DOI: 10.1002/smll.201101193. Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells

Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells

10.1002/smll.201101193

Crossref journal-article

Wiley

Small (311)

Abstract

AbstractThe ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in‐vivo anticancer nucleic‐acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs’ intrinsic fluorescence from embedded color‐center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli‐1 gene expression is shown at the mRNA and protein level by the ND‐vectorized siRNA in a serum‐containing medium.

Bibliography

Alhaddad, A., Adam, M., Botsoa, J., Dantelle, G., Perruchas, S., Gacoin, T., Mansuy, C., Lavielle, S., Malvy, C., Treussart, F., & Bertrand, J. (2011). Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells. Small, 7(21), 3087â3095. Portico.

Authors 11

Anna Alhaddad (first)
Marie‐Pierre Adam (additional)
Jacques Botsoa (additional)
Géraldine Dantelle (additional)
Sandrine Perruchas (additional)
Thierry Gacoin (additional)
Christelle Mansuy (additional)
Solange Lavielle (additional)
Claude Malvy (additional)
François Treussart (additional)
Jean‐Rémi Bertrand (additional)

References 48 Referenced 204

10.1016/j.ijpharm.2010.03.063
10.1016/S0169-409X(03)00108-X
10.1016/S0169-409X(98)00048-9 / Adv. Drug Deliv. Rev. by Pouton C. (1998)
10.1023/A:1016017014573
10.5732/cjc.010.10075
10.1021/nl071521o
10.1007/s12274-009-9009-8
10.1021/nn900865g
10.1016/j.biomaterials.2009.04.041
10.1016/j.biomaterials.2009.11.044
10.1038/nmat2608
10.1021/nn102055s
10.1016/j.jconrel.2010.01.008
10.1039/b908532g
10.1080/10408430902831987
10.2217/17435889.4.1.47
10.1088/0957-4484/20/23/235602
10.1038/nnano.2008.99
10.1016/j.jlumin.2009.12.003
10.2971/jeos.2009.09035
10.1021/nl1021909
10.1021/jp066387v
10.1016/j.diamond.2008.10.031
10.1016/j.diamond.2009.11.022
10.1002/smll.200902305
10.1126/scitranslmed.3001713
10.1021/jz1013278
10.1111/j.1476-5381.2009.00148.x
10.1200/JCO.1996.14.4.1245
10.1177/030089160809400218
10.1089/oli.2006.16.158
10.1172/JCI119152
10.1023/A:1026122914852
{'key': 'e_1_2_7_31_2', 'first-page': '72', 'volume': '1002', 'author': 'Maksimenko A.', 'year': '2003', 'journal-title': 'Therapeut. Oligonucl.'} / Therapeut. Oligonucl. by Maksimenko A. (2003)
10.1093/nar/gkm1094
10.1089/oli.2009.0197
10.1021/nn900918w
10.1002/cbic.200800247
10.1021/nn900339s
10.1385/1-59259-823-4:411
10.1021/es1034188
10.1111/j.1365-2818.2006.01706.x
10.1002/jps.10437
10.1021/bc100010k
10.1016/j.diamond.2004.11.004
10.1016/j.diamond.2005.11.019
10.1016/0003-2697(70)90146-6
10.1103/PhysRevB.82.115449

Dates

Type	When
Created	13 years, 11 months ago (Sept. 12, 2011, 12:04 p.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 3:57 p.m.)
Indexed	4 days, 15 hours ago (Aug. 30, 2025, 1:14 p.m.)
Issued	13 years, 11 months ago (Sept. 12, 2011)
Published	13 years, 11 months ago (Sept. 12, 2011)
Published Online	13 years, 11 months ago (Sept. 12, 2011)
Published Print	13 years, 10 months ago (Nov. 4, 2011)

