DOI: 10.1006/mthe.2000.0257. Cutaneous Transfection and Immune Responses to Intradermal Nucleic Acid Vaccination Are Significantly Enhanced by in Vivo Electropermeabilization

Cutaneous Transfection and Immune Responses to Intradermal Nucleic Acid Vaccination Are Significantly Enhanced by in Vivo Electropermeabilization

10.1006/mthe.2000.0257

Crossref journal-article

Elsevier BV

Molecular Therapy (78)

Bibliography

Drabick, J. J., Glasspool-Malone, J., Somiari, S., King, A., & Malone, R. W. (2001). Cutaneous Transfection and Immune Responses to Intradermal Nucleic Acid Vaccination Are Significantly Enhanced by in Vivo Electropermeabilization. Molecular Therapy, 3(2), 249â255.

Authors 5

Joseph J. Drabick (first)
Jill Glasspool-Malone (additional)
Stella Somiari (additional)
Alan King (additional)
Robert W. Malone (additional)

References 50 Referenced 122

10.1038/sj.gt.3300847 / Gene Ther. / Electropermeabilization of skeletal muscle enhances gene transfer in vivo by Mathiesen (1999)
10.1073/pnas.96.8.4262 / Proc. Natl. Acad. Sci. USA / High-efficiency gene transfer into skeletal muscle mediated by electric pulses by Mir (1999)
10.1089/10430340050015897 / Hum. Gene Ther. / Continuous erythropoietin delivery by muscle-targeted gene transfer using in vivo by Maruyama (2000)
10.4049/jimmunol.164.9.4635 / J. Immunol. / Increased DNA vaccine delivery and immunogenicity by electroporation in vivo by Widera (2000)
10.1016/S0264-410X(00)00087-6 / Vaccine / Protection against influenza virus infection in mice immunized by administration of hemagglutinin-expressing DNAs with electroporation by Kadowaki (2000)
10.1126/science.1690918 / Science / Direct gene transfer into mouse muscle in vivo by Wolff (1990)
10.1073/pnas.91.20.9519 / Proc. Natl. Acad. Sci. USA / Intradermal gene immunization: The possible role of DNA uptake in the induction of cellular immunity to viruses by Raz (1994)
10.1073/pnas.87.24.9568 / Proc. Natl. Acad. Sci. USA / In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment by Yang (1990)
10.1046/j.1525-1381.1999.99223.x / Proc. Assoc. Am. Physicians / A direct in vivo approach for skin gene therapy by Vogel (1999)
10.1016/S0264-410X(96)00265-4 / Vaccine / Biological features of genetic immunization by Barry (1997)
10.1016/S0264-410X(99)00094-8 / Vaccine / Phase 1 safety and immune response studies of a DNA vaccine encoding hepatitis B surface antigen delivered by a gene delivery device by Tacket (1999)
10.1038/nm0597-526 / Nat. Med. / Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination by Boyer (1997)
10.1016/S1357-4310(99)01622-6 / Mol. Med. Today / RNA viruses: Emerging vectors for vaccination and gene therapy by Hewson (2000)
10.1099/0022-1317-80-10-2621 / J. Gen. Virol. / Replication-deficient recombinant adenoviruses expressing the human immunodeficiency virus by Bruce (1999)
10.1006/cimm.1999.1585 / Cell. Immunol. / Enhanced immune response to the melanoma antigen gp100 using recombinant adenovirus-transduced dendritic cells by Wan (1999)
10.1093/hmg/2.11.1847 / Hum. Mol. Genet. / DNA-based immunization induces continuous secretion of hepatitis B surface antigen and high levels of circulating antibody by Davis (1993)
