DOI: 10.1002/yea.320010108. Primary structure of the <i>Saccharomyces cerevisiae GAL7</i> gene

Primary structure of the Saccharomyces cerevisiae GAL7 gene

10.1002/yea.320010108

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractWe present the nucleotide sequence of a 1599‐base pair (bp) DNA fragment containing the entire GAL7 gene that encodes galactose‐1‐phosphate uridyltransferase of Saccharomyces cerevisiae. The deduced peptide was composed of 364 amino acid residues. The expected molecular weight was 42 005 daltons, which agreed with the observed value for the purified enzyme.1 The 3′‐end of the GAL7 transcript mapped at a position 82 bp downstream from the UAA termination codon by the S1 nuclease protection experiment. We constructed a GAL7′‐lac′Z fusion on various types of yeast plasmid vectors. The fused gene on any type of vector was induced by galactose and repressed by glucose as for the GAL7 gene on the chromosome. The response of GAL7′‐lac′Z fusion to gal4Δ and gal80Δ regulatory mutations was also similar to the response of the chromosomal GAL7 gene. By using various deletions in the 5′‐flanking region of the gene fusion, we delimited the sequence essential for galactose controlled expression with a 180 bp‐fragment of DNA lying 92 bp upstream of the transcription initiation site.

Bibliography

Tajima, M., Nogi, Y., & Fukasawa, T. (1985). Primary structure of the Saccharomyces cerevisiae GAL7 gene. Yeast, 1(1), 67â77. Portico.

Authors 3

Masahiro Tajima (first)
Yasuhisa Nogi (additional)
Toshio Fukasawa (additional)

References 46 Referenced 112

10.1016/S0021-9258(19)86578-X / The Journal of Biological Chemistry / The enzymes of the galactose cluster in Saccharomyces cerevisiae: Purification and characterization of galactose‐1‐phosphate uridylyltransferase by Segawa T. (1979)
10.1093/genetics/49.5.837 / Genetics / Enzymatic expression and genetic linkage of genes controlling galactose utilization in Saccharomyces cerevisiae by Douglas H. C. (1964)
10.1016/0022-2836(81)90244-8
10.1093/genetics/54.3.911
10.1016/0092-8674(79)90020-5
10.1128/jb.125.1.33-41.1976 / Journal of Bacteriology / Genetic coregulation of galactose and melibiose utilization in Saccharomyces cerevisiae by Kew O. H. (1976)
10.1007/BF00420623
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10.1073/pnas.79.22.6827
{'key': 'e_1_2_1_11_2', 'first-page': '260', 'article-title': 'Primary structure of the Saccharomyces cerevisiae GAL4 gene', 'volume': '4', 'author': 'Laughton A.', 'year': '1984', 'journal-title': 'Molecular and Cellular Biology'} / Molecular and Cellular Biology / Primary structure of the Saccharomyces cerevisiae GAL4 gene by Laughton A. (1984)
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{'key': 'e_1_2_1_18_2', 'first-page': '159', 'volume-title': 'The Molecular Biology of the Yeast Saccharomyces', 'author': 'Oshima Y.', 'year': '1982'} / The Molecular Biology of the Yeast Saccharomyces by Oshima Y. (1982)
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{'key': 'e_1_2_1_33_2', 'volume-title': 'Molecular Cloning: a Laboratory Manual', 'author': 'Maniatis T.', 'year': '1982'} / Molecular Cloning: a Laboratory Manual by Maniatis T. (1982)
10.1016/S0091-679X(08)62009-9
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{'key': 'e_1_2_1_37_2', 'first-page': '352', 'volume-title': 'Experiments in Molecular Genetics: Experiment 48 Assay of β‐galactosidase', 'author': 'Miller J. H.', 'year': '1972'} / Experiments in Molecular Genetics: Experiment 48 Assay of β‐galactosidase by Miller J. H. (1972)
10.1016/S0021-9258(19)52451-6
10.1128/jb.143.2.971-980.1980 / Journal of Bacteriology / In vitro gene fusion that join an enzymatically active β‐galactosidase segment to amino‐terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals by Casadaban M. J. (1980)
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10.1016/S0021-9258(19)81068-2 / The Journal of Biology Chemistry / Codon selection in yeast by Bennetzen J. L. (1982)
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10.1093/nar/12.23.8951

Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 5:50 p.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 9:35 p.m.)
Indexed	1 year ago (Aug. 8, 2024, 3:55 a.m.)
Issued	40 years ago (Sept. 1, 1985)
Published	40 years ago (Sept. 1, 1985)
Published Online	21 years, 7 months ago (Jan. 29, 2004)
Published Print	40 years ago (Sept. 1, 1985)

Funders 0

None

BibTeX

@article{Tajima_1985, title={Primary structure of the Saccharomyces cerevisiae GAL7 gene}, volume={1}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.320010108}, DOI={10.1002/yea.320010108}, number={1}, journal={Yeast}, publisher={Wiley}, author={Tajima, Masahiro and Nogi, Yasuhisa and Fukasawa, Toshio}, year={1985}, month=sep, pages={67–77} }

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