DOI: 10.1002/yea.320050304. Structure and nuclear localization signal of the SK13 antiviral protein of <i>Saccharomyces cerevisiae</i>

Structure and nuclear localization signal of the SK13 antiviral protein of Saccharomyces cerevisiae

10.1002/yea.320050304

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractThe yeast chromosomal genes SK12, SK13, SK14, SK16, SK17 and SK18 repress the replication of double‐stranded RNA viruses, protecting the host from the otherwise lethal effects of the virus. We cloned and sequenced the SK13 gene and found that it encodes a 163 kDa protein including a typical nuclear localization signal. Cell fractionation experiments show that the SK13 gene product is indeed tightly associated with nuclei and that the putative nuclear localization sequence directs β‐galactosidase into the nucleus. However, fusion of a part of the SK13 protein lacking this signal with β‐galactosidase directs β‐galactosidase into the nucleus, suggesting the presence of a second nuclear localization signal. The SK13 gene is only essential in the presence of an M double‐stranded RNA virus.

Bibliography

Rhee, S., Icho, T., & Wickner, R. B. (1989). Structure and nuclear localization signal of the SK13 antiviral protein of Saccharomyces cerevisiae. Yeast, 5(3), 149â158. Portico.

Authors 3

Sang‐Ki Rhee (first)
Tateo Icho (additional)
Reed B. Wickner (additional)

References 33 Referenced 48

10.1002/yea.320040103
10.1016/S0021-9258(17)30351-4 / J. Biol. Chem. / Spermidine or spermine requirement for the killer double‐stranded RNA plasmid replication in yeast by Cohn M. S. (1978)
10.1002/j.1460-2075.1987.tb04813.x
10.1093/genetics/117.3.399 / Genetics / A new non‐Mendelian genetic element of yeast that increases cytopathology produced by M1 double‐stranded RNA in ski strains by Esteban R. (1987)
10.1016/0092-8674(88)90225-5
10.1073/pnas.78.4.2199
10.1128/MCB.6.11.3990
10.1016/S0021-9258(19)57326-4 / J. Biol. Chem. / The MAK11 protein is essential for cell growth and replication of M double‐stranded RNA and is apparently a membrane‐associated protein by Icho T. (1988)
Icho T.andWickner R. B.(1989). The double‐stranded RNA genome of yeast virus L‐A encodes its own putative RNA polymerase by fusing two open reading frames.J. Biol. Chem. in press. (10.1016/S0021-9258(18)83488-3)
10.1038/331280a0
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10.1093/genetics/100.3.387 / Genetics / A new meiotic mapping method employing a meiotic rec − mutant of yeast by Klapholz S. (1982)
10.1016/S0091-679X(08)60325-8
10.1002/j.1460-2075.1985.tb03766.x
10.1016/0378-1119(86)90028-4
10.1146/annurev.bi.56.070187.002535
10.1128/jb.130.3.1303-1309.1977 / J. Bacteriol. / Biochemical and physiological studies of the yeast virus‐like particle by Oliver S. G. (1977)
10.1128/MCB.4.4.761
10.1016/0076-6879(83)01015-0
10.1073/pnas.81.19.5951
10.1016/0042-6822(87)90338-2
10.1002/j.1460-2075.1984.tb01988.x
10.1073/pnas.84.14.4767
10.1073/pnas.82.13.4374
10.1016/S0021-9258(17)43412-0 / J. Biol. Chem. / Identification of Saccharomyces cerevisiae mutants deficient in DNA topoisomerase I activity by Thrash C. (1984)
10.1128/jb.136.3.1002-1007.1978
10.1002/yea.320010204
10.1073/pnas.77.1.527
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10.1016/0092-8674(80)90129-4
10.1128/jb.169.11.4941-4945.1987 / J. Bacteriol. / MKT1, a nonessential M2 gene with a temperature‐dependent effect on replication of M2 double‐stranded RNA by Wickner R. B. (1987)
10.1073/pnas.85.16.6007
10.1073/pnas.79.15.4706

Dates

Type	When
Created	20 years, 2 months ago (May 28, 2005, 7:24 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 12:02 a.m.)
Indexed	3 weeks ago (Aug. 5, 2025, 8:43 a.m.)
Issued	36 years, 3 months ago (May 1, 1989)
Published	36 years, 3 months ago (May 1, 1989)
Published Online	21 years, 6 months ago (Jan. 29, 2004)
Published Print	36 years, 3 months ago (May 1, 1989)

Funders 0

None

BibTeX

@article{Rhee_1989, title={Structure and nuclear localization signal of the SK13 antiviral protein of Saccharomyces cerevisiae}, volume={5}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.320050304}, DOI={10.1002/yea.320050304}, number={3}, journal={Yeast}, publisher={Wiley}, author={Rhee, Sang‐Ki and Icho, Tateo and Wickner, Reed B.}, year={1989}, month=may, pages={149–158} }

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