10.1111/j.1749-6632.1995.tb44732.x / Ann. N. Y. Acad. Sci. / DNA-mediated immunization to the hepatitis B surface antigen. Activation and entrainment of the immune response by Whalen (1995)
10.1073/pnas.93.22.12496 / Proc. Natl. Acad. Sci. USA / DNA-mediated immunization in a transgenic mouse model of the hepatitis B surface antigen chronic carrier state by Mancini (1996)
10.1046/j.1525-1381.1999.99227.x / Proc. Assoc. Am. Physicians / Targeting the skin for genetic immunization by Falo (1999)
{'key': '10.1006/mthe.2000.0257_bib20', 'first-page': '84', 'article-title': 'DNA vaccines for prophylactic or therapeutic immunization against hepatitis B virus', 'volume': '66', 'author': 'Davis', 'year': '1999', 'journal-title': 'Mt. Sinai. J. Med.'} / Mt. Sinai. J. Med. / DNA vaccines for prophylactic or therapeutic immunization against hepatitis B virus by Davis (1999)
10.1073/pnas.93.16.8578 / Proc. Natl. Acad. Sci. USA / Induction of cytotoxic T lymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells by Doe (1996)
10.1172/JCI118750 / J. Clin. Invest. / Expression of naked DNA in human, pig, and mouse skin by Hengge (1996)
10.1016/S0264-410X(99)00317-5 / Vaccine / Gene gun mediated vaccination is superior to manual delivery for immunisation with DNA vaccines expressing protective antigens from Yersinia pestis or Venezuelan equine encephalitis virus by Bennett (1999)
10.1038/nm1096-1122 / Nat. Med. / DNA-based immunization by in vivo transfection of dendritic cells by Condon (1996)
10.1016/S0302-4598(99)00008-2 / Bioelectrochem. Bioenerg. / Fundamentals of electroporative delivery of drugs and genes by Neumann (1999)
10.1006/bbrc.1999.1014 / Biochem. Biophys. Res. Commun. / Determination of optimal parameters for in vivo gene transfer by electroporation, using a rapid in vivo test for cell permeabilization by Gehl (1999)
10.1002/(SICI)1097-0142(19980701)83:1<148::AID-CNCR20>3.0.CO;2-W / Cancer / Treatment of cutaneous and subcutaneous tumors with electrochemotherapy using intralesional bleomycin by Heller (1998)
10.1016/S0169-409X(98)00068-4 / Adv. Drug Delivery Rev. / In vivo gene delivery by Heller (1999)
{'key': '10.1006/mthe.2000.0257_bib29', 'series-title': 'Current Protocols in Immunology', 'article-title': 'In situ hybridization for the detection of HIV RNA in cells and tissues', 'author': 'Fox', 'year': '1993'} / Current Protocols in Immunology / In situ hybridization for the detection of HIV RNA in cells and tissues by Fox (1993)
{'key': '10.1006/mthe.2000.0257_bib30', 'first-page': '63', 'article-title': 'Mucosal immune responses associated with polynucleotide vaccination', 'volume': '98', 'author': 'Malone', 'year': '1997', 'journal-title': 'Behring Inst. Mitt.'} / Behring Inst. Mitt. / Mucosal immune responses associated with polynucleotide vaccination by Malone (1997)
10.1038/3266 / Nat. Med. / DNA vaccines with single-chain Fv fused to fragment C of tetanus toxin induce protective immunity against lymphoma and myeloma by King (1998)
10.1046/j.1365-2567.1998.00533.x / Immunology / Intranasal administration of human immunodeficiency virus type-1 (HIV-1) DNA vaccine with interleukin-2 expression plasmid enhances cell-mediated immunity against HIV-1 by Xin (1998)
