DOI: 10.1007/bf00014912. Import into chloroplasts of a yeast mitochondrial protein directed by ferredoxin and plastocyanin transit peptides

Import into chloroplasts of a yeast mitochondrial protein directed by ferredoxin and plastocyanin transit peptides

10.1007/bf00014912

Crossref journal-article

Springer Science and Business Media LLC

Plant Molecular Biology (297)

Bibliography

Smeekens, S., van Steeg, H., Bauerle, C., Bettenbroek, H., Keegstra, K., & Weisbeek, P. (1987). Import into chloroplasts of a yeast mitochondrial protein directed by ferredoxin and plastocyanin transit peptides. Plant Molecular Biology, 9(4), 377â388.

Authors 6

Sjef Smeekens (first)
Harry van Steeg (additional)
Cynthia Bauerle (additional)
Harry Bettenbroek (additional)
Kenneth Keegstra (additional)
Peter Weisbeek (additional)

References 30 Referenced 57

Cashmore A, Szabo L, Timko M, Kausch A, Van denBroeck G, Schreier P, Bohnert H, Herrera-Estrella L, VanMontagu M, Schell J: Import of polypeptides into chloroplasts. Bio/Technology 3: 803?808 (1985). / Bio/Technology by A Cashmore (1985)
Chamberlain J: Fluorographic detection of radioactivity in polyacrylamide gels with the water soluble fluor, sodium salicylate. Anal Biochem 98: 132?135 (1979). (10.1016/0003-2697(79)90716-4) / Anal Biochem by J Chamberlain (1979)
Cline K, Werner-Washburne M, Andrews J, Keegstra K: Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts. Plant Physiol 75: 675?678 (1984). (10.1104/pp.75.3.675) / Plant Physiol by K Cline (1984)
Cline K, Werner-Washburne M, Lubben T, Keegstra K: Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts. J Biol Chem 260: 3691?3696 (1985). (10.1016/S0021-9258(19)83678-5) / J Biol Chem by K Cline (1985)
Ellis JR: Chloroplast proteins: synthesis, transport and assembly. Ann Rev Plant Physiol 32: 111?137 (1981). (10.1146/annurev.pp.32.060181.000551) / Ann Rev Plant Physiol by JR Ellis (1981)
Emr S, Vassarotti A, Garrett J, Geller B, Takeda M, Douglas M: The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal. J Cell Biol 102: 523?533 (1986). (10.1083/jcb.102.2.523) / J Cell Biol by S Emr (1986)
Gasser S, Daum G, Schatz G: Import of proteins into mitochondria. Energy dependent uptake of precursors by isolated mitochondria. J Biol Chem 257: 13034?13041 (1982). (10.1016/S0021-9258(18)33618-4) / J Biol Chem by S Gasser (1982)
Hageman J, Robinson C, Smeekens S, Weisbeek P: A thylakoid processing protease required for complete maturation of the lumen protein plastocyanin. Nature 324: 567?569 (1986). (10.1038/324567a0) / Nature by J Hageman (1986)
Hay R, Bohni P, Gasser S: How mitochondria import proteins. Biochim Biophys Acta 779: 65?87 (1984). (10.1016/0304-4157(84)90004-2) / Biochim Biophys Acta by R Hay (1984)
Horwich A, Kalousek F, Mellman I, Rosenberg L: A leader peptide is sufficient to direct mitochondrial import of a chimeric protein. EMBO J 4: 1129?1135 (1985). (10.1002/j.1460-2075.1985.tb03750.x) / EMBO J by A Horwich (1985)
Hurt E, Pesold-Hurt B, Suda K, Opligger W, Schatz G: The first twelve amino acids (less than half of the presequence) of an imported mitochondrial protein can direct mouse cytosolic dihydropholate reductase into the yeast mitochondrial matrix. EMBO J 4: 2061?2068 (1985). (10.1002/j.1460-2075.1985.tb03892.x) / EMBO J by E Hurt (1985)
Hurt E, Soltanifar N, Goldschmidt-Clermont M, Rochaix J-D, Schatz G: The cleavable presequence of an imported chloroplast protein directs attached polypeptides into yeast mitochondria. EMBO J 5: 1343?1350 (1986). (10.1002/j.1460-2075.1986.tb04365.x) / EMBO J by E Hurt (1986)
Keng T, Alani E, Guarente L: The nine amino-terminal residues of delta-aminolevulinate synthase direct betagalactosidase into the mitochondrial matrix. Mol Cell Biol 6: 355?364 (1986). (10.1128/MCB.6.2.355) / Mol Cell Biol by T Keng (1986)
