DOI: 10.1002/rcm.1290091115. Delayed extraction matrix‐assisted laser desorption time‐of‐flight mass spectrometry

Delayed extraction matrix‐assisted laser desorption time‐of‐flight mass spectrometry

10.1002/rcm.1290091115

Crossref journal-article

Wiley

Rapid Communications in Mass Spectrometry (311)

Abstract

AbstractA new delayed extraction matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight (TOF) mass spectrometer system is described which provides dramatically improved performance over that obtained with identical TOF analyzers operated with constant electrical fields. In this system the ions formed by MALDI are produced in a weak electrical field, and subsequently extracted by application of a high voltage pulse after a predetermined time delay. Three parameters can be independently adjusted to optimize the performance. These include the field magnitude and direction during and immediately following ion production, the magnitude of the extraction field, and the time delay between the laser pulse and application of the extraction field. Theoretical equations are presented which guide the selection of these parameters to optimize the performance.Results are presented from three TOF analyzers of similar design, but ranging in size from a 1.3 m linear analyzer to a 6.4 m reflector. In all cases delayed extraction provides substantially improved resolution over that obtained from static operation. In the smallest linear analyzer resolution of M/ΔM (full width at half maximum) of 4000 is demonstrated for angiotensin I, and with the largest reflecting analyzer, the isotope peaks of bovine insulin are resolved nearly to baseline. With larger proteins, the isotopic peaks are not resolved, but resolution approaching the width of the isotopic envelope is obtained. Resolution improvement is demonstrated for proteins up to 30 kDa. In addition to improved resolution, delayed extraction is shown to improve the quality of MALDI mass spectra by suppressing matrix background, reducing chemical noise, and minimizing the effect of laser intensity on performance.

Bibliography

Vestal, M. L., Juhasz, P., & Martin, S. A. (1995). Delayed extraction matrixâassisted laser desorption timeâofâflight mass spectrometry. Rapid Communications in Mass Spectrometry, 9(11), 1044â1050. Portico.

Authors 3

M. L. Vestal (first)
P. Juhasz (additional)
S. A. Martin (additional)

References 18 Referenced 430

10.1016/0006-291X(74)90285-X
10.1016/0168-1176(87)87041-6
10.1002/rcm.1290020802
10.1016/0076-6879(90)93420-P
10.1021/ac00073a018
10.1021/ac00073a022
{'key': 'e_1_2_1_8_2', 'first-page': '45', 'volume': '37', 'author': 'Mamyrin B. A.', 'year': '1973', 'journal-title': 'Soviet Phys. JETP'} / Soviet Phys. JETP by Mamyrin B. A. (1973)
10.1016/0009-2614(91)90384-L
10.1002/rcm.1290061109
10.1063/1.1715212
W. C.Wiley U. S. Patent 2 685 035.
{'key': 'e_1_2_1_12_2', 'first-page': '63', 'volume-title': "Desorption '94‐Mass Spectrometry of Large Organic Ions by Particle and Photon Induced Desorption, March 27–31, 1994", 'author': 'Brown R. S.'} / Desorption '94‐Mass Spectrometry of Large Organic Ions by Particle and Photon Induced Desorption, March 27–31, 1994 by Brown R. S.
10.1021/ac00109a015
10.1002/rcm.1290081102
10.1021/ac00109a007
{'key': 'e_1_2_1_16_2', 'volume-title': 'These are commercially available analyzers from Perseptive Biosystems'} / These are commercially available analyzers from Perseptive Biosystems
10.1021/ac00099a029
10.1002/rcm.1290060415

Dates

Type	When
Created	20 years, 2 months ago (May 27, 2005, 9:38 p.m.)
Deposited	1 year, 9 months ago (Oct. 26, 2023, 1:42 a.m.)
Indexed	2 weeks ago (Aug. 7, 2025, 4:54 a.m.)
Issued	30 years, 7 months ago (Jan. 1, 1995)
Published	30 years, 7 months ago (Jan. 1, 1995)
Published Online	20 years, 4 months ago (April 8, 2005)
Published Print	30 years, 7 months ago (Jan. 1, 1995)

Funders 0

None

BibTeX

@article{Vestal_1995, title={Delayed extraction matrix‐assisted laser desorption time‐of‐flight mass spectrometry}, volume={9}, ISSN={1097-0231}, url={http://dx.doi.org/10.1002/rcm.1290091115}, DOI={10.1002/rcm.1290091115}, number={11}, journal={Rapid Communications in Mass Spectrometry}, publisher={Wiley}, author={Vestal, M. L. and Juhasz, P. and Martin, S. A.}, year={1995}, month=jan, pages={1044–1050} }

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