DOI: 10.1002/yea.320070907. Molecular cloning of the γ‐glutamylcysteine synthetase gene of <i>Saccharomyces cerevisiae</i>

Molecular cloning of the γ‐glutamylcysteine synthetase gene of Saccharomyces cerevisiae

10.1002/yea.320070907

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractThe 4·4 kb SphI DNA fragment (GSH1) that complements the γ‐glutamylcysteine synthetase‐deficient mutation (gsh1) of Saccharomyces cerevisiae YH1 was cloned into vector plasmid YEp24. Gene disruption of the cloned fragment confirmed that this segment was the same gene as gsh1. Mutant strain YH1 with this plasmid not only restored γ‐glutamylcysteine synthetase (GSH‐1) activity but the glutathione content and the growth rate. DNA sequence analysis of the SphI fragment showed that the GSH1 structural gene contained 2034 bp and predicted a polypeptide of 678 amino acids. The deduced amino acid sequence had about a 45% homology to that of rat kidney GSH‐I, but a very low homology (about 26%) to that of Escherichia coli GSH‐I. Northern analysis showed that GSH1 had been transcribed into an approximately 2·7 kb mRNA fragment. Southern analysis showed that GSH1 mapped at chromosome X.

Bibliography

Ohtake, Y., & Yabuuchi, S. (1991). Molecular cloning of the Î³âglutamylcysteine synthetase gene of Saccharomyces cerevisiae. Yeast, 7(9), 953â961. Portico.

Authors 2

Yasuyuki Ohtake (first)
Seizou Yabuuchi (additional)

References 29 Referenced 105

10.1016/0304-4165(75)90206-8
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10.1016/S0021-9258(19)40057-4 / J. Biol. Chem. / Amino acid sequence of rat kidney γ‐glutamylcysteine synthetase by Yan N. (1990)

Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 7:34 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 11:45 p.m.)
Indexed	2 months, 3 weeks ago (June 3, 2025, 12:55 a.m.)
Issued	33 years, 8 months ago (Dec. 1, 1991)
Published	33 years, 8 months ago (Dec. 1, 1991)
Published Online	21 years, 7 months ago (Jan. 29, 2004)
Published Print	33 years, 8 months ago (Dec. 1, 1991)

Funders 0

None

BibTeX

@article{Ohtake_1991, title={Molecular cloning of the γ‐glutamylcysteine synthetase gene of Saccharomyces cerevisiae}, volume={7}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.320070907}, DOI={10.1002/yea.320070907}, number={9}, journal={Yeast}, publisher={Wiley}, author={Ohtake, Yasuyuki and Yabuuchi, Seizou}, year={1991}, month=dec, pages={953–961} }

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