Laemmli U: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227: 680?685 (1970). (10.1038/227680a0) / Nature by U Laemmli (1970)
Laskey R, Mills A: Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem 56: 335?341 (1975). (10.1111/j.1432-1033.1975.tb02238.x) / Eur J Biochem by R Laskey (1975)
Lubben T, Keegstra K: Efficient in vitro import of a cytosolic heat shock protein into pea chloroplasts. Proc Natl Acad Sci USA 83: 5502?5506 (1986). (10.1073/pnas.83.15.5502) / Proc Natl Acad Sci USA by T Lubben (1986)
Maniatis T, Fritsch E, Sambrook J: Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory (1982). / Molecular Cloning. A Laboratory Manual by T Maniatis (1982)
Marres C, VanLoon A, Oudshoorn P, VanSteeg H, Grivell L, Slater E: Nucleotide sequence analysis of the nuclear gene coding for manganese superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron-sulphur protein. Eur J Biochem 147: 153?161 (1985). (10.1111/j.1432-1033.1985.tb08731.x) / Eur J Biochem by C Marres (1985)
Melton D, Krieg P, Rebagliati M, Maniatis T, Zinn K, Green M: Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucl Acids Res 12: 7035?7056 (1984). (10.1093/nar/12.18.7035) / Nucl Acids Res by D Melton (1984)
Pelham H, Jackson R: An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem 67: 247?256 (1976). (10.1111/j.1432-1033.1976.tb10656.x) / Eur J Biochem by H Pelham (1976)
Schmidt GW, Mishkind M: The transport of proteins into chloroplasts. Ann Rev Biochem 55: 879?912 (1986). (10.1146/annurev.bi.55.070186.004311) / Ann Rev Biochem by GW Schmidt (1986)
Schreier PH, Seftor EA, Schell J, Bohnert HJ: The use of nuclear encoded sequences to direct the light regulated synthesis and transport of a foreign protein into plant chloroplasts. EMBO J 4: 25?32 (1985). (10.1002/j.1460-2075.1985.tb02312.x) / EMBO J by PH Schreier (1985)
Smeekens S, VanBinsbergen J, Weisbeek P: The plant ferredoxin precursor: nucleotide sequence of a full length cDNA clone. Nucl Acids Res 13: 3179?3194 (1985). (10.1093/nar/13.9.3179) / Nucl Acids Res by S Smeekens (1985)
Smeekens S, DeGroot M, VanBinsbergen J, Weisbeek P: Sequence of the precursor of the chloroplast lumen protein plastocyanin. Nature 317: 456?458 (1985). (10.1038/317456a0) / Nature by S Smeekens (1985)
Smeekens S, Bauerle C, Hageman J, Keegstra K, Weisbeek P: The role of the transit peptide in the routing of precursors toward different chloroplast compartments. Cell 46: 365?375 (1986). (10.1016/0092-8674(86)90657-4) / Cell by S Smeekens (1986)
Steuber D, Ibrahimi I, Cutler D, Dobberstein B, Bujard H: A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences. EMBO J 3: 3143?3148 (1984). (10.1002/j.1460-2075.1984.tb02271.x) / EMBO J by D Steuber (1984)
Van denBroeck G, Timko MP, Kausch AP, Cashmore AR, VanMontagu M, Herrera-Estrella L: Targeting of foreign proteins to chloroplasts by fusion to the transit peptide from the small subunit of ribulose-1,5-bisphosphate carboxylase. Nature 313: 358?363 (1985). (10.1038/313358a0) / Nature by G Broeck Van den (1985)
VanLoon A, Brandli A, Schatz G: The presequences of two imported mitochondrial proteins contain information for intracellular and intramitochondrial sorting. Cell 44: 801?812 (1986). (10.1016/0092-8674(86)90846-9) / Cell by A Loon Van (1986)
VanSteeg H, Oudshoorn P, VanHell B, Polman J, Grivell LA: Targeting efficiency of a mitochondrial pre-sequence is dependent on the passenger protein. EMBO J 5: 3643?3650 (1986). (10.1002/j.1460-2075.1986.tb04694.x) / EMBO J by H Steeg Van (1986)
Wasmann C, Reis B, Bartlett S, Bohnert H: The importance of the transit peptide and the transported protein for protein import into chloroplasts. Mol Gen Genet 205: 446?453 (1986). (10.1007/BF00338081) / Mol Gen Genet by C Wasmann (1986)

