DOI: 10.1002/yea.320050108. Sequence and mutational analysis of <i>ESS1</i>, a gene essential for growth in <i>Saccharomyces cerevisiae</i>

Sequence and mutational analysis of ESS1, a gene essential for growth in Saccharomyces cerevisiae

10.1002/yea.320050108

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractA newly isolated gene, ESS1, was shown to encode a protein required for vegetative growth in Saccharomyces cerevisiae. The nucleotide sequence of ESS1 revealed a 172 amino acid open reading frame predicting a highly basic, 19·5 kilodalton product. Although the gene was isolated by cross‐hybridization with the vertebrate v‐sis oncogene, the primary amino acid sequence bears only a slight resemblance to the p28sis protein. ESS1 was shown to be single copy in the yeast genome and transcriptionally active during logarithmic growth. It is located on the right arm of chromosome X, 6 centimorgans distal to ilv3. The genetic map location indicates it is not allelic to any previously characterized mutation in this organism. Both inactivation of ESS1 by gene disruption and overexpression by fusion to a heterologous promoter were detrimental to growth in both haploid and diploid cell types. Under non‐permissive conditions, the terminal phenotype of strains containing a suppressible amber mutation within ESS1 was one of aberrant multibudded structures. Examination of this morphology indicates that loss of ESS1 function may lead to a defect in cytokinesis or cell separation.

Bibliography

Hanes, S. D., Shank, P. R., & Bostian, K. A. (1989). Sequence and mutational analysis of ESS1, a gene essential for growth in Saccharomyces cerevisiae. Yeast, 5(1), 55â72. Portico.

Authors 3

Steven D. Hanes (first)
Peter R. Shank (additional)
Keith A. Bostian (additional)

References 48 Referenced 141

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{'key': 'e_1_2_1_14_1', 'volume-title': 'Of URFS and ORFS: A Primer on how to Analyze Derived Amino Acid Sequences', 'author': 'Doolittle R. F.', 'year': '1986'} / Of URFS and ORFS: A Primer on how to Analyze Derived Amino Acid Sequences by Doolittle R. F. (1986)
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{'key': 'e_1_2_1_39_1', 'volume-title': 'Methods in Yeast Genetics', 'author': 'Sherman F.', 'year': '1978'} / Methods in Yeast Genetics by Sherman F. (1978)
{'key': 'e_1_2_1_40_1', 'volume-title': 'Methods in Yeast Genetics', 'author': 'Sherman F.', 'year': '1986'} / Methods in Yeast Genetics by Sherman F. (1986)
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{'key': 'e_1_2_1_46_1', 'first-page': '158', 'article-title': 'Tightly centromere‐linked gene (SPO15) essential for meiosis in the yeast Saccharomyces cerevisiae', 'volume': '6', 'author': 'Yeh E.', 'year': '1986', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Tightly centromere‐linked gene (SPO15) essential for meiosis in the yeast Saccharomyces cerevisiae by Yeh E. (1986)
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Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 7:24 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 12:03 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 6, 2025, 9:18 a.m.)
Issued	36 years, 7 months ago (Jan. 1, 1989)
Published	36 years, 7 months ago (Jan. 1, 1989)
Published Online	21 years, 7 months ago (Jan. 29, 2004)
Published Print	36 years, 7 months ago (Jan. 1, 1989)

Funders 0

None

BibTeX

@article{Hanes_1989, title={Sequence and mutational analysis of ESS1, a gene essential for growth in Saccharomyces cerevisiae}, volume={5}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.320050108}, DOI={10.1002/yea.320050108}, number={1}, journal={Yeast}, publisher={Wiley}, author={Hanes, Steven D. and Shank, Peter R. and Bostian, Keith A.}, year={1989}, month=jan, pages={55–72} }

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