DOI: 10.1002/yea.320070609. A family of low and high copy replicative, integrative and single‐stranded <i>S. cerevisiae</i>/<i>E. coli</i> shuttle vectors

A family of low and high copy replicative, integrative and single‐stranded S. cerevisiae/E. coli shuttle vectors

10.1002/yea.320070609

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractWe describe a set of replicative, integrative and single‐stranded shuttle vectors constructed from the pUC19 plasmid that we use routinely in our experiments. They bear a yeast selectable marker: URA3, TRP1 or LEU2. Replicative vectors carrying different yeast replication origins have been constructed in order to have plasmids based on the same construction with a high or low copy number per cell and with different mitotic stabilities. All the vectors are small in size, provide a high yield in Escherichia coli and efficiently transform Saccharomyces cerevisiae. These plasmids have many of the unique sites of the pUC19 multicloning region and many of them allow for the screening of plasmids with an insert by alpha‐complementation. The nucleotide sequence of each of them is completely known.

Bibliography

Bonneaud, N., OzierâKalogeropoulos, O., Li, G., Labouesse, M., MinvielleâSebastia, L., & Lacroute, F. (1991). A family of low and high copy replicative, integrative and singleâstranded S. cerevisiae/E. coli shuttle vectors. Yeast, 7(6), 609â615. Portico.

Authors 6

Nathalie Bonneaud (first)
Odile Ozier‐Kalogeropoulos (additional)
Guoya Li (additional)
Michel Labouesse (additional)
Lionel Minvielle‐Sebastia (additional)
Francois Lacroute (additional)

References 42 Referenced 411

10.1073/pnas.76.1.386
10.1016/0378-1119(85)90154-4
10.1093/genetics/117.4.645 / Genetics / Identifying mutations in duplicated functions in Saccharomyces cerevisiae: Recessive mutations in HMG‐CoA reductase genes by Basson M. E. (1987)
10.1007/BF00269900
10.1007/BF00330984
10.1016/0378-1119(79)90012-X
10.1016/0092-8674(80)90487-0
10.1101/SQB.1983.047.01.132
10.1007/978-1-4684-5377-5_9
10.1016/0378-1119(80)90082-7
10.1038/287504a0
10.1007/BF00312853
10.1093/nar/11.6.1645
10.1016/S0022-2836(84)80020-0
10.1016/0378-1119(88)90202-8
10.1128/jb.157.1.283-290.1984 / J. Bacteriol. / Copy number and the stability of 2‐μm circle based artificial plasmids of Saccharomyces cerevisiae by Futcher A. B. (1984)
10.1016/0378-1119(88)90185-0
10.1038/286860a0
Herbert C. J. Bécam A. M. Li G.‐Y. Dujardin G.andSlonimski P. P.(1991). Nuclear and mitochondrial proteins involved in pre‐mRNA splicing in yeast mitochondria.J. of Wroclaw University in press.
10.1007/BF00339595
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10.1016/S0021-9258(19)39111-2 / J. Biol. Chem. / The ferrochelatase from Saccharomyces cerevisiae by Labbe‐Bois R. (1990)
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10.1111/j.1475-4754.1982.tb01001.x
10.1016/0092-8674(87)90241-8
10.1093/nar/16.5.1829
Minivielle‐Sebastia L. Winsor B. Bonneaud N.andLacroute F.(1991). Mutations in the yeastRNA14andRNA15genes induce poly(A) tail shortening. Sequence analysis reveals an RNA binding domain in theRNA15protein. Submitted. (10.1128/MCB.11.6.3075)
{'key': 'e_1_2_1_33_1', 'first-page': 'S328', 'article-title': 'Two genes are responsible for CTP biosynthesis in Saccharomyces cerevisiae', 'volume': '6', 'author': 'Ozier‐Kalogeropoulos O.', 'year': '1990', 'journal-title': 'Yeast'} / Yeast / Two genes are responsible for CTP biosynthesis in Saccharomyces cerevisiae by Ozier‐Kalogeropoulos O. (1990)
10.1002/j.1460-2075.1982.tb01362.x
Renault G.(1990). Approache génétique du rǒle fonctionnel de la méthylation de la coiffe del' ARN messager chezSaccharomyces cerevisiae. Thèse de Doctorat Facultés Universitaires N. D. de la Paix Namur Belgique.
Rose M. D.andBroach J. R.(1991). Cloning genes by complementation in yeast. In Guthrie C. and Fink G. R. (Eds) Methods in Enzymology vol. 194 pp.195–230. (10.1016/0076-6879(91)94017-7)
10.1126/science.7046050
10.1093/genetics/122.1.19
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10.1016/0022-2836(67)90341-5
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10.1016/0378-1119(85)90120-9
10.1016/S0021-9258(19)81577-6 / J. Biol. Chem. / Isolation, sequence and regulation by oxygen of the yeast HEM13 gene coding for coproporphyrinogen oxidase by Zagorec M. (1988)

Dates

Type	When
Created	20 years, 2 months ago (May 28, 2005, 7:32 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 11:58 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 7, 2025, 5:04 p.m.)
Issued	34 years ago (Aug. 1, 1991)
Published	34 years ago (Aug. 1, 1991)
Published Online	21 years, 6 months ago (Jan. 29, 2004)
Published Print	34 years ago (Aug. 1, 1991)

Funders 0

None

BibTeX

@article{Bonneaud_1991, title={A family of low and high copy replicative, integrative and single‐stranded S. cerevisiae/E. coli shuttle vectors}, volume={7}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.320070609}, DOI={10.1002/yea.320070609}, number={6}, journal={Yeast}, publisher={Wiley}, author={Bonneaud, Nathalie and Ozier‐Kalogeropoulos, Odile and Li, Guoya and Labouesse, Michel and Minvielle‐Sebastia, Lionel and Lacroute, Francois}, year={1991}, month=aug, pages={609–615} }

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