DOI: 10.1002/pro.5560070720. The interaction of eIF4E with 4E‐BP1 is an induced fit to a completely disordered protein

The interaction of eIF4E with 4E‐BP1 is an induced fit to a completely disordered protein

10.1002/pro.5560070720

Crossref journal-article

Wiley

Protein Science (311)

Abstract

Abstract4E binding protein 1 (4E‐BP1) inhibits translation by binding to the initiation factor eIF4E and is mostly or completely unstructured in both free and bound states. We wished to determine whether the free protein has local structure that could be involved in eIF4E binding. Assignments were obtained using double and triple resonance NMR methods. Residues 4–10, 43–46, and 56–65 could not be assigned, primarily because of a high degree of 1H and 15N chemical shift overlap. Steady‐state {1H}−15N NOEs were measured for 45 residues in the assigned regions. Except for the two C‐terminal residues, the NOEs were between −0.77 and −1.14, indicating a high level of flexibility. Furthermore, the {1H}−15N NOE spectrum recorded with presaturation contained no strong positive signals, making it likely that no other residues have positive or smaller negative NOEs. This implies that 4E‐BPI has no regions of local order in the absence of eIF4E. The interaction therefore appears to be an induced fit to a completely disordered protein molecule.

Bibliography

Fletcher, C. M., & Wagner, G. (1998). The interaction of eIF4E with 4EâBP1 is an induced fit to a completely disordered protein. Protein Science, 7(7), 1639â1642. Portico.

Authors 2

C. Mark Fletcher (first)
Gerhard Wagner (additional)

References 18 Referenced 64

10.1007/BF00417486
10.1002/pro.5560050210
10.1038/nsb0497-285
10.1021/bi00185a040
10.1021/bi972494r
10.1006/jmra.1994.1145
10.1128/MCB.15.9.4990
10.1006/jmrb.1996.0018
10.1038/371762a0
10.1006/jmbi.1997.1369
10.1038/386657a0
{'key': 'e_1_2_1_13_1', 'first-page': '2415', 'article-title': 'The eIF4E‐binding proteins 1 and 2 are negative regulators of cell growth', 'volume': '13', 'author': 'Rousseau D', 'year': '1996', 'journal-title': 'Oncogene'} / Oncogene / The eIF4E‐binding proteins 1 and 2 are negative regulators of cell growth by Rousseau D (1996)
{'key': 'e_1_2_1_14_1', 'first-page': '245', 'volume-title': 'Translutional control', 'author': 'Sonenberg N.', 'year': '1996'} / Translutional control by Sonenberg N. (1996)
10.1006/jmrb.1996.0132
10.1016/S0955-0674(97)80078-6
10.1126/science.277.5330.1310
10.1007/BF00227471
10.1007/BF00398413

Dates

Type	When
Created	16 years, 6 months ago (Feb. 10, 2009, 1:32 a.m.)
Deposited	1 year, 10 months ago (Oct. 28, 2023, 8:26 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 7, 2025, 4:58 p.m.)
Issued	27 years, 2 months ago (July 1, 1998)
Published	27 years, 2 months ago (July 1, 1998)
Published Online	16 years, 8 months ago (Dec. 31, 2008)
Published Print	27 years, 2 months ago (July 1, 1998)

Funders 0

None

BibTeX

@article{Fletcher_1998, title={The interaction of eIF4E with 4E‐BP1 is an induced fit to a completely disordered protein}, volume={7}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560070720}, DOI={10.1002/pro.5560070720}, number={7}, journal={Protein Science}, publisher={Wiley}, author={Fletcher, C. Mark and Wagner, Gerhard}, year={1998}, month=jul, pages={1639–1642} }

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