Functional implications of interleukin‐1β based on the three‐dimensional structure
10.1002/prot.340120103
Crossref journal-article
Wiley
Proteins: Structure, Function, and Bioinformatics (311)
Abstract

AbstractThe molecular structure of interleukin‐1β, a hormone‐like cytokine with roles in several disease processes, has been determined at 2.0 Å resolution and refined to a crystallographic R‐factor of 0.19. The frame‐work of this molecule consists of 12 antiparallel β‐strands exhibiting pseudo‐3‐fold symmetry. Six of the strands make up a β‐barrel with polar residues concentrated at either end. Analysis of the three‐dimensional structure, together with results from site‐directed mutagenesis and biochemical and immunological studies, suggest that the core of the β‐barrel plays an important functional role. A large patch of charged residues on one end of the barrel is proposed as the binding surface with which IL‐1 interacts with its receptor.

Bibliography

Veerapandian, B., Gilliland, G. L., Raag, R., Svensson, A. L., Masui, Y., Hirai, Y., & Poulos, T. L. (1992). Functional implications of interleukin‐1β based on the three‐dimensional structure. Proteins: Structure, Function, and Bioinformatics, 12(1), 10–23. Portico.

Authors 7
  1. B. Veerapandian (first)
  2. Gary L. Gilliland (additional)
  3. Reetta Raag (additional)
  4. Anders L. Svensson (additional)
  5. Yoshihiro Masui (additional)
  6. Yoshikatsu Hirai (additional)
  7. Thomas L. Poulos (additional)
References 60 Referenced 64
  1. 10.1093/clinids/6.1.51
  2. 10.1016/0167-5699(86)90124-6
  3. {'key': 'e_1_2_1_4_2', 'first-page': '210', 'volume-title': 'Immunophysiology, Chapter 11, Immunophysiologic Role of Interleukin‐1', 'author': 'Durum K. S.', 'year': '1990'} / Immunophysiology, Chapter 11, Immunophysiologic Role of Interleukin‐1 by Durum K. S. (1990)
  4. 10.1016/S0065-2776(08)60642-2
  5. 10.1159/000157039
  6. 10.1073/pnas.81.24.7907
  7. 10.1038/315641a0
  8. 10.1084/jem.162.3.790
  9. {'key': 'e_1_2_1_10_2', 'first-page': '8473', 'article-title': 'IL1b may be secreted from monocytes as inactive 31Kda precursor and processed later', 'volume': '263', 'author': 'Hazuda D. J.', 'year': '1988', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / IL1b may be secreted from monocytes as inactive 31Kda precursor and processed later by Hazuda D. J. (1988)
  10. {'key': 'e_1_2_1_11_2', 'first-page': '6318', 'article-title': 'IL1b precursor can be processed after secretion by several proteases released at inflammatory sites', 'volume': '265', 'author': 'Hazuda D. J.', 'year': '1990', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / IL1b precursor can be processed after secretion by several proteases released at inflammatory sites by Hazuda D. J. (1990)
  11. {'key': 'e_1_2_1_12_2', 'first-page': '14526', 'article-title': 'Human pro‐IL1‐β is cleaved solely between the residues Asp116‐Ala117 (prosequence numbering)', 'volume': '265', 'author': 'Sleath P. R.', 'year': '1990', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Human pro‐IL1‐β is cleaved solely between the residues Asp116‐Ala117 (prosequence numbering) by Sleath P. R. (1990)
  12. {'key': 'e_1_2_1_13_2', 'first-page': '2941', 'article-title': 'IL1‐beta is active only after processing the precursor', 'volume': '262', 'author': 'Mosley B.', 'year': '1987', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / IL1‐beta is active only after processing the precursor by Mosley B. (1987)
  13. 10.1038/324266a0
  14. 10.1073/pnas.83.4.1060