10.1016/0167-4781(91)90162-F / Biochim. Biophys. Acta / In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA by Titomirov (1991)
10.1006/bbrc.1996.0455 / Biochem. Biophys. Res. Commun. / Depth-targeted efficient gene delivery and expression in the skin by pulsed electric fields: An approach to gene therapy of skin aging and other diseases by Zhang (1996)
10.1016/S0378-5173(98)00085-4 / Int. J. Pharmacol. / Mechanisms of a phosphorothioate oligonucleotide delivery by skin electroporation by Regnier (1999)
10.1016/0006-291X(86)91202-7 / Biochem. Biophys. Res. Commun. / DNA-mediated gene transfer into epidermal cells using electroporation by Reiss (1986)
{'key': '10.1006/mthe.2000.0257_bib37', 'series-title': 'Electroporation and Electrofusion in Cell Biology', 'author': 'Neumann', 'year': '1989'} / Electroporation and Electrofusion in Cell Biology by Neumann (1989)
10.1055/s-2008-1041048 / Semin. Neurol. / Acute electrical burns by ten Duis (1995)
10.4049/jimmunol.164.5.2487 / J. Immunol. / Maturation and trafficking of monocyte-derived dendritic cells in monkeys: Implications for dendritic cell-based vaccines by Barratt-Boyes (2000)
10.1073/pnas.96.5.2285 / Proc. Natl. Acad. Sci. USA / Activation of target-tissue immune-recognition molecules by double-stranded polynucleotides by Suzuki (1999)
10.1073/pnas.93.7.2879 / Proc. Natl. Acad. Sci. USA / CpG motifs present in bacteria DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon gamma by Klinman (1996)
10.1038/15200 / Nat. Med. / Natural adjuvants: Endogenous activators of dendritic cells by Gallucci (1999)
10.1046/j.1365-2893.1999.6120136.x / J. Viral Hepatitis / Gene therapy of viral hepatitis and hepatocellular carcinoma by Ruiz (1999)
10.1126/science.273.5273.352 / Science / Immunostimulatory DNA sequences necessary for effective intradermal gene immunization by Sato (1996)
10.1016/S0264-410X(00)00030-X / Vaccine / Efficient vaccination by intradermal or intramuscular inoculation of plasmid DNA expressing hepatitis B surface antigen under desmin promoter/enhancer control by Kwissa (2000)
10.1007/BF03402065 / Mol. Med. / Route and method of delivery of DNA vaccine influence immune responses in mice and non-human primates by McCluskie (1999)
10.1016/S0264-410X(97)00302-2 / Vaccine / Routes of plasmid DNA vaccination that prime murine humoral and cellular immune responses by Bohm (1998)
10.1073/pnas.95.26.15553 / Proc. Natl. Acad. Sci. USA / CpG DNA can induce strong Th1 humoral and cell-mediated immune responses against hepatitis B surface antigen in young mice by Brazolot Millan (1998)
10.1016/S0264-410X(99)00428-4 / Vaccine / Immunization with a DNA expression vector encoding the varicella zoster virus glycoprotein E (gE) gene via intramuscular and subcutaneous routes by Hasan (2000)
{'key': '10.1006/mthe.2000.0257_bib50', 'first-page': '59', 'article-title': 'A carcinoembryonic antigen polynucleotide vaccine has in vivo antitumor activity', 'volume': '2', 'author': 'Conry', 'year': '1995', 'journal-title': 'Cancer Gene Ther.'} / Cancer Gene Ther. / A carcinoembryonic antigen polynucleotide vaccine has in vivo antitumor activity by Conry (1995)