Funders 0

None

BibTeX

@article{Alhaddad_2011, title={Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells}, volume={7}, ISSN={1613-6829}, url={http://dx.doi.org/10.1002/smll.201101193}, DOI={10.1002/smll.201101193}, number={21}, journal={Small}, publisher={Wiley}, author={Alhaddad, Anna and Adam, Marie‐Pierre and Botsoa, Jacques and Dantelle, Géraldine and Perruchas, Sandrine and Gacoin, Thierry and Mansuy, Christelle and Lavielle, Solange and Malvy, Claude and Treussart, François and Bertrand, Jean‐Rémi}, year={2011}, month=sep, pages={3087–3095} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        30
      ]
    ],
    "date-time": "2025-08-30T17:14:00Z",
    "timestamp": 1756574040496
  },
  "reference-count": 48,
  "publisher": "Wiley",
  "issue": "21",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2011,
            9,
            12
          ]
        ],
        "date-time": "2011-09-12T00:00:00Z",
        "timestamp": 1315785600000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2011,
        11,
        4
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in\u2010vivo anticancer nucleic\u2010acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs\u2019 intrinsic fluorescence from embedded color\u2010center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli\u20101 gene expression is shown at the mRNA and protein level by the ND\u2010vectorized siRNA in a serum\u2010containing medium.</jats:p>",
  "DOI": "10.1002/smll.201101193",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2011,
        9,
        12
      ]
    ],
    "date-time": "2011-09-12T16:04:05Z",
    "timestamp": 1315843445000
  },
  "page": "3087-3095",
  "source": "Crossref",
  "is-referenced-by-count": 204,
  "title": "Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells",
  "prefix": "10.1002",
  "volume": "7",
  "author": [
    {
      "given": "Anna",
      "family": "Alhaddad",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Marie\u2010Pierre",
      "family": "Adam",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jacques",
      "family": "Botsoa",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "G\u00e9raldine",
      "family": "Dantelle",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Sandrine",
      "family": "Perruchas",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Thierry",
      "family": "Gacoin",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Christelle",
      "family": "Mansuy",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Solange",
      "family": "Lavielle",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Claude",
      "family": "Malvy",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Fran\u00e7ois",
      "family": "Treussart",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jean\u2010R\u00e9mi",
      "family": "Bertrand",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2011,
        9,
        12
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_7_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.ijpharm.2010.03.063"
    },
    {
      "key": "e_1_2_7_1_3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0169-409X(03)00108-X"
    },
    {
      "key": "e_1_2_7_1_4",
      "doi-asserted-by": "crossref",
      "first-page": "3",
      "DOI": "10.1016/S0169-409X(98)00048-9",
      "volume": "34",
      "author": "Pouton C.",
      "year": "1998",
      "journal-title": "Adv. Drug Deliv. Rev."
    },
    {
      "key": "e_1_2_7_1_5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1023/A:1016017014573"
    },
    {
      "key": "e_1_2_7_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.5732/cjc.010.10075"
    },
    {
      "key": "e_1_2_7_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nl071521o"
    },
    {
      "key": "e_1_2_7_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s12274-009-9009-8"
    },
    {
      "key": "e_1_2_7_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nn900865g"
    },
    {
      "key": "e_1_2_7_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.biomaterials.2009.04.041"
    },
    {
      "key": "e_1_2_7_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.biomaterials.2009.11.044"
    },
    {
      "key": "e_1_2_7_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmat2608"
    },
    {
      "key": "e_1_2_7_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nn102055s"
    },
    {
      "key": "e_1_2_7_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jconrel.2010.01.008"
    },
    {
      "key": "e_1_2_7_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1039/b908532g"
    },
    {
      "key": "e_1_2_7_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1080/10408430902831987"
    },
    {
      "key": "e_1_2_7_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2217/17435889.4.1.47"
    },
    {
      "key": "e_1_2_7_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1088/0957-4484/20/23/235602"
    },
    {
      "key": "e_1_2_7_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nnano.2008.99"
    },
    {
      "key": "e_1_2_7_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jlumin.2009.12.003"
    },
    {
      "key": "e_1_2_7_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2971/jeos.2009.09035"
    },
    {
      "key": "e_1_2_7_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nl1021909"
    },
    {
      "key": "e_1_2_7_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/jp066387v"
    },
    {
      "key": "e_1_2_7_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.diamond.2008.10.031"
    },
    {
      "key": "e_1_2_7_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.diamond.2009.11.022"
    },
    {
      "key": "e_1_2_7_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/smll.200902305"
    },
    {
      "key": "e_1_2_7_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/scitranslmed.3001713"
    },
    {
      "key": "e_1_2_7_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/jz1013278"
    },
    {
      "key": "e_1_2_7_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1476-5381.2009.00148.x"
    },
    {
      "key": "e_1_2_7_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1200/JCO.1996.14.4.1245"
    },
    {
      "key": "e_1_2_7_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1177/030089160809400218"
    },
    {
      "key": "e_1_2_7_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1089/oli.2006.16.158"
    },
    {
      "key": "e_1_2_7_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1172/JCI119152"
    },
    {
      "key": "e_1_2_7_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1023/A:1026122914852"
    },
    {
      "key": "e_1_2_7_31_2",
      "first-page": "72",
      "volume": "1002",
      "author": "Maksimenko A.",
      "year": "2003",
      "journal-title": "Therapeut. Oligonucl."
    },
    {
      "key": "e_1_2_7_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/gkm1094"
    },
    {
      "key": "e_1_2_7_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1089/oli.2009.0197"
    },
    {
      "key": "e_1_2_7_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nn900918w"
    },
    {
      "key": "e_1_2_7_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cbic.200800247"
    },
    {
      "key": "e_1_2_7_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nn900339s"
    },
    {
      "key": "e_1_2_7_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1385/1-59259-823-4:411"
    },
    {
      "key": "e_1_2_7_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/es1034188"
    },
    {
      "key": "e_1_2_7_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.2006.01706.x"
    },
    {
      "key": "e_1_2_7_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jps.10437"
    },
    {
      "key": "e_1_2_7_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bc100010k"
    },
    {
      "key": "e_1_2_7_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.diamond.2004.11.004"
    },
    {
      "key": "e_1_2_7_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.diamond.2005.11.019"
    },
    {
      "key": "e_1_2_7_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(70)90146-6"
    },
    {
      "key": "e_1_2_7_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1103/PhysRevB.82.115449"
    }
  ],
  "container-title": "Small",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fsmll.201101193",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/smll.201101193",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        7
      ]
    ],
    "date-time": "2023-10-07T19:57:09Z",
    "timestamp": 1696708629000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/smll.201101193"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2011,
        9,
        12
      ]
    ]
  },
  "references-count": 48,
  "journal-issue": {
    "issue": "21",
    "published-print": {
      "date-parts": [
        [
          2011,
          11,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/smll.201101193"
  ],
  "URL": "http://dx.doi.org/10.1002/smll.201101193",
  "relation": {},
  "ISSN": [
    "1613-6810",
    "1613-6829"
  ],
  "subject": [],
  "container-title-short": "Small",
  "published": {
    "date-parts": [
      [
        2011,
        9,
        12
      ]
    ]
  }
}