DOI: 10.1006/cbir.1993.1079. Study of localization of the protein‐synthesizing machinery along actin filament bundles.

Study of localization of the protein‐synthesizing machinery along actin filament bundles.

10.1006/cbir.1993.1079

Crossref journal-article

Wiley

Cell Biology International (311)

Abstract

AbstractIndirect immunofluorescent microscopy was used to study the distribution of elongation factor 2 (eEF‐2) in fixed human skin diploid and mouse embryo fibroblasts. It was found earlier that some of the eEF‐2 ribosomes and initiation factor 2 (eIF‐2) are co‐localized with a part of the actin microfilament bundles in these cells (Gavrilova et al., 1987; Shestakova et al., 1991). Here it has been shown that inhibition of protein synthesis either by inactivation of eEF‐2 itself with diphtheria toxin or by inactivation of ribosomes with ricin does not abolish the distribution of eEF‐2 along the actin microfilament bundles. At the same time, the disassembly of actin microfilaments by cytochalasin D results also in the disappearance of eEF‐2‐carrying threads. This means that the eEF‐2‐carrying threads do not exist per se, and that the organization of eEF‐2 in visible "filaments" depends upon the integrity of the actin cytoskeleton.

Bibliography

Shestakova, E. A., Motuz, L. P., Minin, A. A., & Gavrilova, L. P. (1993). Study of localization of the proteinâsynthesizing machinery along actin filament bundles. Cell Biology International, 17(4), 409â416. Portico.

Authors 4

Elena A. Shestakova (first)
Ludmila P. Motuz (additional)
Alexander A. Minin (additional)
Lydia P. Gavrilova (additional)

References 0 Referenced 22

None

Dates

Type	When
Created	22 years, 10 months ago (Oct. 6, 2002, 6:25 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 12:56 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:29 a.m.)
Issued	32 years, 5 months ago (April 1, 1993)
Published	32 years, 5 months ago (April 1, 1993)
Published Online	12 years, 8 months ago (Jan. 2, 2013)
Published Print	32 years, 5 months ago (April 1, 1993)

Funders 0

None

BibTeX

@article{Shestakova_1993, title={Study of localization of the protein‐synthesizing machinery along actin filament bundles.}, volume={17}, ISSN={1095-8355}, url={http://dx.doi.org/10.1006/cbir.1993.1079}, DOI={10.1006/cbir.1993.1079}, number={4}, journal={Cell Biology International}, publisher={Wiley}, author={Shestakova, Elena A. and Motuz, Ludmila P. and Minin, Alexander A. and Gavrilova, Lydia P.}, year={1993}, month=apr, pages={409–416} }

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