Dates

Type	When
Created	22 years, 11 months ago (Sept. 18, 2002, 3:46 p.m.)
Deposited	4 years, 5 months ago (March 10, 2021, 10:47 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 3, 2024, 4:51 p.m.)
Issued	24 years, 6 months ago (Feb. 1, 2001)
Published	24 years, 6 months ago (Feb. 1, 2001)
Published Print	24 years, 6 months ago (Feb. 1, 2001)

Funders 0

None

BibTeX

@article{Drabick_2001, title={Cutaneous Transfection and Immune Responses to Intradermal Nucleic Acid Vaccination Are Significantly Enhanced by in Vivo Electropermeabilization}, volume={3}, ISSN={1525-0016}, url={http://dx.doi.org/10.1006/mthe.2000.0257}, DOI={10.1006/mthe.2000.0257}, number={2}, journal={Molecular Therapy}, publisher={Elsevier BV}, author={Drabick, Joseph J. and Glasspool-Malone, Jill and Somiari, Stella and King, Alan and Malone, Robert W.}, year={2001}, month=feb, pages={249–255} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        3
      ]
    ],
    "date-time": "2024-09-03T20:51:08Z",
    "timestamp": 1725396668175
  },
  "reference-count": 50,
  "publisher": "Elsevier BV",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2001,
            2,
            1
          ]
        ],
        "date-time": "2001-02-01T00:00:00Z",
        "timestamp": 980985600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://www.elsevier.com/tdm/userlicense/1.0/"
    },
    {
      "start": {
        "date-parts": [
          [
            2016,
            10,
            7
          ]
        ],
        "date-time": "2016-10-07T00:00:00Z",
        "timestamp": 1475798400000
      },
      "content-version": "vor",
      "delay-in-days": 5727,
      "URL": "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        2
      ]
    ]
  },
  "DOI": "10.1006/mthe.2000.0257",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        9,
        18
      ]
    ],
    "date-time": "2002-09-18T19:46:43Z",
    "timestamp": 1032378403000
  },
  "page": "249-255",
  "source": "Crossref",
  "is-referenced-by-count": 122,
  "title": "Cutaneous Transfection and Immune Responses to Intradermal Nucleic Acid Vaccination Are Significantly Enhanced by in Vivo Electropermeabilization",
  "prefix": "10.1016",
  "volume": "3",
  "author": [
    {
      "given": "Joseph J.",
      "family": "Drabick",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Jill",
      "family": "Glasspool-Malone",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Stella",
      "family": "Somiari",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Alan",
      "family": "King",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Robert W.",
      "family": "Malone",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "78",
  "reference": [
    {
      "key": "10.1006/mthe.2000.0257_bib1",
      "doi-asserted-by": "crossref",
      "first-page": "508",
      "DOI": "10.1038/sj.gt.3300847",
      "article-title": "Electropermeabilization of skeletal muscle enhances gene transfer in vivo",
      "volume": "6",
      "author": "Mathiesen",
      "year": "1999",
      "journal-title": "Gene Ther."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib2",
      "doi-asserted-by": "crossref",
      "first-page": "4262",
      "DOI": "10.1073/pnas.96.8.4262",
      "article-title": "High-efficiency gene transfer into skeletal muscle mediated by electric pulses",
      "volume": "96",
      "author": "Mir",
      "year": "1999",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib3",
      "doi-asserted-by": "crossref",
      "first-page": "429",
      "DOI": "10.1089/10430340050015897",
      "article-title": "Continuous erythropoietin delivery by muscle-targeted gene transfer using in vivo",
      "volume": "11",
      "author": "Maruyama",
      "year": "2000",
      "journal-title": "Hum. Gene Ther."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib4",
      "doi-asserted-by": "crossref",
      "first-page": "4635",
      "DOI": "10.4049/jimmunol.164.9.4635",
      "article-title": "Increased DNA vaccine delivery and immunogenicity by electroporation in vivo",
      "volume": "164",
      "author": "Widera",
      "year": "2000",
      "journal-title": "J. Immunol."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib5",
      "doi-asserted-by": "crossref",
      "first-page": "2779",
      "DOI": "10.1016/S0264-410X(00)00087-6",
      "article-title": "Protection against influenza virus infection in mice immunized by administration of hemagglutinin-expressing DNAs with electroporation",
