DOI: 10.1002/prot.340200303. Conservation and prediction of solvent accessibility in protein families

Conservation and prediction of solvent accessibility in protein families

10.1002/prot.340200303

Crossref journal-article

Wiley

Proteins: Structure, Function, and Bioinformatics (311)

Abstract

AbstractCurrently, the prediction of three‐dimensional (3D) protein structure from sequence alone is an exceedingly difficult task. As an intermediate step, a much simpler task has been pursued extensively: predicting 1D strings of secondary structure. Here, we present an analysis of another 1D projection from 3D structure: the relative solvent accessibility of each residue. We show that solvent accessibility is less conserved in 3D homologues than is secondary structure, and hence is predicted less accurately from automatic homology modeling; the correlation coefficient of relative solvent accessibility between 3D homologues is only 0.77, and the average accuracy of predictions based on sequence alignments is only 0.68. The latter number provides an effective upper limit on the accuracy of predicting accessibility from sequence when homology modeling is not possible. We introduce a neural network system that predicts relative solvent accessibility (projected onto ten discrete states) using evolutionary profiles of amino acid substitutions derived from multiple sequence alignments. Evaluated in a cross‐validation test on 238 unique proteins, the correlation between predicted and observed relative accessibility is 0.54. Interpreted in terms of a three‐state (buried, intermediate, exposed) description of relative accessibility, the fraction of correctly predicted residue states is about 58%. In absolute terms this accuracy appears poor, but given the relatively low conservation of accessibility in 3D families, the network system is not far from its likely optimal performance. The most reliably predicted fraction of the residues (50%) is predicted as accurately as by automatic homology modeling. Prediction is best for buried residues, e.g., 86% of the completely buried sites are correctly predicted as having 0% relative accessibility. © 1994 Wiley‐Liss, Inc.

Bibliography

Rost, B., & Sander, C. (1994). Conservation and prediction of solvent accessibility in protein families. Proteins: Structure, Function, and Bioinformatics, 20(3), 216â226. Portico.

Authors 2

Burkhard Rost (first)
Chris Sander (additional)

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Dates

Type	When
Created	20 years, 2 months ago (May 28, 2005, 9:37 p.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 9:59 p.m.)
Indexed	2 weeks, 1 day ago (Aug. 7, 2025, 4:54 a.m.)
Issued	30 years, 9 months ago (Nov. 1, 1994)
Published	30 years, 9 months ago (Nov. 1, 1994)
Published Online	21 years, 6 months ago (Feb. 3, 2004)
Published Print	30 years, 9 months ago (Nov. 1, 1994)

Funders 0

None

BibTeX

@article{Rost_1994, title={Conservation and prediction of solvent accessibility in protein families}, volume={20}, ISSN={1097-0134}, url={http://dx.doi.org/10.1002/prot.340200303}, DOI={10.1002/prot.340200303}, number={3}, journal={Proteins: Structure, Function, and Bioinformatics}, publisher={Wiley}, author={Rost, Burkhard and Sander, Chris}, year={1994}, month=nov, pages={216–226} }

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