Dates

Type	When
Created	20 years, 8 months ago (Dec. 21, 2004, 10:21 a.m.)
Deposited	4 years, 1 month ago (July 1, 2021, 8:13 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 5, 2025, 8:53 a.m.)
Issued	38 years, 1 month ago (July 1, 1987)
Published	38 years, 1 month ago (July 1, 1987)
Published Print	38 years, 1 month ago (July 1, 1987)

Funders 0

None

BibTeX

@article{Smeekens_1987, title={Import into chloroplasts of a yeast mitochondrial protein directed by ferredoxin and plastocyanin transit peptides}, volume={9}, ISSN={1573-5028}, url={http://dx.doi.org/10.1007/bf00014912}, DOI={10.1007/bf00014912}, number={4}, journal={Plant Molecular Biology}, publisher={Springer Science and Business Media LLC}, author={Smeekens, Sjef and van Steeg, Harry and Bauerle, Cynthia and Bettenbroek, Harry and Keegstra, Kenneth and Weisbeek, Peter}, year={1987}, month=jul, pages={377–388} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T12:53:24Z",
    "timestamp": 1754398404071
  },
  "reference-count": 30,
  "publisher": "Springer Science and Business Media LLC",
  "issue": "4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1987,
            7,
            1
          ]
        ],
        "date-time": "1987-07-01T00:00:00Z",
        "timestamp": 552096000000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "http://www.springer.com/tdm"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1987,
        7
      ]
    ]
  },
  "DOI": "10.1007/bf00014912",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        12,
        21
      ]
    ],
    "date-time": "2004-12-21T15:21:20Z",
    "timestamp": 1103642480000
  },
  "page": "377-388",
  "source": "Crossref",
  "is-referenced-by-count": 57,
  "title": "Import into chloroplasts of a yeast mitochondrial protein directed by ferredoxin and plastocyanin transit peptides",
  "prefix": "10.1007",
  "volume": "9",
  "author": [
    {
      "given": "Sjef",
      "family": "Smeekens",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Harry",
      "family": "van Steeg",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Cynthia",
      "family": "Bauerle",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Harry",
      "family": "Bettenbroek",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Kenneth",
      "family": "Keegstra",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Peter",
      "family": "Weisbeek",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "297",
  "reference": [
    {
      "key": "CR1",
      "first-page": "803",
      "volume": "3",
      "author": "A Cashmore",
      "year": "1985",
      "unstructured": "Cashmore A, Szabo L, Timko M, Kausch A, Van denBroeck G, Schreier P, Bohnert H, Herrera-Estrella L, VanMontagu M, Schell J: Import of polypeptides into chloroplasts. Bio/Technology 3: 803?808 (1985).",
      "journal-title": "Bio/Technology"
    },
    {
      "key": "CR2",
      "doi-asserted-by": "crossref",
      "first-page": "132",
      "DOI": "10.1016/0003-2697(79)90716-4",
      "volume": "98",
      "author": "J Chamberlain",
      "year": "1979",
      "unstructured": "Chamberlain J: Fluorographic detection of radioactivity in polyacrylamide gels with the water soluble fluor, sodium salicylate. Anal Biochem 98: 132?135 (1979).",
      "journal-title": "Anal Biochem"
    },
    {
      "key": "CR3",
      "doi-asserted-by": "crossref",
      "first-page": "675",
      "DOI": "10.1104/pp.75.3.675",
      "volume": "75",
      "author": "K Cline",
      "year": "1984",
      "unstructured": "Cline K, Werner-Washburne M, Andrews J, Keegstra K: Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts. Plant Physiol 75: 675?678 (1984).",
      "journal-title": "Plant Physiol"
    },
    {
      "key": "CR4",
      "doi-asserted-by": "crossref",
      "first-page": "3691",
      "DOI": "10.1016/S0021-9258(19)83678-5",
      "volume": "260",
      "author": "K Cline",
      "year": "1985",
      "unstructured": "Cline K, Werner-Washburne M, Lubben T, Keegstra K: Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts. J Biol Chem 260: 3691?3696 (1985).",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "CR5",
