DOI: 10.1006/cbir.2002.0917. FRACTIONATION ANALYSIS OF THE ENDOSOMAL COMPARTMENT DURING RAT RETICULOCYTE MATURATION

FRACTIONATION ANALYSIS OF THE ENDOSOMAL COMPARTMENT DURING RAT RETICULOCYTE MATURATION

10.1006/cbir.2002.0917

Crossref journal-article

Wiley

Cell Biology International (311)

Abstract

AbstractA subcellular fractionation procedure was developed to isolate the different endosomal compartments present during reticulocyte maturation. After reticulocyte lysis and removal of excess haemoglobin by gel chromatography, membrane vesicles were separated over a discontinuous sucrose gradient (10–40%). Two fractions were isolated: P1 at the 25–35% sucrose interface and P2 at the 17–25% sucrose interface. These fractions were morphologically characterized by electron microscopy and the distribution of endocytic markers in the fractions was detected by Western blot. Moreover, this fractionation technique was used to study the effect of 3‐methyladenine (3‐MA), an autophagy inhibitor, on reticulocyte maturation. The presence of 3‐MA during in vitro maturation of reticulocytes induced a decrease in exosome secretion, as measured by the amount of transferrin receptor (TfR) released in the extracellular medium. The subcellular fractionation results suggested that multivesicular endosome formation from early endosomes is the step affected by 3‐MA.

Bibliography

Dardalhon, V., GÃ©minard, C., Reggio, H., Vidal, M., & SainteâMarie, J. (2002). FRACTIONATION ANALYSIS OF THE ENDOSOMAL COMPARTMENT DURING RAT RETICULOCYTE MATURATION. Cell Biology International, 26(8), 669â678. Portico.

Authors 5

Valérie Dardalhon (first)
Charles Géminard (additional)
Hubert Reggio (additional)
Michel Vidal (additional)
Josette Sainte‐Marie (additional)

References 0 Referenced 15

None

Dates

Type	When
Created	23 years ago (Aug. 28, 2002, 12:33 p.m.)
Deposited	1 year, 9 months ago (Nov. 18, 2023, 5:31 a.m.)
Indexed	1 year, 1 month ago (July 27, 2024, 5:13 a.m.)
Issued	23 years ago (Aug. 1, 2002)
Published	23 years ago (Aug. 1, 2002)
Published Online	12 years, 7 months ago (Jan. 2, 2013)
Published Print	23 years ago (Aug. 1, 2002)

Funders 0

None

BibTeX

@article{Dardalhon_2002, title={FRACTIONATION ANALYSIS OF THE ENDOSOMAL COMPARTMENT DURING RAT RETICULOCYTE MATURATION}, volume={26}, ISSN={1095-8355}, url={http://dx.doi.org/10.1006/cbir.2002.0917}, DOI={10.1006/cbir.2002.0917}, number={8}, journal={Cell Biology International}, publisher={Wiley}, author={Dardalhon, Valérie and Géminard, Charles and Reggio, Hubert and Vidal, Michel and Sainte‐Marie, Josette}, year={2002}, month=aug, pages={669–678} }

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