  15. {'key': 'e_1_2_1_16_2', 'first-page': '4509', 'article-title': 'IL1‐α and IL1‐β bind to the same receptor protein with similar affinities on target cells', 'volume': '136', 'author': 'Kilian P. L.', 'year': '1986', 'journal-title': 'J. Immunol.'} / J. Immunol. / IL1‐α and IL1‐β bind to the same receptor protein with similar affinities on target cells by Kilian P. L. (1986)
  16. {'key': 'e_1_2_1_17_2', 'first-page': '4496', 'article-title': 'IL1‐α and IL1‐β bind to the same receptor protein with similar affinities on target cells', 'volume': '136', 'author': 'Matsushima K.', 'year': '1986', 'journal-title': 'J. Immunol.'} / J. Immunol. / IL1‐α and IL1‐β bind to the same receptor protein with similar affinities on target cells by Matsushima K. (1986)
  17. Giovine F. S. Duff G. W.Overview of the biology of IL‐1.Immunol. Today13–20 1990.
  18. {'key': 'e_1_2_1_19_2', 'first-page': '12323', 'article-title': 'Crystallisation report of IL1‐β', 'volume': '262', 'author': 'Gilliland G. L.', 'year': '1987', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Crystallisation report of IL1‐β by Gilliland G. L. (1987)
  19. 10.1073/pnas.86.24.9667
  20. 10.1016/0022-2836(89)90606-2
  21. 10.1021/bi00223a005
  22. 10.1021/bi00463a009
  23. 10.1107/S0021889887086436
  24. Steigemann W.PROTEIN: A program system for the crystal structure analysis of proteins (Version 85). Ph.D. Thesis Technical University Munich 1974.
  25. 10.1016/0076-6879(85)15009-3
  26. {'key': 'e_1_2_1_27_2', 'first-page': '303', 'volume-title': 'Computational Crystallography', 'author': 'Jones T. A.', 'year': '1982'} / Computational Crystallography by Jones T. A. (1982)
  27. 10.1002/j.1460-2075.1986.tb04287.x
  28. {'key': 'e_1_2_1_29_2', 'first-page': '126', 'volume-title': 'Crystallographic Computing 4: Techniques and New Technologies', 'author': 'Brunger A. T.', 'year': '1988'} / Crystallographic Computing 4: Techniques and New Technologies by Brunger A. T. (1988)
  29. 10.1016/0097-8485(82)85001-8
  30. 10.1107/S0108767385000915
  31. {'key': 'e_1_2_1_32_2', 'first-page': '114', 'volume-title': 'Computing Methods in Crystallography', 'author': 'Cruickshank D. W. J.', 'year': '1965'} / Computing Methods in Crystallography by Cruickshank D. W. J. (1965)
  32. 10.1016/S0022-2836(77)80200-3
  33. 10.1107/S0365110X52002161
  34. 10.1016/S0065-3233(08)60402-7
  35. 10.1016/0022-2836(71)90324-X
  36. {'key': 'e_1_2_1_37_2', 'first-page': '52', 'article-title': 'The crystal structure determination of soybean trypsin inhibitor', 'volume': '249', 'author': 'Blow D. M.', 'year': '1974', 'journal-title': 'Nature (London)'} / Nature (London) / The crystal structure determination of soybean trypsin inhibitor by Blow D. M. (1974)
  37. 10.1021/bi00717a024
  38. 10.1002/bip.360221211 / Biopolymers / Dictionary of protein secondary structure: Pattern recognition of hydrogen‐bonded and geometrical features by Kabsh W. (1983)
  39. 10.1016/S0065-3233(08)60520-3
  40. 10.1126/science.2969618
  41. 10.1016/S0021-9258(18)38002-5 / J. Biol. Chem. / Comparison of the crystallographic structures of antigen‐antibody complexes by Davies D. R. (1988)
  42. 10.1126/science.2333521
  43. {'key': 'e_1_2_1_44_2', 'first-page': '3201', 'article-title': 'Peptide analogues of IL1 and biochemical assay of their binding to its receptors', 'volume': '137', 'author': 'Antoni G.', 'year': '1986', 'journal-title': 'J. Immunol.'} / J. Immunol. / Peptide analogues of IL1 and biochemical assay of their binding to its receptors by Antoni G. (1986)
  44. 10.1016/S0006-291X(87)80107-9
