DOI: 10.1002/smll.201002300. Synthesis of Biomolecule‐Modified Mesoporous Silica Nanoparticles for Targeted Hydrophobic Drug Delivery to Cancer Cells

Synthesis of Biomolecule‐Modified Mesoporous Silica Nanoparticles for Targeted Hydrophobic Drug Delivery to Cancer Cells

10.1002/smll.201002300

Crossref journal-article

Wiley

Small (311)

Abstract

AbstractSynthetic methodologies integrating hydrophobic drug delivery and biomolecular targeting with mesoporous silica nanoparticles are described. Transferrin and cyclic‐RGD peptides are covalently attached to the nanoparticles utilizing different techniques and provide selectivity between primary and metastatic cancer cells. The increase in cellular uptake of the targeted particles is examined using fluorescence microscopy and flow cytometry. Transferrin‐modified silica nanoparticles display enhancement in particle uptake by Panc‐1 cancer cells over that of normal HFF cells. The endocytotic pathway for these particles is further investigated through plasmid transfection of the transferrin receptor into the normal HFF cell line, which results in an increase in particle endocytosis as compared to unmodified HFF cells. By designing and attaching a synthetic cyclic‐RGD, selectivity between primary cancer cells (BT‐549) and metastatic cancer cells (MDA‐MB 435) is achieved with enhanced particle uptake by the metastatic cancer cell line. Incorporation of the hydrophobic drug Camptothecin into these two types of biomolecular‐targeted nanoparticles causes an increase in mortality of the targeted cancer cells compared to that caused by both the free drug and nontargeted particles. These results demonstrate successful biomolecular‐targeted hydrophobic drug delivery carriers that selectively target specific cancer cells and result in enhanced drug delivery and cell mortality.

Bibliography

Ferris, D. P., Lu, J., Gothard, C., Yanes, R., Thomas, C. R., Olsen, J., Stoddart, J. F., Tamanoi, F., & Zink, J. I. (2011). Synthesis of BiomoleculeâModified Mesoporous Silica Nanoparticles for Targeted Hydrophobic Drug Delivery to Cancer Cells. Small, 7(13), 1816â1826. Portico.

Authors 9

Daniel P. Ferris (first)
Jie Lu (additional)
Chris Gothard (additional)
Rolando Yanes (additional)
Courtney R. Thomas (additional)
John‐Carl Olsen (additional)
J. Fraser Stoddart (additional)
Fuyuhiko Tamanoi (additional)
Jeffrey I. Zink (additional)

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With this system it is interesting to note that there was a 10‐fold increase in particle uptake by MDA‐MB 435 as compared to MCF‐7 cancer cells but only a 20% increase in cell killing over the passively uptaken phosphonated nanoparticles. This difference may be because of the difference in experimental design as particle uptake does not always directly correlate to its observed cytotoxic effect.

Dates

Type	When
Created	14 years, 3 months ago (May 19, 2011, 4:57 a.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 5:26 a.m.)
Indexed	2 months, 1 week ago (June 16, 2025, 2:24 p.m.)
Issued	14 years, 3 months ago (May 19, 2011)
Published	14 years, 3 months ago (May 19, 2011)
Published Online	14 years, 3 months ago (May 19, 2011)
Published Print	14 years, 1 month ago (July 4, 2011)

Funders 0

None

BibTeX

@article{Ferris_2011, title={Synthesis of Biomolecule‐Modified Mesoporous Silica Nanoparticles for Targeted Hydrophobic Drug Delivery to Cancer Cells}, volume={7}, ISSN={1613-6829}, url={http://dx.doi.org/10.1002/smll.201002300}, DOI={10.1002/smll.201002300}, number={13}, journal={Small}, publisher={Wiley}, author={Ferris, Daniel P. and Lu, Jie and Gothard, Chris and Yanes, Rolando and Thomas, Courtney R. and Olsen, John‐Carl and Stoddart, J. Fraser and Tamanoi, Fuyuhiko and Zink, Jeffrey I.}, year={2011}, month=may, pages={1816–1826} }

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