DOI: 10.1002/pro.5560070715. Crystallization of a trimeric human T cell leukemia virus type 1 gp21 ectodomain fragment as a chimera with maltose‐binding protein

Crystallization of a trimeric human T cell leukemia virus type 1 gp21 ectodomain fragment as a chimera with maltose‐binding protein

10.1002/pro.5560070715

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractWe present a novel protein crystallization strategy, applied to the crystallization of human T cell leukemia virus type I (HTLV‐1) transmembrane protein gp21 lacking the fusion peptide and the transmembrane domain, as a chimera with the Escherichia coli maltose binding protein (MBP). Crystals could not be obtained with a MBP/gp21 fusion protein in which fusion partners were separated by a flexible linker, but were obtained after connecting the MBP C‐terminal α‐helix to the predicted N‐terminal α‐helical sequence of gp21 via three alanine residues. The gp21 sequences conferred a trimeric structure to the soluble fusion proteins as assessed by sedimentation equilibrium and X‐ray diffraction, consistent with the trimeric structures of other retroviral transmembrane proteins. The envelope protein precursor, gp62, is likewise trimeric when expressed in mammalian cells. Our results suggest that MBP may have a general application for the crystallization of proteins containing N‐terminal α‐helical sequences.

Bibliography

Center, R. J., Kobe, B., Wilson, K. A., Teh, T., Kemp, B. E., Poumbourios, P., & Howlett, G. J. (1998). Crystallization of a trimeric human T cell leukemia virus type 1 gp21 ectodomain fragment as a chimera with maltoseâbinding protein. Protein Science, 7(7), 1612â1619. Portico.

Authors 7

Rob J. Center (first)
Bostjan Kobe (additional)
Kirilee A. Wilson (additional)
Trazel Teh (additional)
Bruce E. Kemp (additional)
Pantelis Poumbourios (additional)
Geoffrey J. Howlett (additional)

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Dates

Type	When
Created	16 years, 6 months ago (Feb. 10, 2009, 1:32 a.m.)
Deposited	1 year, 9 months ago (Oct. 28, 2023, 8:24 p.m.)
Indexed	1 month ago (July 20, 2025, 6:47 p.m.)
Issued	27 years, 1 month ago (July 1, 1998)
Published	27 years, 1 month ago (July 1, 1998)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	27 years, 1 month ago (July 1, 1998)

Funders 0

None

BibTeX

@article{Center_1998, title={Crystallization of a trimeric human T cell leukemia virus type 1 gp21 ectodomain fragment as a chimera with maltose‐binding protein}, volume={7}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560070715}, DOI={10.1002/pro.5560070715}, number={7}, journal={Protein Science}, publisher={Wiley}, author={Center, Rob J. and Kobe, Bostjan and Wilson, Kirilee A. and Teh, Trazel and Kemp, Bruce E. and Poumbourios, Pantelis and Howlett, Geoffrey J.}, year={1998}, month=jul, pages={1612–1619} }

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