      "doi-asserted-by": "crossref",
      "first-page": "111",
      "DOI": "10.1146/annurev.pp.32.060181.000551",
      "volume": "32",
      "author": "JR Ellis",
      "year": "1981",
      "unstructured": "Ellis JR: Chloroplast proteins: synthesis, transport and assembly. Ann Rev Plant Physiol 32: 111?137 (1981).",
      "journal-title": "Ann Rev Plant Physiol"
    },
    {
      "key": "CR6",
      "doi-asserted-by": "crossref",
      "first-page": "523",
      "DOI": "10.1083/jcb.102.2.523",
      "volume": "102",
      "author": "S Emr",
      "year": "1986",
      "unstructured": "Emr S, Vassarotti A, Garrett J, Geller B, Takeda M, Douglas M: The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal. J Cell Biol 102: 523?533 (1986).",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "CR7",
      "doi-asserted-by": "crossref",
      "first-page": "13034",
      "DOI": "10.1016/S0021-9258(18)33618-4",
      "volume": "257",
      "author": "S Gasser",
      "year": "1982",
      "unstructured": "Gasser S, Daum G, Schatz G: Import of proteins into mitochondria. Energy dependent uptake of precursors by isolated mitochondria. J Biol Chem 257: 13034?13041 (1982).",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "CR8",
      "doi-asserted-by": "crossref",
      "first-page": "567",
      "DOI": "10.1038/324567a0",
      "volume": "324",
      "author": "J Hageman",
      "year": "1986",
      "unstructured": "Hageman J, Robinson C, Smeekens S, Weisbeek P: A thylakoid processing protease required for complete maturation of the lumen protein plastocyanin. Nature 324: 567?569 (1986).",
      "journal-title": "Nature"
    },
    {
      "key": "CR9",
      "doi-asserted-by": "crossref",
      "first-page": "65",
      "DOI": "10.1016/0304-4157(84)90004-2",
      "volume": "779",
      "author": "R Hay",
      "year": "1984",
      "unstructured": "Hay R, Bohni P, Gasser S: How mitochondria import proteins. Biochim Biophys Acta 779: 65?87 (1984).",
      "journal-title": "Biochim Biophys Acta"
    },
    {
      "key": "CR10",
      "doi-asserted-by": "crossref",
      "first-page": "1129",
      "DOI": "10.1002/j.1460-2075.1985.tb03750.x",
      "volume": "4",
      "author": "A Horwich",
      "year": "1985",
      "unstructured": "Horwich A, Kalousek F, Mellman I, Rosenberg L: A leader peptide is sufficient to direct mitochondrial import of a chimeric protein. EMBO J 4: 1129?1135 (1985).",
      "journal-title": "EMBO J"
    },
    {
      "key": "CR11",
      "doi-asserted-by": "crossref",
      "first-page": "2061",
      "DOI": "10.1002/j.1460-2075.1985.tb03892.x",
      "volume": "4",
      "author": "E Hurt",
      "year": "1985",
      "unstructured": "Hurt E, Pesold-Hurt B, Suda K, Opligger W, Schatz G: The first twelve amino acids (less than half of the presequence) of an imported mitochondrial protein can direct mouse cytosolic dihydropholate reductase into the yeast mitochondrial matrix. EMBO J 4: 2061?2068 (1985).",
      "journal-title": "EMBO J"
    },
    {
      "key": "CR12",
      "doi-asserted-by": "crossref",
      "first-page": "1343",
      "DOI": "10.1002/j.1460-2075.1986.tb04365.x",
      "volume": "5",
      "author": "E Hurt",
      "year": "1986",
      "unstructured": "Hurt E, Soltanifar N, Goldschmidt-Clermont M, Rochaix J-D, Schatz G: The cleavable presequence of an imported chloroplast protein directs attached polypeptides into yeast mitochondria. EMBO J 5: 1343?1350 (1986).",
      "journal-title": "EMBO J"
    },
    {
      "key": "CR13",
      "doi-asserted-by": "crossref",
      "first-page": "355",
      "DOI": "10.1128/MCB.6.2.355",
      "volume": "6",
      "author": "T Keng",
      "year": "1986",
      "unstructured": "Keng T, Alani E, Guarente L: The nine amino-terminal residues of delta-aminolevulinate synthase direct betagalactosidase into the mitochondrial matrix. Mol Cell Biol 6: 355?364 (1986).",
      "journal-title": "Mol Cell Biol"
    },
    {
      "key": "CR14",
      "doi-asserted-by": "crossref",
      "first-page": "680",
      "DOI": "10.1038/227680a0",
      "volume": "227",
