DOI: 10.1002/smll.200800461. Size‐Dependent Cytotoxicity of Monodisperse Silica Nanoparticles in Human Endothelial Cells

Size‐Dependent Cytotoxicity of Monodisperse Silica Nanoparticles in Human Endothelial Cells

10.1002/smll.200800461

Crossref journal-article

Wiley

Small (311)

Abstract

AbstractThe effect that monodisperse amorphous spherical silica particles of different sizes have on the viability of endothelial cells (EAHY926 cell line) is investigated. The results indicate that exposure to silica nanoparticles causes cytotoxic damage (as indicated by lactate dehydrogenase (LDH) release) and a decrease in cell survival (as determined by the tetrazolium reduction, MTT, assay) in the EAHY926 cell line in a dose‐related manner. Concentrations leading to a 50% reduction in cell viability (TC50) for the smallest particles tested (14‐, 15‐, and 16‐nm diameter) ranging from 33 to 47 µg cm−2 of cell culture differ significantly from values assessed for the bigger nanoparticles: 89 and 254 µg cm−2 (diameter of 19 and 60 nm, respectively). Two fine silica particles with diameters of 104 and 335 nm show very low cytotoxic response compared to nanometer‐sized particles with TC50 values of 1095 and 1087 µg cm−2, respectively. The smaller particles also appear to affect the exposed cells faster with cell death (by necrosis) being observed within just a few hours. The surface area of the tested particles is an important parameter in determining the toxicity of monodisperse amorphous silica nanoparticles.

Bibliography

Napierska, D., Thomassen, L. C. J., Rabolli, V., Lison, D., Gonzalez, L., KirschâVolders, M., Martens, J. A., & Hoet, P. H. (2009). SizeâDependent Cytotoxicity of Monodisperse Silica Nanoparticles in Human Endothelial Cells. Small, 5(7), 846â853. Portico.

Authors 8

Dorota Napierska (first)
Leen C. J. Thomassen (additional)
Virginie Rabolli (additional)
Dominique Lison (additional)
Laetitia Gonzalez (additional)
Micheline Kirsch‐Volders (additional)
Johan A. Martens (additional)
Peter H. Hoet (additional)

References 31 Referenced 489

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{'key': 'e_1_2_6_29_2', 'first-page': '118', 'volume-title': 'Methods of Enzymatic Analyses', 'author': 'Vassault A.', 'year': '1983'} / Methods of Enzymatic Analyses by Vassault A. (1983)
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Dates

Type	When
Created	16 years, 5 months ago (March 13, 2009, 6:25 a.m.)
Deposited	1 year, 11 months ago (Sept. 29, 2023, 3:34 a.m.)
Indexed	6 days, 20 hours ago (Aug. 27, 2025, 11:43 a.m.)
Issued	16 years, 5 months ago (April 1, 2009)
Published	16 years, 5 months ago (April 1, 2009)
Published Online	16 years, 5 months ago (April 1, 2009)
Published Print	16 years, 4 months ago (April 6, 2009)

Funders 0

None

BibTeX

@article{Napierska_2009, title={Size‐Dependent Cytotoxicity of Monodisperse Silica Nanoparticles in Human Endothelial Cells}, volume={5}, ISSN={1613-6829}, url={http://dx.doi.org/10.1002/smll.200800461}, DOI={10.1002/smll.200800461}, number={7}, journal={Small}, publisher={Wiley}, author={Napierska, Dorota and Thomassen, Leen C. J. and Rabolli, Virginie and Lison, Dominique and Gonzalez, Laetitia and Kirsch‐Volders, Micheline and Martens, Johan A. and Hoet, Peter H.}, year={2009}, month=apr, pages={846–853} }

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