DOI: 10.1002/yea.320090410. Targeting of a heterologous protein to the cell wall of <i>Saccharomyces cerevisiae</i>

Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae

10.1002/yea.320090410

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractThe sexual adhesion protein of Saccharomyces cerevisiae MATα cells, α‐agglutinin, could not be extracted from the cell wall with hot sodium dodecyl sulfate (SDS), but became soluble after digestion of the cell with laminarinase. This indicates that it is intimately associated with cell wall glucan. A fusion protein was constructed consisting of the signal sequence of yeast invertase, guar α‐galactosidase, and the C‐terminal half of the α‐agglutinin. Most of the fusion protein was incorporated in the cell wall. A small amount could be extracted with SDS, but most of it could only be extracted with laminarinase. On the other hand, cells containing a construct consisting of the signal sequence of invertase and α‐galactosidase released most of the α‐galactosidase into the medium and all cell wall‐associated α‐galactosidase was released by SDS. Labelling with antibodies showed that the α‐galactosidase part of the fusion protein was exposed on the surface of the cell wall. The results demonstrate that the C‐terminal half of the α‐agglutinin contains the information needed to incorporate a protein into the cell wall.

Bibliography

Schreuder, M. P., Brekelmans, S., Van Den Ende, H., & Klis, F. M. (1993). Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae. Yeast, 9(4), 399â409. Portico.

Authors 4

Maarten P. Schreuder (first)
Stephan Brekelmans (additional)
Herman Van Den Ende (additional)
Frans M. Klis (additional)

References 38 Referenced 123

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{'key': 'e_1_2_1_28_1', 'first-page': '4196', 'article-title': 'The AGA1 product is involved in cell surface attachment of the Saccharomyces cerevisiae cell adhesion glycoprotein a‐agglutinin', 'volume': '11', 'author': 'Roy A.', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The AGA1 product is involved in cell surface attachment of the Saccharomyces cerevisiae cell adhesion glycoprotein a‐agglutinin by Roy A. (1991)
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10.1128/jb.156.2.552-558.1983 / J. Bacteriol. / Isolation of mannan‐protein complexes from viable cells of Saccharomyces cerevisiae X2180‐1A wild type and Saccharomyces cerevisiae X2180‐1A‐5 mutant strains by the action of Zymolyase 60,000 by Shibata N. (1983)
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Wojciechowicz D.(1991). The immunological and molecular characterization of α‐agglutinin fromSaccharomyces cerevisiae. PhD thesis City University of New York New York USA.

Dates

Type	When
Created	20 years, 3 months ago (May 27, 2005, 9 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 1:09 a.m.)
Indexed	4 weeks ago (Aug. 7, 2025, 4:54 a.m.)
Issued	32 years, 5 months ago (April 1, 1993)
Published	32 years, 5 months ago (April 1, 1993)
Published Online	21 years, 7 months ago (Jan. 29, 2004)
Published Print	32 years, 5 months ago (April 1, 1993)

Funders 0

None

BibTeX

@article{Schreuder_1993, title={Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae}, volume={9}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.320090410}, DOI={10.1002/yea.320090410}, number={4}, journal={Yeast}, publisher={Wiley}, author={Schreuder, Maarten P. and Brekelmans, Stephan and Van Den Ende, Herman and Klis, Frans M.}, year={1993}, month=apr, pages={399–409} }

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