      "author": "U Laemmli",
      "year": "1970",
      "unstructured": "Laemmli U: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227: 680?685 (1970).",
      "journal-title": "Nature"
    },
    {
      "key": "CR15",
      "doi-asserted-by": "crossref",
      "first-page": "335",
      "DOI": "10.1111/j.1432-1033.1975.tb02238.x",
      "volume": "56",
      "author": "R Laskey",
      "year": "1975",
      "unstructured": "Laskey R, Mills A: Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem 56: 335?341 (1975).",
      "journal-title": "Eur J Biochem"
    },
    {
      "key": "CR16",
      "doi-asserted-by": "crossref",
      "first-page": "5502",
      "DOI": "10.1073/pnas.83.15.5502",
      "volume": "83",
      "author": "T Lubben",
      "year": "1986",
      "unstructured": "Lubben T, Keegstra K: Efficient in vitro import of a cytosolic heat shock protein into pea chloroplasts. Proc Natl Acad Sci USA 83: 5502?5506 (1986).",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "CR17",
      "volume-title": "Molecular Cloning. A Laboratory Manual",
      "author": "T Maniatis",
      "year": "1982",
      "unstructured": "Maniatis T, Fritsch E, Sambrook J: Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory (1982)."
    },
    {
      "key": "CR18",
      "doi-asserted-by": "crossref",
      "first-page": "153",
      "DOI": "10.1111/j.1432-1033.1985.tb08731.x",
      "volume": "147",
      "author": "C Marres",
      "year": "1985",
      "unstructured": "Marres C, VanLoon A, Oudshoorn P, VanSteeg H, Grivell L, Slater E: Nucleotide sequence analysis of the nuclear gene coding for manganese superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron-sulphur protein. Eur J Biochem 147: 153?161 (1985).",
      "journal-title": "Eur J Biochem"
    },
    {
      "key": "CR19",
      "doi-asserted-by": "crossref",
      "first-page": "7035",
      "DOI": "10.1093/nar/12.18.7035",
      "volume": "12",
      "author": "D Melton",
      "year": "1984",
      "unstructured": "Melton D, Krieg P, Rebagliati M, Maniatis T, Zinn K, Green M: Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucl Acids Res 12: 7035?7056 (1984).",
      "journal-title": "Nucl Acids Res"
    },
    {
      "key": "CR20",
      "doi-asserted-by": "crossref",
      "first-page": "247",
      "DOI": "10.1111/j.1432-1033.1976.tb10656.x",
      "volume": "67",
      "author": "H Pelham",
      "year": "1976",
      "unstructured": "Pelham H, Jackson R: An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem 67: 247?256 (1976).",
      "journal-title": "Eur J Biochem"
    },
    {
      "key": "CR21",
      "doi-asserted-by": "crossref",
      "first-page": "879",
      "DOI": "10.1146/annurev.bi.55.070186.004311",
      "volume": "55",
      "author": "GW Schmidt",
      "year": "1986",
      "unstructured": "Schmidt GW, Mishkind M: The transport of proteins into chloroplasts. Ann Rev Biochem 55: 879?912 (1986).",
      "journal-title": "Ann Rev Biochem"
    },
    {
      "key": "CR22",
      "doi-asserted-by": "crossref",
      "first-page": "25",
      "DOI": "10.1002/j.1460-2075.1985.tb02312.x",
      "volume": "4",
      "author": "PH Schreier",
      "year": "1985",
      "unstructured": "Schreier PH, Seftor EA, Schell J, Bohnert HJ: The use of nuclear encoded sequences to direct the light regulated synthesis and transport of a foreign protein into plant chloroplasts. EMBO J 4: 25?32 (1985).",
      "journal-title": "EMBO J"
    },
    {
      "key": "CR23",
      "doi-asserted-by": "crossref",
      "first-page": "3179",
      "DOI": "10.1093/nar/13.9.3179",
      "volume": "13",
      "author": "S Smeekens",
      "year": "1985",
      "unstructured": "Smeekens S, VanBinsbergen J, Weisbeek P: The plant ferredoxin precursor: nucleotide sequence of a full length cDNA clone. Nucl Acids Res 13: 3179?3194 (1985).",
      "journal-title": "Nucl Acids Res"
    },
    {
      "key": "CR24",
      "doi-asserted-by": "crossref",
      "first-page": "456",
      "DOI": "10.1038/317456a0",
      "volume": "317",
      "author": "S Smeekens",
      "year": "1985",
