DOI: 10.1002/prot.21544. Role of the individual domains of translation termination factor eRF1 in GTP binding to eRF3

Role of the individual domains of translation termination factor eRF1 in GTP binding to eRF3

10.1002/prot.21544

Crossref journal-article

Wiley

Proteins: Structure, Function, and Bioinformatics (311)

Abstract

AbstractEukaryotic translational termination is triggered by polypeptide release factors eRF1, eRF3, and one of the three stop codons at the ribosomal A‐site. Isothermal titration calorimetry shows that (i) the separated MC, M, and C domains of human eRF1 bind to eRF3; (ii) GTP binding to eRF3 requires complex formation with either the MC or M + C domains; (iii) the M domain interacts with the N and C domains; (iv) the MC domain and Mg2+ induce GTPase activity of eRF3 in the ribosome. We suggest that GDP binding site of eRF3 acquires an ability to bind γ‐phosphate of GTP if altered by cooperative action of the M and C domains of eRF1. Thus, the stop‐codon decoding is associated with the N domain of eRF1 while the GTPase activity of eRF3 is controlled by the MC domain of eRF1 demonstrating a substantial structural uncoupling of these two activities though functionally they are interrelated. Proteins 2008. © 2007 Wiley‐Liss, Inc.

Bibliography

Kononenko, A. V., Mitkevich, V. A., Dubovaya, V. I., Kolosov, P. M., Makarov, A. A., & Kisselev, L. L. (2007). Role of the individual domains of translation termination factor eRF1 in GTP binding to eRF3. Proteins: Structure, Function, and Bioinformatics, 70(2), 388â393. Portico.

Authors 6

Artem V. Kononenko (first)
Vladimir A. Mitkevich (additional)
Vera I. Dubovaya (additional)
Peter M. Kolosov (additional)
Alexander A. Makarov (additional)
Lev L. Kisselev (additional)

References 25 Referenced 33

10.1093/emboj/cdg017
10.1016/S0079-6603(03)01011-0
10.1016/j.cell.2006.04.035
{'key': 'e_1_2_6_5_2', 'first-page': '334', 'article-title': 'Eukaryotic polypeptide chain release factor eRF3 is an eRF1‐ and ribosome‐dependent guanosine triphosphatase', 'volume': '2', 'author': 'Frolova L', 'year': '1996', 'journal-title': 'RNA'} / RNA / Eukaryotic polypeptide chain release factor eRF3 is an eRF1‐ and ribosome‐dependent guanosine triphosphatase by Frolova L (1996)
10.1016/S0092-8674(00)80667-4
10.1093/nar/gki927
10.1017/S135583829999043X
10.1093/nar/29.19.3982
10.1016/S1097-2765(02)00691-3
10.1038/nature01225
10.1046/j.1365-2958.2003.03301.x
10.1038/nature01224
10.1016/j.cell.2005.09.039
10.1017/S135583829998216X
10.1016/S0014-5793(98)01669-X
10.1016/j.biochi.2006.06.001
10.1093/nar/gkl549
{'key': 'e_1_2_6_19_2', 'first-page': '37', 'volume-title': 'Biocalorimetry 2: applications calorimetry in the biological sciences', 'author': 'Thomson JA', 'year': '2004'} / Biocalorimetry 2: applications calorimetry in the biological sciences by Thomson JA (2004)
{'key': 'e_1_2_6_20_2', 'first-page': '310', 'article-title': 'Influence of individual domains of the translation termination factor eRF1 on induction of the GTPase activity of the translation termination factor eRF3', 'volume': '40', 'author': 'Dubovaya VI', 'year': '2006', 'journal-title': 'Mol Biol (Mosc)'} / Mol Biol (Mosc) / Influence of individual domains of the translation termination factor eRF1 on induction of the GTPase activity of the translation termination factor eRF3 by Dubovaya VI (2006)
10.1074/jbc.M407252200
{'key': 'e_1_2_6_22_2', 'first-page': '103', 'article-title': 'Molecular modeling of structure of eukaryotic ribosomal translation termination complex', 'volume': '41', 'author': 'Vorobiev DG', 'year': '2007', 'journal-title': 'Mol Biol (Mosc)'} / Mol Biol (Mosc) / Molecular modeling of structure of eukaryotic ribosomal translation termination complex by Vorobiev DG (2007)
{'key': 'e_1_2_6_23_2', 'first-page': '100', 'article-title': 'Thermal denaturation of eukaryotic class‐1 translation termination factor eRF1. Relationship between stability of the eRF1 molecule and alteration of functional activity of its mutants', 'volume': '40', 'author': 'Mitkevich VA', 'year': '2006', 'journal-title': 'Mol Biol (Mosc)'} / Mol Biol (Mosc) / Thermal denaturation of eukaryotic class‐1 translation termination factor eRF1. Relationship between stability of the eRF1 molecule and alteration of functional activity of its mutants by Mitkevich VA (2006)
10.1016/S0092-8674(01)00508-6
10.1016/S0022-2836(03)00813-1
10.1016/S1097-2765(04)00206-0

Dates

Type	When
Created	18 years, 1 month ago (Aug. 6, 2007, 5:46 p.m.)
Deposited	1 year, 10 months ago (Oct. 10, 2023, 1:38 a.m.)
Indexed	3 weeks, 4 days ago (Aug. 12, 2025, 5:29 p.m.)
Issued	18 years, 1 month ago (Aug. 6, 2007)
Published	18 years, 1 month ago (Aug. 6, 2007)
Published Online	18 years, 1 month ago (Aug. 6, 2007)
Published Print	17 years, 7 months ago (Feb. 1, 2008)

Funders 0

None

BibTeX

@article{Kononenko_2007, title={Role of the individual domains of translation termination factor eRF1 in GTP binding to eRF3}, volume={70}, ISSN={1097-0134}, url={http://dx.doi.org/10.1002/prot.21544}, DOI={10.1002/prot.21544}, number={2}, journal={Proteins: Structure, Function, and Bioinformatics}, publisher={Wiley}, author={Kononenko, Artem V. and Mitkevich, Vladimir A. and Dubovaya, Vera I. and Kolosov, Peter M. and Makarov, Alexander A. and Kisselev, Lev L.}, year={2007}, month=aug, pages={388–393} }

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