DOI: 10.1002/pro.584. The structure of human ubiquitin in 2‐methyl‐2,4‐pentanediol: A new conformational switch

The structure of human ubiquitin in 2‐methyl‐2,4‐pentanediol: A new conformational switch

10.1002/pro.584

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractA new crystal structure of human ubiquitin is reported at 1.8 Å resolution. Compared with the other known crystal structure or the solution NMR structure of monomeric human ubiquitin, this new structure is similar in its overall fold but differs with respect to the conformation of the backbone in a surface‐exposed region. The conformation reported here resembles conformations previously seen in complex with deubiquinating enzymes, wherein the Asp52/Gly53 main chain and Glu24 side chain move. This movement exposes the backbone carbonyl of Asp52 to the exterior of the molecule, making it possible to engage in hydrogen‐bond contacts with neighboring molecules, rather than in an internal hydrogen bond with the backbone of Glu24. This particular crystal form of ubiquitin has been used in a large number of solid state NMR studies. The structure described here elucidates the origin of many of the chemical shift differences comparing solution and solid state studies.

Bibliography

Huang, K. Y., Amodeo, G. A., Tong, L., & McDermott, A. (2011). The structure of human ubiquitin in 2âmethylâ2,4âpentanediol: A new conformational switch. Protein Science, 20(3), 630â639. Portico.

Authors 4

Kuo Ying Huang (first)
Gabriele A. Amodeo (additional)
Liang Tong (additional)
Ann McDermott (additional)

References 45 Referenced 47

{'key': 'e_1_2_7_2_2', 'volume-title': 'Protein NMR spectroscopy : principles and practice', 'author': 'Cavanagh J', 'year': '2007'} / Protein NMR spectroscopy : principles and practice by Cavanagh J (2007)
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Dates

Type	When
Created	14 years, 7 months ago (Jan. 6, 2011, 5:04 p.m.)
Deposited	1 year, 11 months ago (Sept. 14, 2023, 9:49 p.m.)
Indexed	1 day, 4 hours ago (Aug. 23, 2025, 9:21 p.m.)
Issued	14 years, 6 months ago (Feb. 24, 2011)
Published	14 years, 6 months ago (Feb. 24, 2011)
Published Online	14 years, 6 months ago (Feb. 24, 2011)
Published Print	14 years, 5 months ago (March 1, 2011)

Funders 1

NSF
Awards1
1. MCB 0316248

BibTeX

@article{Huang_2011, title={The structure of human ubiquitin in 2‐methyl‐2,4‐pentanediol: A new conformational switch}, volume={20}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.584}, DOI={10.1002/pro.584}, number={3}, journal={Protein Science}, publisher={Wiley}, author={Huang, Kuo Ying and Amodeo, Gabriele A. and Tong, Liang and McDermott, Ann}, year={2011}, month=feb, pages={630–639} }

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