DOI: 10.1002/yea.320110105. A deviation from the universal genetic code in <i>Candida maltosa</i> and consequences for heterologous expression of cytochromes P450 52A4 and 52A5 in <i>Saccharomyces cerevisiae</i>

A deviation from the universal genetic code in Candida maltosa and consequences for heterologous expression of cytochromes P450 52A4 and 52A5 in Saccharomyces cerevisiae

10.1002/yea.320110105

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractWe demonstrate that serine instead of leucine is specified by the CUG codon in the yeast Candida maltosa. Evidence for this deviation from the universal genetic code was obtained by means of in vitro translation experiments. Depending on the cell‐free system used, either serine, in the C. maltosa system, or leucine, in the control with the conventional wheat germ system, was found to be incorporated into the translation products of artificial CUG‐containing mRNAs. Moreover, we were able to transfer the non‐universal decoding of CUG to the wheat germ system by adding a tRNA fraction isolated from C. maltosa. This finding indicates the presence in C. maltosa of an unusual serine tRNA that recognizes CUG. As a consequence of the altered genetic code, expression in Saccharomyces cerevisiae of C. maltosa cytochrome P450 genes required an exchange of their CTG triplets by TCT encoding serine in order to produce the authentic proteins. In contrast, heterologous expression of the original C. maltosa genes resulted in the formation of still active but unstable enzymes probably subject to selective proteolysis in the host cells.

Bibliography

Zimmer, T., & Schunck, W. (1995). A deviation from the universal genetic code in Candida maltosa and consequences for heterologous expression of cytochromes P450 52A4 and 52A5 in Saccharomyces cerevisiae. Yeast, 11(1), 33â41. Portico.

Authors 2

Thomas Zimmer (first)
Wolf‐Hagen Schunck (additional)

References 30 Referenced 15

10.1016/B978-0-12-372380-2.50009-3
10.1016/S0021-9258(18)50681-5 / J. Biol. Chem. / The preparation and characterization of a cell‐free system from Saccharomyces cerevisiae that translates natural messenger ribonucleic acid by Gasior E. (1979)
10.1073/pnas.79.19.5818
{'key': 'e_1_2_1_5_1', 'first-page': '109', 'volume-title': 'DNA Cloning I', 'author': 'Hanahan D.', 'year': '1985'} / DNA Cloning I by Hanahan D. (1985)
10.1007/978-1-349-20235-5_6
{'key': 'e_1_2_1_7_1', 'first-page': '596', 'volume-title': 'Methods in Enzymology', 'author': 'Holley R. W.', 'year': '1967'} / Methods in Enzymology by Holley R. W. (1967)
10.1038/341164a0
10.1007/BF00365751
10.1016/S0021-9258(19)52451-6
10.1089/dna.1993.12.1
10.1093/nar/21.17.4039
10.1271/bbb1961.55.1757
10.1089/dna.1991.10.271
10.1271/bbb.57.1793
10.1016/S0021-9258(20)82244-3
10.1128/mr.56.1.229-264.1992
{'key': 'e_1_2_1_18_1', 'volume-title': 'Molecular Cloning: A Laboratory Manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular Cloning: A Laboratory Manual by Sambrook J. (1989)
10.1073/pnas.74.12.5463
10.1016/0378-1119(89)90014-0
10.1007/BF00313076
10.1002/j.1460-2075.1993.tb05693.x
10.1016/0006-291X(89)92677-6
10.1007/BF00463482
{'key': 'e_1_2_1_25_1', 'first-page': '336', 'article-title': 'Comparison of two cytochromes P450 from Candida maltosa: primary structure, substrate specificities and effects of their expression in Saccharomyces cerevisiae on the proliferation of the endoplasmic reticulum', 'volume': '55', 'author': 'Schunck W.‐H.', 'year': '1991', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. / Comparison of two cytochromes P450 from Candida maltosa: primary structure, substrate specificities and effects of their expression in Saccharomyces cerevisiae on the proliferation of the endoplasmic reticulum by Schunck W.‐H. (1991)
10.1089/dna.1992.11.767
10.1002/yea.320110106
10.1271/bbb1961.53.2217
10.1016/0006-291X(86)90454-7
10.1002/j.1460-2075.1988.tb03006.x
10.1073/pnas.89.16.7408

Dates

Type	When
Created	20 years, 2 months ago (May 28, 2005, 7:52 p.m.)
Deposited	1 year, 9 months ago (Oct. 26, 2023, 2:55 p.m.)
Indexed	1 year, 9 months ago (Oct. 27, 2023, 4:45 a.m.)
Issued	30 years, 7 months ago (Jan. 1, 1995)
Published	30 years, 7 months ago (Jan. 1, 1995)
Published Online	21 years, 6 months ago (Jan. 29, 2004)
Published Print	30 years, 7 months ago (Jan. 1, 1995)

Funders 0

None

BibTeX

@article{Zimmer_1995, title={A deviation from the universal genetic code in Candida maltosa and consequences for heterologous expression of cytochromes P450 52A4 and 52A5 in Saccharomyces cerevisiae}, volume={11}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.320110105}, DOI={10.1002/yea.320110105}, number={1}, journal={Yeast}, publisher={Wiley}, author={Zimmer, Thomas and Schunck, Wolf‐Hagen}, year={1995}, month=jan, pages={33–41} }

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