DOI: 10.1002/yea.838. Mutants defective in secretory/vacuolar pathways in the EUROFAN collection of yeast disruptants

Mutants defective in secretory/vacuolar pathways in the EUROFAN collection of yeast disruptants

10.1002/yea.838

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractWe have screened the EUROFAN (European Functional Analysis Network) deletion strain collection for yeast mutants defective in secretory/vacuolar pathways and/or associated biochemical modifications. We used systematic Western immunoblotting to analyse the electrophoretic pattern of several markers of the secretory/vacuolar pathways, the soluble α‐factor, the periplasmic glycoprotein invertase, the plasma membrane GPI‐anchored protein Gas1p, and two vacuolar proteins, the soluble carboxypeptidase Y and the membrane‐bound alkaline phosphatase, which are targeted to the vacuole by different pathways. We also used colony immunoblotting to monitor the secretion of carboxypeptidase Y into the medium, to identify disruptants impaired in vacuolar targeting. We identified 25 mutants among the 631 deletion strains. Nine of these mutants were disrupted in genes identified in recent years on the basis of their involvement in trafficking (VPS53, VAC7, VAM6, APM3, SYS1), or glycosylation (ALG12, ALG9, OST4, ROT2). Three of these genes were identified on the basis of trafficking defects by ourselves and others within the EUROFAN project (TLG2, RCY1, MON2). The deletion of ERV29, which encodes a COPII vesicle protein, impaired carboxypeptidase Y trafficking from the endoplasmic reticulum to the Golgi apparatus. We also identified eight unknown ORFs, the deletion of which reduced Golgi glycosylation or impaired the Golgi to vacuole trafficking of carboxypeptidase Y. YJR044c, which we identified as a new VPS gene, encodes a protein with numerous homologues of unknown function in sequence databases. Copyright © 2002 John Wiley & Sons, Ltd.

Bibliography

Avaro, S., BelgarehâTouzÃ©, N., SibellaâArgÃ¼elles, C., Volland, C., & HaguenauerâTsapis, R. (2002). Mutants defective in secretory/vacuolar pathways in the EUROFAN collection of yeast disruptants. Yeast, 19(4), 351â371. Portico.

Authors 5

Sandrine Avaro (first)
Naïma Belgareh‐Touzé (additional)
Carla Sibella‐Argüelles (additional)
Christiane Volland (additional)
Rosine Haguenauer‐Tsapis (additional)

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Dates

Type	When
Created	23 years ago (Aug. 25, 2002, 7:07 p.m.)
Deposited	1 year, 9 months ago (Nov. 18, 2023, 10:46 a.m.)
Indexed	6 months, 1 week ago (Feb. 20, 2025, 9:17 p.m.)
Issued	23 years, 6 months ago (Feb. 21, 2002)
Published	23 years, 6 months ago (Feb. 21, 2002)
Published Online	23 years, 6 months ago (Feb. 21, 2002)
Published Print	23 years, 5 months ago (March 15, 2002)

Funders 2

Centre National de la Recherche Scientifique, France 10.13039/501100004794 Centre National de la Recherche Scientifique
Region: Europe
gov (Research institutes and centers)
Labels4
1. National Centre for Scientific Research
2. French National Centre for Scientific Research
3. Le Centre national de la recherche scientifique
4. CNRS
EUROFAN project of the Eu
Awards1
1. BIO4CT 97-2294

BibTeX

@article{Avaro_2002, title={Mutants defective in secretory/vacuolar pathways in the EUROFAN collection of yeast disruptants}, volume={19}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.838}, DOI={10.1002/yea.838}, number={4}, journal={Yeast}, publisher={Wiley}, author={Avaro, Sandrine and Belgareh‐Touzé, Naïma and Sibella‐Argüelles, Carla and Volland, Christiane and Haguenauer‐Tsapis, Rosine}, year={2002}, month=feb, pages={351–371} }

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