  45. Herzberg O. Moult J.Analysis of the steric strain in the polypeptide backbone of protein molecules: Identification of residues in the active site region with abnormal ϕ and ψ using well defined high resolution structures. Proteins1991 in press. (10.1002/prot.340110307)
  46. 10.1016/0014-5793(86)81130-9
  47. 10.1111/j.1432-1033.1986.tb10121.x
  48. 10.1016/0014-5793(87)80307-1
  49. {'key': 'e_1_2_1_50_2', 'first-page': '16275', 'article-title': 'Site‐directed mutations in the amino‐terminal region of IL1‐β and biochemical and kinetic studies of its binding to IL1‐receptors', 'volume': '262', 'author': 'Horuk R.', 'year': '1987', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Site‐directed mutations in the amino‐terminal region of IL1‐β and biochemical and kinetic studies of its binding to IL1‐receptors by Horuk R. (1987)
  50. 10.1016/0006-291X(88)90743-7
  51. 10.1111/j.1432-1033.1989.tb14584.x
  52. 10.1016/S0021-9258(19)39265-8 / J. Biol. Chem. / Point mutations at R12G and analysis of its biological activity by equilibrium receptor binding analysis by Gehrke L. (1990)
  53. Veerapandian B. Gilliland L. G. Poulos T. L.Crystallographic structure solution of interleukin‐1 β mutants. Manuscript in preparation.
  54. {'key': 'e_1_2_1_55_2', 'first-page': '800', 'article-title': 'Peptide analogues of IL1 and their immunostimulatory activity', 'volume': '139', 'author': 'Nencioni L.', 'year': '1987', 'journal-title': 'J. Immunol.'} / J. Immunol. / Peptide analogues of IL1 and their immunostimulatory activity by Nencioni L. (1987)
  55. 10.1073/pnas.83.21.8303
  56. 10.1073/pnas.84.13.4572
  57. {'key': 'e_1_2_1_58_2', 'first-page': '2941', 'article-title': 'Peptide analogues of IL1 and their IL1 activity', 'volume': '262', 'author': 'Mosley B.', 'year': '1987', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Peptide analogues of IL1 and their IL1 activity by Mosley B. (1987)
  58. {'key': 'e_1_2_1_59_2', 'first-page': '1975', 'article-title': 'Peptide analogues of IL1 and biological activity', 'volume': '141', 'author': 'Lillquist J. S.', 'year': '1988', 'journal-title': 'J. Immunol.'} / J. Immunol. / Peptide analogues of IL1 and biological activity by Lillquist J. S. (1988)
  59. {'key': 'e_1_2_1_60_2', 'first-page': '16372', 'article-title': 'Peptide analogues of IL1 and their biological activity', 'volume': '263', 'author': 'Jobling S. A.', 'year': '1988', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Peptide analogues of IL1 and their biological activity by Jobling S. A. (1988)
  60. 10.1073/pnas.83.14.5243
Dates
Type When
Created 20 years, 3 months ago (May 28, 2005, 9:15 p.m.)
Deposited 1 year, 10 months ago (Oct. 23, 2023, 7:13 a.m.)
Indexed 1 month ago (Aug. 2, 2025, 1:14 a.m.)
Issued 33 years, 8 months ago (Jan. 1, 1992)
Published 33 years, 8 months ago (Jan. 1, 1992)
Published Online 21 years, 7 months ago (Feb. 3, 2004)
Published Print 33 years, 8 months ago (Jan. 1, 1992)
Funders 0

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@article{Veerapandian_1992, title={Functional implications of interleukin‐1β based on the three‐dimensional structure}, volume={12}, ISSN={1097-0134}, url={http://dx.doi.org/10.1002/prot.340120103}, DOI={10.1002/prot.340120103}, number={1}, journal={Proteins: Structure, Function, and Bioinformatics}, publisher={Wiley}, author={Veerapandian, B. and Gilliland, Gary L. and Raag, Reetta and Svensson, Anders L. and Masui, Yoshihiro and Hirai, Yoshikatsu and Poulos, Thomas L.}, year={1992}, month=jan, pages={10–23} }