DOI: 10.1002/yea.320030108. Molecular cloning of chromosome I DNA from <i>Saccharomyces cerevisiae</i>: Isolation of the <i>MAK16</i> gene and analysis of an adjacent gene essential for growth at low temperatures

Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: Isolation of the MAK16 gene and analysis of an adjacent gene essential for growth at low temperatures

10.1002/yea.320030108

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractMAK16 is an essential gene on chromosome I defined by the thermosensitive lethal mak16–1 mutation. MAK16 is also necessary for M double‐stranded RNA replication at the permissive temperature for cell growth. As part of an effort to clone all the DNA from chromosome I, plasmids that complemented both the temperature‐sensitive growth defect, and the M1 replication defects of mak16–1 strains were isolated from a plasmid YCp50: Saccharomyces cerevisiae recombinant DNA library. The two plasmids analysed contained overlapping inserts that hybridized proportionally to strains carrying different dosages of chromosome I. Furthermore, integration of a fragment of one of these clones occurred at a site linked to ade1, confirming that this clone was derived from the appropriate region of chromosome I. An open reading frame adjacent to MAK16 potentially coding for a 468 amino acid protein was defined by sequence analysis. 185 amino acids of this open reading frame were replaced with a 1·2 kb fragment carrying the S. cerevisiae URA3 gene by a one‐step gene disruption. The resulting strains grew at a rate indistinguishable from the wild type at 20°C, 30°C, or 37°C, but could not grow at 8°C. The deleted region is thus essential only at 8°C, and we name this gene LTE1 (low temperature essential).

Bibliography

Wickner, R. B., Koh, T. J., Crowley, J. C., OâNeil, J., & Kaback, D. B. (1987). Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: Isolation of the MAK16 gene and analysis of an adjacent gene essential for growth at low temperatures. Yeast, 3(1), 51â57. Portico.

Authors 5

Reed B. Wickner (first)
Theodore J. Koh (additional)
Joan C. Crowley (additional)
Judy O'Neil (additional)
David B. Kaback (additional)

References 21 Referenced 46

10.1073/pnas.82.11.3756
Coleman K. G. Steensma H. Y. Kaback D. B.andPringle J. R.(1987). Molecular cloning of chromosome I DNA fromSaccharomyces cerevisiae:isolation and characterization of theCDC24gene and adjacent regions of the chromosome.Mol. Cell. Biol. in press. (10.1128/MCB.6.12.4516)
10.1128/jb.159.1.413-417.1984 / J. Bacteriol. / Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation of the ADE1 gene by Crowley J. C. (1984)
10.1016/0378-1119(84)90153-7
10.1128/jb.153.1.163-168.1983
10.1093/nar/6.7.2499
{'key': 'e_1_2_1_8_1', 'volume-title': 'Molecular Cloning—a Laboratory Manual', 'author': 'Maniatis T.', 'year': '1982'} / Molecular Cloning—a Laboratory Manual by Maniatis T. (1982)
10.1016/S0091-679X(08)60325-8
10.1128/mr.49.3.181-213.1985 / Microbiol Rev. / Genetic map of Saccharomyces cerevisiae, edition 9 by Mortimer R. K. (1985)
10.1128/MCB.4.4.761
10.1016/0076-6879(83)01015-0
10.1073/pnas.74.12.5463
10.1016/0092-8674(84)90301-5
10.1016/0022-2836(69)90474-4
10.1093/genetics/77.4.651
10.1016/S0022-2836(75)80083-0
10.1128/jb.136.3.1002-1007.1978
10.1002/yea.320010204
10.1093/genetics/88.3.419 / Genetics / Twenty‐six chromosomal genes needed to maintain the killer double‐stranded RNA plasmid of Saccharomyces cerevisiae by Wickner R. B. (1978)
10.1128/jb.140.1.154-160.1979 / J. Bacteriol. / mak mutants of yeast: mapping and characterization by Wickner R. B. (1979)
10.1073/pnas.80.3.726

Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 7:21 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 6:12 a.m.)
Indexed	1 year, 6 months ago (Feb. 16, 2024, 7:31 p.m.)
Issued	38 years, 6 months ago (March 1, 1987)
Published	38 years, 6 months ago (March 1, 1987)
Published Online	21 years, 7 months ago (Jan. 29, 2004)
Published Print	38 years, 6 months ago (March 1, 1987)

Funders 0

None

BibTeX

@article{Wickner_1987, title={Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: Isolation of the MAK16 gene and analysis of an adjacent gene essential for growth at low temperatures}, volume={3}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.320030108}, DOI={10.1002/yea.320030108}, number={1}, journal={Yeast}, publisher={Wiley}, author={Wickner, Reed B. and Koh, Theodore J. and Crowley, Joan C. and O’Neil, Judy and Kaback, David B.}, year={1987}, month=mar, pages={51–57} }

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