      "volume": "18",
      "author": "Kadowaki",
      "year": "2000",
      "journal-title": "Vaccine"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib6",
      "doi-asserted-by": "crossref",
      "first-page": "1465",
      "DOI": "10.1126/science.1690918",
      "article-title": "Direct gene transfer into mouse muscle in vivo",
      "volume": "247",
      "author": "Wolff",
      "year": "1990",
      "journal-title": "Science"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib7",
      "doi-asserted-by": "crossref",
      "first-page": "9519",
      "DOI": "10.1073/pnas.91.20.9519",
      "article-title": "Intradermal gene immunization: The possible role of DNA uptake in the induction of cellular immunity to viruses",
      "volume": "91",
      "author": "Raz",
      "year": "1994",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib8",
      "doi-asserted-by": "crossref",
      "first-page": "9568",
      "DOI": "10.1073/pnas.87.24.9568",
      "article-title": "In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment",
      "volume": "87",
      "author": "Yang",
      "year": "1990",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib9",
      "doi-asserted-by": "crossref",
      "first-page": "190",
      "DOI": "10.1046/j.1525-1381.1999.99223.x",
      "article-title": "A direct in vivo approach for skin gene therapy",
      "volume": "111",
      "author": "Vogel",
      "year": "1999",
      "journal-title": "Proc. Assoc. Am. Physicians"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib10",
      "doi-asserted-by": "crossref",
      "first-page": "788",
      "DOI": "10.1016/S0264-410X(96)00265-4",
      "article-title": "Biological features of genetic immunization",
      "volume": "15",
      "author": "Barry",
      "year": "1997",
      "journal-title": "Vaccine"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib11",
      "doi-asserted-by": "crossref",
      "first-page": "2826",
      "DOI": "10.1016/S0264-410X(99)00094-8",
      "article-title": "Phase 1 safety and immune response studies of a DNA vaccine encoding hepatitis B surface antigen delivered by a gene delivery device",
      "volume": "17",
      "author": "Tacket",
      "year": "1999",
      "journal-title": "Vaccine"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib12",
      "doi-asserted-by": "crossref",
      "first-page": "526",
      "DOI": "10.1038/nm0597-526",
      "article-title": "Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination",
      "volume": "3",
      "author": "Boyer",
      "year": "1997",
      "journal-title": "Nat. Med."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib13",
      "doi-asserted-by": "crossref",
      "first-page": "28",
      "DOI": "10.1016/S1357-4310(99)01622-6",
      "article-title": "RNA viruses: Emerging vectors for vaccination and gene therapy",
      "volume": "6",
      "author": "Hewson",
      "year": "2000",
      "journal-title": "Mol. Med. Today"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib14",
      "doi-asserted-by": "crossref",
      "first-page": "2621",
      "DOI": "10.1099/0022-1317-80-10-2621",
      "article-title": "Replication-deficient recombinant adenoviruses expressing the human immunodeficiency virus",
      "volume": "80",
      "author": "Bruce",
      "year": "1999",
      "journal-title": "J. Gen. Virol."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib15",
      "doi-asserted-by": "crossref",
      "first-page": "131",
      "DOI": "10.1006/cimm.1999.1585",
      "article-title": "Enhanced immune response to the melanoma antigen gp100 using recombinant adenovirus-transduced dendritic cells",
      "volume": "198",
      "author": "Wan",
      "year": "1999",
      "journal-title": "Cell. Immunol."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib16",
      "doi-asserted-by": "crossref",
      "first-page": "1847",
      "DOI": "10.1093/hmg/2.11.1847",
      "article-title": "DNA-based immunization induces continuous secretion of hepatitis B surface antigen and high levels of circulating antibody",
      "volume": "2",
      "author": "Davis",
      "year": "1993",
      "journal-title": "Hum. Mol. Genet."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib17",
      "doi-asserted-by": "crossref",
      "first-page": "64",
      "DOI": "10.1111/j.1749-6632.1995.tb44732.x",
      "article-title": "DNA-mediated immunization to the hepatitis B surface antigen. Activation and entrainment of the immune response",
