DOI: 10.1002/sca.20055. Fluorescence Quenching by Colloidal Heavy Metals Nanoparticles: Implications for Correlative Fluorescence and Electron Microscopy Studies

Fluorescence Quenching by Colloidal Heavy Metals Nanoparticles: Implications for Correlative Fluorescence and Electron Microscopy Studies

10.1002/sca.20055

Crossref journal-article

Wiley

Scanning (311)

Abstract

AbstractLabels for correlative immunolabeling in light (LM) and electron microscopy (EM) employing colloidal metal nanoparticles (gold or palladium) and fluorescent dyes (Alexa Fluor, AF) were investigated. The fluorescence signals from direct conjugates (cAu‐IgG‐AF) and from an indirect label system (cAu‐IgG‐anti IgG‐AF) were studied using scanning spectrofluorometry and fluorescence light microscopy.Direct conjugation of protein—AF, IgG‐AF or FGN‐AF to 18 and 5 nm colloidal gold (cAu18 and cAu5) or 12 nm colloidal palladium particles (cPd12) resulted in nearly completely quenched fluorescence signals (>99 %) at excitation wavelengths of 488, 546 and 594 nm. In contrast, indirect conjugation, when colloidal metal particles and AF were conjugated to primary or secondary antibody, respectively (cAu‐IgG‐antiIgG‐AF), sufficient fluorescence signal was detected.Commercially available conjugates, consisting of IgG‐AF‐cAu5 and IgG‐AF‐cAu10, were also tested and proved to be a mixture of IgG‐AF (unbound to cAu) and cAu‐IgG‐AF. SCANNING 29: 000‐V000, 2007. © 2007 Wiley Periodicals, Inc.

Bibliography

Kandela, I. K., & Albrecht, R. M. (2007). Fluorescence Quenching by Colloidal Heavy Metals Nanoparticles: Implications for Correlative Fluorescence and Electron Microscopy Studies. Scanning, 29(4), 152â161. Portico.

Authors 2

Irawati K. Kandela (first)
Ralph M. Albrecht (additional)

References 25 Referenced 36

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{'issue': '2', 'key': 'e_1_2_1_8_1', 'first-page': '727', 'article-title': 'Correlative light and electron microscopy of platelet adhesion and fibrinogen receptor expression using colloidal‐gold labeling', 'volume': '1', 'author': 'Goodman SL', 'year': '1987', 'journal-title': 'Scanning Microsc'} / Scanning Microsc / Correlative light and electron microscopy of platelet adhesion and fibrinogen receptor expression using colloidal‐gold labeling by Goodman SL (1987)
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{'key': 'e_1_2_1_14_1', 'first-page': '765', 'article-title': 'Utilization of immunogold labeling to compare the adsorption behavior of fibrinogen, fibronectin and albumin on polymers', 'volume': '1', 'author': 'Murthy K', 'year': '1987', 'journal-title': 'Scanning Microsc'} / Scanning Microsc / Utilization of immunogold labeling to compare the adsorption behavior of fibrinogen, fibronectin and albumin on polymers by Murthy K (1987)
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{'key': 'e_1_2_1_17_1', 'first-page': '339', 'article-title': 'Surface characterization of biomaterials by immunogold staining–quantitative analysis', 'volume': '1', 'author': 'Park K', 'year': '1987', 'journal-title': 'Scanning Microsc'} / Scanning Microsc / Surface characterization of biomaterials by immunogold staining–quantitative analysis by Park K (1987)
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Dates

Type	When
Created	18 years, 3 months ago (May 3, 2007, 4:46 p.m.)
Deposited	1 year, 9 months ago (Nov. 15, 2023, 10:47 a.m.)
Indexed	11 months, 1 week ago (Sept. 11, 2024, 4:31 a.m.)
Issued	18 years, 3 months ago (May 3, 2007)
Published	18 years, 3 months ago (May 3, 2007)
Published Online	18 years, 3 months ago (May 3, 2007)
Published Print	18 years, 1 month ago (July 1, 2007)

Funders 0

None

BibTeX

@article{Kandela_2007, title={Fluorescence Quenching by Colloidal Heavy Metals Nanoparticles: Implications for Correlative Fluorescence and Electron Microscopy Studies}, volume={29}, ISSN={1932-8745}, url={http://dx.doi.org/10.1002/sca.20055}, DOI={10.1002/sca.20055}, number={4}, journal={Scanning}, publisher={Wiley}, author={Kandela, Irawati K. and Albrecht, Ralph M.}, year={2007}, month=may, pages={152–161} }

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