DOI: 10.1002/sca.4950010303. Membrane blisters: A fixation artifact a study in fixation for scanning electron microscopy

Membrane blisters: A fixation artifact a study in fixation for scanning electron microscopy

10.1002/sca.4950010303

Crossref journal-article

Wiley

Scanning (311)

Abstract

AbstractIt is been shown by scanning electron microscopy that fixation in glutaraldehyde followed by fixation in osmium tetroxide results in the presence of membrane blisters on the surface of a variety of cells. Fixation in glutaraldehyde alone or osmium tetroxide alone does not result in such extensive artifacts. The blisters, usually 0.2–0.6 μm in diameter, are seen by transmission electron microscopy to be membrane‐bound, virtually empty vesicles. It is concluded that the optimum preservation of the cell surface for scanning electron microscopy is provided by fixation in glutaraldehyde alone.

Bibliography

Shelton, E., & Mowczko, W. E. (1978). Membrane blisters: A fixation artifact a study in fixation for scanning electron microscopy. Scanning, 1(3), 166â173. Portico.

Authors 2

E. Shelton (first)
W. E. Mowczko (additional)

References 22 Referenced 32

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{'key': 'e_1_2_1_4_1', 'first-page': '507', 'volume-title': 'Scanning Electron Microscopy', 'author': 'Boyde A', 'year': '1977'} / Scanning Electron Microscopy by Boyde A (1977)
{'key': 'e_1_2_1_5_1', 'first-page': '265', 'volume-title': 'Scanning Electron Microscopy', 'author': 'Boyde A', 'year': '1972'} / Scanning Electron Microscopy by Boyde A (1972)
{'key': 'e_1_2_1_6_1', 'first-page': '1', 'volume-title': 'Practical Methods in Electron Microscopy', 'author': 'Glauert A M', 'year': '1974'} / Practical Methods in Electron Microscopy by Glauert A M (1974)
10.1083/jcb.64.3.644
HastyD L HayE D:Freeze‐fracture studies of the developing' cell surface. II. Particle‐free membrane blisters on glutaraldehyde fixed corneal fibroblasts are artifacts. J Cell Biol (in press) (1978) (10.1083/jcb.78.3.756)
10.1007/BF01005005
{'key': 'e_1_2_1_10_1', 'first-page': '168a', 'article-title': 'Formaldehyde‐glutaraldehyde fixatives containing trinitrocompounds', 'volume': '39', 'author': 'Ito S', 'year': '1978', 'journal-title': 'J Cell Biol'} / J Cell Biol / Formaldehyde‐glutaraldehyde fixatives containing trinitrocompounds by Ito S (1978)
10.1016/0022-2836(73)90394-X
10.1083/jcb.37.1.117
10.1177/25.3.402414
10.1083/jcb.63.1.197
{'key': 'e_1_2_1_15_1', 'volume-title': 'The preparation of cultured cells and soft tissues for scanning electron microscopy', 'author': 'Porter K R', 'year': '1972'} / The preparation of cultured cells and soft tissues for scanning electron microscopy by Porter K R (1972)
10.1016/0014-4827(67)90278-9
10.1083/jcb.17.1.19
10.1126/science.982044
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{'key': 'e_1_2_1_21_1', 'first-page': '368', 'article-title': 'New ultrastructural characteristics of cells fixed in a glutaraldehyde‐osmium mixture', 'volume': '15', 'author': 'Trump B F', 'year': '1966', 'journal-title': 'Lab Invest'} / Lab Invest / New ultrastructural characteristics of cells fixed in a glutaraldehyde‐osmium mixture by Trump B F (1966)
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{'key': 'e_1_2_1_23_1', 'first-page': '545', 'article-title': 'Cytological studies with the phase microscope. 1. The formation of “blisters” on cells in suspension (potocytosis), with observations on the nature of the cellular membrane', 'volume': '24', 'author': 'Zollinger H V', 'year': '1948', 'journal-title': 'Am J Path'} / Am J Path / Cytological studies with the phase microscope. 1. The formation of “blisters” on cells in suspension (potocytosis), with observations on the nature of the cellular membrane by Zollinger H V (1948)

Dates

Type	When
Created	13 years, 10 months ago (Oct. 5, 2011, 2:21 p.m.)
Deposited	1 year, 9 months ago (Nov. 6, 2023, 5:44 p.m.)
Indexed	1 month ago (July 19, 2025, 11:55 p.m.)
Issued	47 years, 7 months ago (Jan. 1, 1978)
Published	47 years, 7 months ago (Jan. 1, 1978)
Published Online	13 years, 10 months ago (Oct. 5, 2011)
Published Print	47 years, 7 months ago (Jan. 1, 1978)

Funders 0

None

BibTeX

@article{Shelton_1978, title={Membrane blisters: A fixation artifact a study in fixation for scanning electron microscopy}, volume={1}, ISSN={1932-8745}, url={http://dx.doi.org/10.1002/sca.4950010303}, DOI={10.1002/sca.4950010303}, number={3}, journal={Scanning}, publisher={Wiley}, author={Shelton, E. and Mowczko, W. E.}, year={1978}, month=jan, pages={166–173} }

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