DOI: 10.1002/yea.320110702. Two‐Dimensional protein map of <i>Saccharomyces cerevisiae</i>: Construction of a gene

Two‐Dimensional protein map of Saccharomyces cerevisiae: Construction of a gene–protein index

10.1002/yea.320110702

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractThis publication marks the beginning of the construction of a gene–protein index that relates proteins which are resolved on the two‐dimensional protein map of Saccharomyces cerevisiae with their corresponding genes. We report the identification of 36 novel polypeptide spots on the yeast protein map. They correspond to the products of 26 genes. Together with the polypeptide spots previously identified, this raises to 41 the number of genes whose products have been identified on the protein map. The proteins identified here are concerned with four major areas of yeast cellular physiology: carbon metabolism, heat shock, amino acid biosynthesis and purine biosynthesis. Given the molecular weight and isoelectric point of the identified proteins, and the codon‐usage bias of the corresponding genes, it can be estimated that 25 to 35% of all the soluble yeast proteins are detectable under the labelling and running gel conditions used in this study.

Bibliography

Boucherie, H., Monribot, C., Perrot, M., Dujardin, G., Kermorgant, M., & Slonimski, P. (1995). TwoâDimensional protein map of Saccharomyces cerevisiae: Construction of a geneâprotein index. Yeast, 11(7), 601â613. Portico.

Authors 6

Helian Boucherie (first)
Christelle Monribot (additional)
Michel Perrot (additional)
Genevieve Dujardin (additional)
Michele Kermorgant (additional)
Piotr Slonimski (additional)

References 33 Referenced 73

10.1007/BF00330521
10.1002/yea.320030104
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10.1093/genetics/113.1.35 / Genetics / Genealogy of principle strains of the yeast genetic stock center by Mortimer R. K. (1986)
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{'key': 'e_1_2_1_18_1', 'first-page': '710', 'volume-title': 'Methods in Enzymology', 'author': 'Nicolet C. M.', 'year': '1991'} / Methods in Enzymology by Nicolet C. M. (1991)
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{'key': 'e_1_2_1_32_1', 'first-page': '1037', 'article-title': 'Host‐plasmid interactions in Saccharomyces cerevisiae: effect of host ploidy on plasmid stability and copy number', 'volume': '135', 'author': 'Spalding A.', 'year': '1989', 'journal-title': 'J. Gen. Microbiol.'} / J. Gen. Microbiol. / Host‐plasmid interactions in Saccharomyces cerevisiae: effect of host ploidy on plasmid stability and copy number by Spalding A. (1989)
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Dates

Type	When
Created	20 years, 2 months ago (May 28, 2005, 7:59 p.m.)
Deposited	1 year, 9 months ago (Oct. 26, 2023, 7:20 p.m.)
Indexed	2 months, 3 weeks ago (May 26, 2025, 7:23 a.m.)
Issued	30 years, 2 months ago (June 15, 1995)
Published	30 years, 2 months ago (June 15, 1995)
Published Online	21 years, 6 months ago (Jan. 29, 2004)
Published Print	30 years, 2 months ago (June 15, 1995)

Funders 0

None

BibTeX

@article{Boucherie_1995, title={Two‐Dimensional protein map of Saccharomyces cerevisiae: Construction of a gene–protein index}, volume={11}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.320110702}, DOI={10.1002/yea.320110702}, number={7}, journal={Yeast}, publisher={Wiley}, author={Boucherie, Helian and Monribot, Christelle and Perrot, Michel and Dujardin, Genevieve and Kermorgant, Michele and Slonimski, Piotr}, year={1995}, month=jun, pages={601–613} }

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