DOI: 10.1002/yea.1488. Tools and methods for genetic analysis of <i>Saccharomyces castellii</i>

Tools and methods for genetic analysis of Saccharomyces castellii

10.1002/yea.1488

Crossref journal-article

Wiley

Yeast (311)

Abstract

AbstractThe budding yeast species Saccharomyces castellii has provided important new insights into molecular evolution when incorporated in comparative genomics studies and studies of mitochondrial inheritage. Although it shows some diversity in the specific molecular details, several analyses have shown that it contains many genetic pathways similar to those of S. cerevisiae. Here we have investigated the possibility of performing genetic analyses in S. castellii. We optimized the LiAc transformation protocol to achieve 200–300 transformants/µg plasmid DNA. We found that the commonly used plasmids for S. cerevisiae are stably maintained in S. castellii under selective conditions. Surprisingly, both 2µ and CEN/ARS plasmids are kept at a high copy number. Moreover, the kanMX cassette can be used as a resistance marker against the selective drug geneticin (G418). Finally, we determined that the S. cerevisiae GAL1 promoter can be used for the activation of transcription in S. castellii, thus enabling the controlled overexpression of genes when galactose is present in the medium. The availability of these tools provides the possibility of performing genetic analyses in S. castellii, and makes it a promising new model system in which hypotheses derived from bioinformatics studies can be experimentally tested. Copyright © 2007 John Wiley & Sons, Ltd.

Bibliography

Astromskas, E., & Cohn, M. (2007). Tools and methods for genetic analysis of Saccharomyces castellii. Yeast, 24(6), 499â509. Portico.

Authors 2

Eimantas Astromskas (first)
Marita Cohn (additional)

References 35 Referenced 12

10.1002/yea.1413
10.1073/pnas.0304170101
10.1002/(SICI)1097-0061(19970915)13:11<1065::AID-YEA159>3.0.CO;2-K
10.1126/science.1084337
10.1534/genetics.105.048900
10.1101/gr.182901
10.1126/science.7618104
10.1007/s002940050312
10.1016/0378-1119(85)90252-5
10.2144/01304rv02
10.1016/S0076-6879(02)50957-5
{'key': 'e_1_2_5_13_1', 'first-page': '107', 'article-title': 'Yeast transformation by the LiAc/SS Carrier DNA/PEG method', 'volume': '313', 'author': 'Gietz RD', 'year': '2006', 'journal-title': 'Methods Mol Biol'} / Methods Mol Biol / Yeast transformation by the LiAc/SS Carrier DNA/PEG method by Gietz RD (2006)
10.1002/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K
10.1074/jbc.M608727200
10.1128/mr.51.4.458-476.1987
10.1093/nar/gkl064
10.1016/S1567-1356(03)00175-2
10.1093/nar/gkg423
10.1002/1097-0290(20001020)70:2<187::AID-BIT8>3.0.CO;2-H
10.1002/bies.20259
10.1002/bies.20093
10.1128/JB.181.20.6488-6496.1999
{'key': 'e_1_2_5_24_1', 'first-page': '565', 'article-title': '(Genetic analysis of Saccharomyces castellii, S. exiguus and S. martiniae yeasts)', 'volume': '34', 'author': 'Naumov GI', 'year': '1998', 'journal-title': 'Genetika'} / Genetika / (Genetic analysis of Saccharomyces castellii, S. exiguus and S. martiniae yeasts) by Naumov GI (1998)
10.1016/0022-2836(81)90438-1
10.1016/S0022-2836(02)00037-2
10.1099/00207713-49-4-1925
10.1016/j.jmb.2005.10.078
10.1016/0076-6879(91)94028-B
10.1093/genetics/122.1.19
10.1016/S1567-1356(02)00204-0
10.1002/yea.1173
10.1002/yea.320101310
10.1002/yea.816
10.1080/20013891081719
10.1098/rstb.2005.1799

Dates

Type	When
Created	18 years, 4 months ago (April 13, 2007, 5:28 a.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 6:24 p.m.)
Indexed	1 year, 7 months ago (Jan. 10, 2024, 3:45 a.m.)
Issued	18 years, 4 months ago (April 13, 2007)
Published	18 years, 4 months ago (April 13, 2007)
Published Online	18 years, 4 months ago (April 13, 2007)
Published Print	18 years, 2 months ago (June 1, 2007)

Funders 0

None

BibTeX

@article{Astromskas_2007, title={Tools and methods for genetic analysis of Saccharomyces castellii}, volume={24}, ISSN={1097-0061}, url={http://dx.doi.org/10.1002/yea.1488}, DOI={10.1002/yea.1488}, number={6}, journal={Yeast}, publisher={Wiley}, author={Astromskas, Eimantas and Cohn, Marita}, year={2007}, month=apr, pages={499–509} }

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