url={http://dx.doi.org/10.1007/bf00267823}, DOI={10.1007/bf00267823}, number={6}, journal={Histochemistry}, publisher={Springer Science and Business Media LLC}, author={Schaeren-Wiemers, Nicole and Gerfin-Moser, Andrea}, year={1993}, month=dec, pages={431–440} }

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