      "volume": "772",
      "author": "Whalen",
      "year": "1995",
      "journal-title": "Ann. N. Y. Acad. Sci."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib18",
      "doi-asserted-by": "crossref",
      "first-page": "12496",
      "DOI": "10.1073/pnas.93.22.12496",
      "article-title": "DNA-mediated immunization in a transgenic mouse model of the hepatitis B surface antigen chronic carrier state",
      "volume": "93",
      "author": "Mancini",
      "year": "1996",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib19",
      "doi-asserted-by": "crossref",
      "first-page": "211",
      "DOI": "10.1046/j.1525-1381.1999.99227.x",
      "article-title": "Targeting the skin for genetic immunization",
      "volume": "111",
      "author": "Falo",
      "year": "1999",
      "journal-title": "Proc. Assoc. Am. Physicians"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib20",
      "first-page": "84",
      "article-title": "DNA vaccines for prophylactic or therapeutic immunization against hepatitis B virus",
      "volume": "66",
      "author": "Davis",
      "year": "1999",
      "journal-title": "Mt. Sinai. J. Med."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib21",
      "doi-asserted-by": "crossref",
      "first-page": "8578",
      "DOI": "10.1073/pnas.93.16.8578",
      "article-title": "Induction of cytotoxic T lymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells",
      "volume": "93",
      "author": "Doe",
      "year": "1996",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib22",
      "doi-asserted-by": "crossref",
      "first-page": "2911",
      "DOI": "10.1172/JCI118750",
      "article-title": "Expression of naked DNA in human, pig, and mouse skin",
      "volume": "97",
      "author": "Hengge",
      "year": "1996",
      "journal-title": "J. Clin. Invest."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib23",
      "doi-asserted-by": "crossref",
      "first-page": "588",
      "DOI": "10.1016/S0264-410X(99)00317-5",
      "article-title": "Gene gun mediated vaccination is superior to manual delivery for immunisation with DNA vaccines expressing protective antigens from Yersinia pestis or Venezuelan equine encephalitis virus",
      "volume": "18",
      "author": "Bennett",
      "year": "1999",
      "journal-title": "Vaccine"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib24",
      "doi-asserted-by": "crossref",
      "first-page": "1122",
      "DOI": "10.1038/nm1096-1122",
      "article-title": "DNA-based immunization by in vivo transfection of dendritic cells",
      "volume": "2",
      "author": "Condon",
      "year": "1996",
      "journal-title": "Nat. Med."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib25",
      "doi-asserted-by": "crossref",
      "first-page": "3",
      "DOI": "10.1016/S0302-4598(99)00008-2",
      "article-title": "Fundamentals of electroporative delivery of drugs and genes",
      "volume": "48",
      "author": "Neumann",
      "year": "1999",
      "journal-title": "Bioelectrochem. Bioenerg."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib26",
      "doi-asserted-by": "crossref",
      "first-page": "377",
      "DOI": "10.1006/bbrc.1999.1014",
      "article-title": "Determination of optimal parameters for in vivo gene transfer by electroporation, using a rapid in vivo test for cell permeabilization",
      "volume": "261",
      "author": "Gehl",
      "year": "1999",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib27",
      "doi-asserted-by": "crossref",
      "first-page": "148",
      "DOI": "10.1002/(SICI)1097-0142(19980701)83:1<148::AID-CNCR20>3.0.CO;2-W",
      "article-title": "Treatment of cutaneous and subcutaneous tumors with electrochemotherapy using intralesional bleomycin",
      "volume": "83",
      "author": "Heller",
      "year": "1998",
      "journal-title": "Cancer"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib28",
      "doi-asserted-by": "crossref",
      "first-page": "131",
      "DOI": "10.1016/S0169-409X(98)00068-4",
      "article-title": "In vivo gene delivery",
      "volume": "35",
      "author": "Heller",
      "year": "1999",
      "journal-title": "Adv. Drug Delivery Rev."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib29",
      "series-title": "Current Protocols in Immunology",
      "article-title": "In situ hybridization for the detection of HIV RNA in cells and tissues",
      "author": "Fox",