      "unstructured": "Smeekens S, DeGroot M, VanBinsbergen J, Weisbeek P: Sequence of the precursor of the chloroplast lumen protein plastocyanin. Nature 317: 456?458 (1985).",
      "journal-title": "Nature"
    },
    {
      "key": "CR25",
      "doi-asserted-by": "crossref",
      "first-page": "365",
      "DOI": "10.1016/0092-8674(86)90657-4",
      "volume": "46",
      "author": "S Smeekens",
      "year": "1986",
      "unstructured": "Smeekens S, Bauerle C, Hageman J, Keegstra K, Weisbeek P: The role of the transit peptide in the routing of precursors toward different chloroplast compartments. Cell 46: 365?375 (1986).",
      "journal-title": "Cell"
    },
    {
      "key": "CR26",
      "doi-asserted-by": "crossref",
      "first-page": "3143",
      "DOI": "10.1002/j.1460-2075.1984.tb02271.x",
      "volume": "3",
      "author": "D Steuber",
      "year": "1984",
      "unstructured": "Steuber D, Ibrahimi I, Cutler D, Dobberstein B, Bujard H: A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences. EMBO J 3: 3143?3148 (1984).",
      "journal-title": "EMBO J"
    },
    {
      "key": "CR27",
      "doi-asserted-by": "crossref",
      "first-page": "358",
      "DOI": "10.1038/313358a0",
      "volume": "313",
      "author": "G Broeck Van den",
      "year": "1985",
      "unstructured": "Van denBroeck G, Timko MP, Kausch AP, Cashmore AR, VanMontagu M, Herrera-Estrella L: Targeting of foreign proteins to chloroplasts by fusion to the transit peptide from the small subunit of ribulose-1,5-bisphosphate carboxylase. Nature 313: 358?363 (1985).",
      "journal-title": "Nature"
    },
    {
      "key": "CR28",
      "doi-asserted-by": "crossref",
      "first-page": "801",
      "DOI": "10.1016/0092-8674(86)90846-9",
      "volume": "44",
      "author": "A Loon Van",
      "year": "1986",
      "unstructured": "VanLoon A, Brandli A, Schatz G: The presequences of two imported mitochondrial proteins contain information for intracellular and intramitochondrial sorting. Cell 44: 801?812 (1986).",
      "journal-title": "Cell"
    },
    {
      "key": "CR29",
      "doi-asserted-by": "crossref",
      "first-page": "3643",
      "DOI": "10.1002/j.1460-2075.1986.tb04694.x",
      "volume": "5",
      "author": "H Steeg Van",
      "year": "1986",
      "unstructured": "VanSteeg H, Oudshoorn P, VanHell B, Polman J, Grivell LA: Targeting efficiency of a mitochondrial pre-sequence is dependent on the passenger protein. EMBO J 5: 3643?3650 (1986).",
      "journal-title": "EMBO J"
    },
    {
      "key": "CR30",
      "doi-asserted-by": "crossref",
      "first-page": "446",
      "DOI": "10.1007/BF00338081",
      "volume": "205",
      "author": "C Wasmann",
      "year": "1986",
      "unstructured": "Wasmann C, Reis B, Bartlett S, Bohnert H: The importance of the transit peptide and the transported protein for protein import into chloroplasts. Mol Gen Genet 205: 446?453 (1986).",
      "journal-title": "Mol Gen Genet"
    }
  ],
  "container-title": "Plant Molecular Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://link.springer.com/content/pdf/10.1007/BF00014912.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://link.springer.com/article/10.1007/BF00014912/fulltext.html",
      "content-type": "text/html",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://link.springer.com/content/pdf/10.1007/BF00014912",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        2
      ]
    ],
    "date-time": "2021-07-02T00:13:32Z",
    "timestamp": 1625184812000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://link.springer.com/10.1007/BF00014912"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1987,
        7
      ]
    ]
  },
  "references-count": 30,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1987,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "BF00014912"
  ],
  "URL": "http://dx.doi.org/10.1007/BF00014912",
  "relation": {},
  "ISSN": [
    "0167-4412",
    "1573-5028"
  ],
  "subject": [],
  "container-title-short": "Plant Mol Biol",
  "published": {
    "date-parts": [
      [
        1987,
        7
      ]
    ]
  }
}