DOI: 10.1002/prot.22852. Universality of protein reentrant condensation in solution induced by multivalent metal ions

Universality of protein reentrant condensation in solution induced by multivalent metal ions

10.1002/prot.22852

Crossref journal-article

Wiley

Proteins: Structure, Function, and Bioinformatics (311)

Abstract

AbstractThe effective interactions and phase behavior of protein solutions under strong electrostatic coupling conditions are difficult to understand due to the complex charge pattern and irregular geometry of protein surfaces. This distinguishes them from related systems such as DNA or conventional colloids. In this work, we discuss the question of universality of the reentrant condensation (RC) of proteins in solution induced by multivalent counterions, i.e., redissolution on adding further salts after phase separation, as recently discovered (Zhang et al., Phys Rev Lett 2008; 101:148101). The discussion is based on a systematic investigation of five different proteins with different charge patterns and five different multivalent counterions. Zeta potential measurements confirm the effective charge inversion of proteins in the reentrant regime via binding of multivalent counterions, which is supported by Monte Carlo simulations. Charge inversion by trivalent cations requires an overall negative net charge of the protein. Statistical analysis of a representative set of protein sequences reveals that, in theory, this effect could be possible for about half of all proteins. Our results can be exploited for the control of the phase behavior of proteins, in particular facilitating protein crystallization. Proteins 2010. © 2010 Wiley‐Liss, Inc.

Bibliography

Zhang, F., Weggler, S., Ziller, M. J., Ianeselli, L., Heck, B. S., Hildebrandt, A., Kohlbacher, O., Skoda, M. W. A., Jacobs, R. M. J., & Schreiber, F. (2010). Universality of protein reentrant condensation in solution induced by multivalent metal ions. Proteins: Structure, Function, and Bioinformatics, 78(16), 3450â3457. Portico.

Authors 10

Fajun Zhang (first)
Sophie Weggler (additional)
Michael J. Ziller (additional)
Luca Ianeselli (additional)
Benjamin S. Heck (additional)
Andreas Hildebrandt (additional)
Oliver Kohlbacher (additional)
Maximilian W. A. Skoda (additional)
Robert M. J. Jacobs (additional)
Frank Schreiber (additional)

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{'key': 'e_1_2_6_36_2', 'article-title': 'Monte Carlo simulation of multivalent metal ion‐protein binding', 'author': 'Weggler S', 'journal-title': 'J Phys Chem B'} / J Phys Chem B / Monte Carlo simulation of multivalent metal ion‐protein binding by Weggler S
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Dates

Type	When
Created	15 years ago (Aug. 21, 2010, 11:23 a.m.)
Deposited	1 year, 10 months ago (Oct. 9, 2023, 5:23 p.m.)
Indexed	4 months, 1 week ago (April 16, 2025, 3:47 a.m.)
Issued	14 years, 11 months ago (Sept. 24, 2010)
Published	14 years, 11 months ago (Sept. 24, 2010)
Published Online	14 years, 11 months ago (Sept. 24, 2010)
Published Print	14 years, 8 months ago (Dec. 1, 2010)

Funders 0

None

BibTeX

@article{Zhang_2010, title={Universality of protein reentrant condensation in solution induced by multivalent metal ions}, volume={78}, ISSN={1097-0134}, url={http://dx.doi.org/10.1002/prot.22852}, DOI={10.1002/prot.22852}, number={16}, journal={Proteins: Structure, Function, and Bioinformatics}, publisher={Wiley}, author={Zhang, Fajun and Weggler, Sophie and Ziller, Michael J. and Ianeselli, Luca and Heck, Benjamin S. and Hildebrandt, Andreas and Kohlbacher, Oliver and Skoda, Maximilian W. A. and Jacobs, Robert M. J. and Schreiber, Frank}, year={2010}, month=sep, pages={3450–3457} }

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