      "year": "1993"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib30",
      "first-page": "63",
      "article-title": "Mucosal immune responses associated with polynucleotide vaccination",
      "volume": "98",
      "author": "Malone",
      "year": "1997",
      "journal-title": "Behring Inst. Mitt."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib31",
      "doi-asserted-by": "crossref",
      "first-page": "1281",
      "DOI": "10.1038/3266",
      "article-title": "DNA vaccines with single-chain Fv fused to fragment C of tetanus toxin induce protective immunity against lymphoma and myeloma",
      "volume": "4",
      "author": "King",
      "year": "1998",
      "journal-title": "Nat. Med."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib32",
      "doi-asserted-by": "crossref",
      "first-page": "438",
      "DOI": "10.1046/j.1365-2567.1998.00533.x",
      "article-title": "Intranasal administration of human immunodeficiency virus type-1 (HIV-1) DNA vaccine with interleukin-2 expression plasmid enhances cell-mediated immunity against HIV-1",
      "volume": "94",
      "author": "Xin",
      "year": "1998",
      "journal-title": "Immunology"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib33",
      "doi-asserted-by": "crossref",
      "first-page": "131",
      "DOI": "10.1016/0167-4781(91)90162-F",
      "article-title": "In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA",
      "volume": "1088",
      "author": "Titomirov",
      "year": "1991",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib34",
      "doi-asserted-by": "crossref",
      "first-page": "633",
      "DOI": "10.1006/bbrc.1996.0455",
      "article-title": "Depth-targeted efficient gene delivery and expression in the skin by pulsed electric fields: An approach to gene therapy of skin aging and other diseases",
      "volume": "220",
      "author": "Zhang",
      "year": "1996",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib35",
      "doi-asserted-by": "crossref",
      "first-page": "147",
      "DOI": "10.1016/S0378-5173(98)00085-4",
      "article-title": "Mechanisms of a phosphorothioate oligonucleotide delivery by skin electroporation",
      "volume": "184",
      "author": "Regnier",
      "year": "1999",
      "journal-title": "Int. J. Pharmacol."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib36",
      "doi-asserted-by": "crossref",
      "first-page": "244",
      "DOI": "10.1016/0006-291X(86)91202-7",
      "article-title": "DNA-mediated gene transfer into epidermal cells using electroporation",
      "volume": "137",
      "author": "Reiss",
      "year": "1986",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib37",
      "series-title": "Electroporation and Electrofusion in Cell Biology",
      "author": "Neumann",
      "year": "1989"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib38",
      "doi-asserted-by": "crossref",
      "first-page": "381",
      "DOI": "10.1055/s-2008-1041048",
      "article-title": "Acute electrical burns",
      "volume": "15",
      "author": "ten Duis",
      "year": "1995",
      "journal-title": "Semin. Neurol."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib39",
      "doi-asserted-by": "crossref",
      "first-page": "2487",
      "DOI": "10.4049/jimmunol.164.5.2487",
      "article-title": "Maturation and trafficking of monocyte-derived dendritic cells in monkeys: Implications for dendritic cell-based vaccines",
      "volume": "164",
      "author": "Barratt-Boyes",
      "year": "2000",
      "journal-title": "J. Immunol."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib40",
      "doi-asserted-by": "crossref",
      "first-page": "2285",
      "DOI": "10.1073/pnas.96.5.2285",
      "article-title": "Activation of target-tissue immune-recognition molecules by double-stranded polynucleotides",
      "volume": "96",
      "author": "Suzuki",
      "year": "1999",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib41",
      "doi-asserted-by": "crossref",
      "first-page": "2879",
      "DOI": "10.1073/pnas.93.7.2879",
      "article-title": "CpG motifs present in bacteria DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon gamma",
      "volume": "93",
      "author": "Klinman",
      "year": "1996",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib42",
      "doi-asserted-by": "crossref",
      "first-page": "1249",
      "DOI": "10.1038/15200",
      "article-title": "Natural adjuvants: Endogenous activators of dendritic cells",
