DOI: 10.1002/rcm.1290080811. Mass determination of human immunoglobulin IgM using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Mass determination of human immunoglobulin IgM using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

10.1002/rcm.1290080811

Crossref journal-article

Wiley

Rapid Communications in Mass Spectrometry (311)

Abstract

AbstractMonoclonal human immunoglobulin IgM has been analyzed using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. Using a matrix of sinapinic acid with a laser wavelength of 355 nm, positive ions with 2 to 6 charges were observed in the mass spectra. Two major molecular species were present. The molecular weights estimated from the multiply‐charged signals were found to decrease systematically with decrease in the charge state of the ion. This effect was determined to stem from the non‐linear influence of the initial kinetic energy of desorption on the final kinetic energy of the multiply‐charged species. A method of successive approximations, with the molecular weights and desorption velocities of the analytes as variables, was used to correct for these non‐linearities. Mean molecular weights for the two IgM species were found to be 939 000 ± 2000 and 982 000 ± 2000 Da. An initial desorption velocity of 565 ± 10 ms/s was determined for both.

Bibliography

Nelson, R. W., Dogruel, D., Williams, P., & Beavis, R. (1994). Mass determination of human immunoglobulin IgM using matrixâassisted laser desorption/ionization timeâofâflight mass spectrometry. Rapid Communications in Mass Spectrometry, 8(8), 627â631. Portico.

Authors 4

Randall W. Nelson (first)
David Dogruel (additional)
Peter Williams (additional)
Ronald Beavis (additional)

References 9 Referenced 53

10.1021/ac00171a028
10.1002/rcm.1290020802
10.1021/ac00216a020
10.1016/S0003-2670(00)83645-4
10.1073/pnas.87.17.6873
10.1021/ac00081a008
10.1016/0009-2614(91)90384-L
10.1007/978-1-4684-7926-3_28
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Dates

Type	When
Created	20 years, 2 months ago (May 29, 2005, 6:05 a.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 2:19 a.m.)
Indexed	4 months, 3 weeks ago (April 2, 2025, 3:45 a.m.)
Issued	31 years ago (Aug. 1, 1994)
Published	31 years ago (Aug. 1, 1994)
Published Online	20 years, 4 months ago (April 8, 2005)
Published Print	31 years ago (Aug. 1, 1994)

Funders 0

None

BibTeX

@article{Nelson_1994, title={Mass determination of human immunoglobulin IgM using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry}, volume={8}, ISSN={1097-0231}, url={http://dx.doi.org/10.1002/rcm.1290080811}, DOI={10.1002/rcm.1290080811}, number={8}, journal={Rapid Communications in Mass Spectrometry}, publisher={Wiley}, author={Nelson, Randall W. and Dogruel, David and Williams, Peter and Beavis, Ronald}, year={1994}, month=aug, pages={627–631} }

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