DOI: 10.1002/prot.340020404. Electrostatic calculations and model‐building suggest that DNA bound to CAP is sharply bent

Electrostatic calculations and model‐building suggest that DNA bound to CAP is sharply bent

10.1002/prot.340020404

Crossref journal-article

Wiley

Proteins: Structure, Function, and Bioinformatics (311)

Abstract

AbstractTwo observations suggest that DNA, upon binding to E. coli catabolite gene activator protein (CAP), is sharply bent by a total angle of at least 100–150 degrees: (1) The electrostatic protential field of CAP shows regions of positive potential that from a ramp on 3 sides of the protein. (2) The DNA binding site size as determined by DNA ethylation interference with binding, (Majors: “Control of the E. coli Lac Operon at the Molecular Level.” Ph.D. Thesis, Harvard University, Cambridge, 1977) and by relative affinities of DNA fragments of various lengths (Liu‐Johnson et al.: Cell 47:995–1005, 1986) requires severe bending of the DNA to maintain its favorable electrostatic contact with the protein.

Bibliography

Warwicker, J., Engelman, B. P., & Steitz, T. A. (1987). Electrostatic calculations and modelâbuilding suggest that DNA bound to CAP is sharply bent. Proteins: Structure, Function, and Bioinformatics, 2(4), 283â289. Portico.

Authors 3

J. Warwicker (first)
B.P. Engelman (additional)
T.A. Steitz (additional)

References 27 Referenced 53

{'key': 'e_1_2_1_2_2', 'volume-title': 'Biological Macromolecules and Assemblies: Vol. 2‐Nucleic Acids and Interactive Protein', 'author': 'Steitz T.A.', 'year': '1985'} / Biological Macromolecules and Assemblies: Vol. 2‐Nucleic Acids and Interactive Protein by Steitz T.A. (1985)
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10.1016/S0021-9258(18)34101-2 / J. Biol. Chem. / Structure of catabolite gene activator protein at 2.9 Å resolution: Incorporation of amino acid sequence and interaction with cAMP by McKay D.B. (1982)
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{'key': 'e_1_2_1_12_2', 'first-page': '426', 'article-title': 'Catabolite gene activator protein: Structure, homology with other proteins, and cyclic AMP and DNA binding', 'volume': '419', 'author': 'Steitz T.A.', 'year': '1983', 'journal-title': 'Cold Spring Harbor Symp. Quant. Biol. XLVII'} / Cold Spring Harbor Symp. Quant. Biol. XLVII / Catabolite gene activator protein: Structure, homology with other proteins, and cyclic AMP and DNA binding by Steitz T.A. (1983)
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{'key': 'e_1_2_1_27_2', 'first-page': '164', 'volume-title': 'Physical Chemistry of Nucleic Acids', 'author': 'Bloomfield V.A.', 'year': '1974'} / Physical Chemistry of Nucleic Acids by Bloomfield V.A. (1974)
10.1016/0092-8674(80)90613-3

Dates

Type	When
Created	20 years, 3 months ago (May 27, 2005, 7:21 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 10:47 p.m.)
Indexed	1 year, 6 months ago (March 4, 2024, 4 p.m.)
Issued	38 years, 8 months ago (Jan. 1, 1987)
Published	38 years, 8 months ago (Jan. 1, 1987)
Published Online	21 years, 7 months ago (Feb. 3, 2004)
Published Print	38 years, 8 months ago (Jan. 1, 1987)

Funders 0

None

BibTeX

@article{Warwicker_1987, title={Electrostatic calculations and model‐building suggest that DNA bound to CAP is sharply bent}, volume={2}, ISSN={1097-0134}, url={http://dx.doi.org/10.1002/prot.340020404}, DOI={10.1002/prot.340020404}, number={4}, journal={Proteins: Structure, Function, and Bioinformatics}, publisher={Wiley}, author={Warwicker, J. and Engelman, B.P. and Steitz, T.A.}, year={1987}, month=jan, pages={283–289} }

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