      "volume": "5",
      "author": "Gallucci",
      "year": "1999",
      "journal-title": "Nat. Med."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib43",
      "doi-asserted-by": "crossref",
      "first-page": "17",
      "DOI": "10.1046/j.1365-2893.1999.6120136.x",
      "article-title": "Gene therapy of viral hepatitis and hepatocellular carcinoma",
      "volume": "6",
      "author": "Ruiz",
      "year": "1999",
      "journal-title": "J. Viral Hepatitis"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib44",
      "doi-asserted-by": "crossref",
      "first-page": "352",
      "DOI": "10.1126/science.273.5273.352",
      "article-title": "Immunostimulatory DNA sequences necessary for effective intradermal gene immunization",
      "volume": "273",
      "author": "Sato",
      "year": "1996",
      "journal-title": "Science"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib45",
      "doi-asserted-by": "crossref",
      "first-page": "2337",
      "DOI": "10.1016/S0264-410X(00)00030-X",
      "article-title": "Efficient vaccination by intradermal or intramuscular inoculation of plasmid DNA expressing hepatitis B surface antigen under desmin promoter/enhancer control",
      "volume": "18",
      "author": "Kwissa",
      "year": "2000",
      "journal-title": "Vaccine"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib46",
      "doi-asserted-by": "crossref",
      "first-page": "287",
      "DOI": "10.1007/BF03402065",
      "article-title": "Route and method of delivery of DNA vaccine influence immune responses in mice and non-human primates",
      "volume": "5",
      "author": "McCluskie",
      "year": "1999",
      "journal-title": "Mol. Med."
    },
    {
      "key": "10.1006/mthe.2000.0257_bib47",
      "doi-asserted-by": "crossref",
      "first-page": "949",
      "DOI": "10.1016/S0264-410X(97)00302-2",
      "article-title": "Routes of plasmid DNA vaccination that prime murine humoral and cellular immune responses",
      "volume": "16",
      "author": "Bohm",
      "year": "1998",
      "journal-title": "Vaccine"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib48",
      "doi-asserted-by": "crossref",
      "first-page": "15553",
      "DOI": "10.1073/pnas.95.26.15553",
      "article-title": "CpG DNA can induce strong Th1 humoral and cell-mediated immune responses against hepatitis B surface antigen in young mice",
      "volume": "95",
      "author": "Brazolot Millan",
      "year": "1998",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib49",
      "doi-asserted-by": "crossref",
      "first-page": "1506",
      "DOI": "10.1016/S0264-410X(99)00428-4",
      "article-title": "Immunization with a DNA expression vector encoding the varicella zoster virus glycoprotein E (gE) gene via intramuscular and subcutaneous routes",
      "volume": "14",
      "author": "Hasan",
      "year": "2000",
      "journal-title": "Vaccine"
    },
    {
      "key": "10.1006/mthe.2000.0257_bib50",
      "first-page": "59",
      "article-title": "A carcinoembryonic antigen polynucleotide vaccine has in vivo antitumor activity",
      "volume": "2",
      "author": "Conry",
      "year": "1995",
      "journal-title": "Cancer Gene Ther."
    }
  ],
  "container-title": "Molecular Therapy",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.elsevier.com/content/article/PII:S1525001600902570?httpAccept=text/xml",
      "content-type": "text/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.elsevier.com/content/article/PII:S1525001600902570?httpAccept=text/plain",
      "content-type": "text/plain",
      "content-version": "vor",
      "intended-application": "text-mining"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        3,
        11
      ]
    ],
    "date-time": "2021-03-11T03:47:47Z",
    "timestamp": 1615434467000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://linkinghub.elsevier.com/retrieve/pii/S1525001600902570"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        2
      ]
    ]
  },
  "references-count": 50,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          2001,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "S1525001600902570"
  ],
  "URL": "http://dx.doi.org/10.1006/mthe.2000.0257",
  "relation": {},
  "ISSN": [
    "1525-0016"
  ],
  "subject": [],
  "container-title-short": "Molecular Therapy",
  "published": {
    "date-parts": [
      [
        2001,
        2
      ]
    ]
  }
}