DOI: 10.1002/yea.726. Identification and characterization of <i>Saccharomyces cerevisiae</i> mutants defective in fluid‐phase endocytosis

Identification and characterization of Saccharomyces cerevisiae mutants defective in fluid‐phase endocytosis

10.1002/yea.726

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractA mutant library generated by the European Functional Analysis Network (EUROFAN) was screened for strains defective in fluid‐phase endocytosis. Accumulation of Lucifer yellow in the vacuole was used as a marker for efficient endocytosis. Fourteen mutants, including ede1Δ, rcy1Δ, sys1Δ and tlg2Δ, previously described to be involved in membrane trafficking, were identified in this screen. α‐Factor uptake, endocytosis of FM4‐64, carboxypeptidase Y secretion, vacuolar morphology, and a vma2 synthetic growth defect were used as criteria to characterize the endocytic defect of the mutant strains obtained. Accordingly, eight mutant strains have endocytic phenotypes in addition to their defect in Lucifer yellow accumulation. These fluid‐phase endocytosis mutants are defective at different steps of the endocytic pathway. Interestingly, only two mutants were defective for internalization, two for vacuolar protein sorting and four mutants had aberrant vacuolar morphologies. Some of the mutants identified in this screen that sort carboxypeptidase Y correctly may affect endocytosis at an early post‐internalization step before the intersection of the endocytic with the vacuolar protein‐sorting pathway. Copyright © 2001 John Wiley & Sons, Ltd.

Bibliography

Wiederkehr, A., Meier, K. D., & Riezman, H. (2001). Identification and characterization of Saccharomyces cerevisiae mutants defective in fluidâphase endocytosis. Yeast, 18(8), 759â773. Portico.

Authors 3

Andreas Wiederkehr (first)
Karsten D. Meier (additional)
Howard Riezman (additional)

References 48 Referenced 58

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{'key': 'e_1_2_7_22_1', 'first-page': '(in press)', 'article-title': 'Identification of yeast deletion strains that are hypersensitive to brefeldin A or monensin, two drugs that affect intracellular transport', 'author': 'Muren E', 'year': '2000', 'journal-title': 'Yeast'} / Yeast / Identification of yeast deletion strains that are hypersensitive to brefeldin A or monensin, two drugs that affect intracellular transport by Muren E (2000)
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Dates

Type	When
Created	23 years ago (Aug. 25, 2002, 6:10 p.m.)
Deposited	1 year, 9 months ago (Nov. 18, 2023, 6:22 a.m.)
Indexed	1 year ago (Aug. 20, 2024, 2:32 a.m.)
Issued	24 years, 5 months ago (March 30, 2001)
Published	24 years, 5 months ago (March 30, 2001)
Published Online	24 years, 5 months ago (March 30, 2001)
Published Print	24 years, 2 months ago (June 15, 2001)

Funders 0

None

BibTeX

@article{Wiederkehr_2001, title={Identification and characterization of Saccharomyces cerevisiae mutants defective in fluid‐phase endocytosis}, volume={18}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.726}, DOI={10.1002/yea.726}, number={8}, journal={Yeast}, publisher={Wiley}, author={Wiederkehr, Andreas and Meier, Karsten D. and Riezman, Howard}, year={2001}, month=mar, pages={759–773} }

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