DOI: 10.1002/prot.21969. Direct insight into insulin aggregation by 2D NMR complemented by PFGSE NMR

Direct insight into insulin aggregation by 2D NMR complemented by PFGSE NMR

10.1002/prot.21969

Crossref journal-article

Wiley

Proteins: Structure, Function, and Bioinformatics (311)

Abstract

AbstractThe aggregation of Zn(II)‐bound and zinc‐free human insulin was studied in solution using the Hβ∼CH3 crosspeaks of threonine residues in 2D COSY, TOCSY, and NOESY NMR spectra which allow viewing of the oligomers in equilibrium. This is complemented by PFGSE measurements of the translational diffusion coefficient, Di, used for monitoring the changes in equilibrium composition of aggregates on dilution of both insulins in physiological medium. The back calculation of the dilution isotherm allows establishing the association constants for oligomeric equilibria in solution and discussion of the models of association. Proteins 2008. © 2008 Wiley‐Liss, Inc.

Bibliography

Bocian, W., Sitkowski, J., Tarnowska, A., Bednarek, E., KawÈ©cki, R., KoÅºmiÅski, W., & Kozerski, L. (2008). Direct insight into insulin aggregation by 2D NMR complemented by PFGSE NMR. Proteins: Structure, Function, and Bioinformatics, 71(3), 1057â1065. Portico.

Authors 7

Wojciech Bocian (first)
Jerzy Sitkowski (additional)
Anna Tarnowska (additional)
Elżbieta Bednarek (additional)
Robert Kawȩcki (additional)
Wiktor Koźmiński (additional)
Lech Kozerski (additional)

References 46 Referenced 21

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10.1016/S0021-9258(19)50374-X / J Biol Chem / Studies of the association and conformational properties of metal‐free insulin in alkaline sodium chloride solutions by one‐ and two‐dimensional 1H NMR by Kadima W (1992)
10.1016/S0021-9258(19)39381-0 / J Biol Chem / 1H NMR spectrum of the native human insulin monomer. Evidence for conformational differences between the monomer and aggregated forms by Roy M (1990)
{'key': 'e_1_2_6_8_2', 'series-title': 'Encyclopedia of nuclear magnetic resonance', 'first-page': '364', 'author': 'Price WS', 'year': '2002'} / Encyclopedia of nuclear magnetic resonance by Price WS (2002)
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Dates

Type	When
Created	16 years, 6 months ago (Feb. 27, 2009, 8:53 a.m.)
Deposited	1 year, 10 months ago (Oct. 15, 2023, 8:29 a.m.)
Indexed	2 months, 2 weeks ago (June 18, 2025, 2:10 a.m.)
Issued	17 years, 6 months ago (Feb. 7, 2008)
Published	17 years, 6 months ago (Feb. 7, 2008)
Published Online	17 years, 6 months ago (Feb. 7, 2008)
Published Print	17 years, 3 months ago (May 15, 2008)

Funders 0

None

BibTeX

@article{Bocian_2008, title={Direct insight into insulin aggregation by 2D NMR complemented by PFGSE NMR}, volume={71}, ISSN={1097-0134}, url={http://dx.doi.org/10.1002/prot.21969}, DOI={10.1002/prot.21969}, number={3}, journal={Proteins: Structure, Function, and Bioinformatics}, publisher={Wiley}, author={Bocian, Wojciech and Sitkowski, Jerzy and Tarnowska, Anna and Bednarek, Elżbieta and Kawȩcki, Robert and Koźmiński, Wiktor and Kozerski, Lech}, year={2008}, month=feb, pages={1057–1065} }

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