DOI: 10.1002/pro.5560070315. Spider minor ampullate silk proteins contain new repetitive sequences and highly conserved non‐silk‐like “spacer regions”

Spider minor ampullate silk proteins contain new repetitive sequences and highly conserved non‐silk‐like “spacer regions”

10.1002/pro.5560070315

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractSpider minor ampullate silk is a strong non‐elastic deformably stretchable silk used in web formation. This silk from Nephila clavipes is composed of two proteins, MiSp 1 and 2, whose transcripts are 9.5 and 7.5 kb, respectively, as determined by northern blots. Both MiSp proteins are organized into a predominantly repetitive region and a small nonrepetitive carboxy terminal region. These highly repetitive regions are composed mainly of glycine and alanine, but also contain tyrosine, glutamine, and arginine. The sequences are mainly GGX and GA repeats. The repetitive regions are interrupted by nonrepetitive serine‐rich spacer regions. Although the sequences of the spacer regions differ from the repetitive regions, sequences of the spacers from different regions of the proteins are nearly identical. The sequence differences between major and minor ampullate silks may explain the differing mechanical properties of the fibers.

Bibliography

Colgin, M. A., & Lewis, R. V. (1998). Spider minor ampullate silk proteins contain new repetitive sequences and highly conserved nonâsilkâlike âspacer regions.â Protein Science, 7(3), 667â672. Portico.

Authors 2

Mark A. Colgin (first)
Randolph V. Lewis (additional)

References 26 Referenced 146

10.1016/S0021-9258(17)37425-2
{'key': 'e_1_2_1_3_1', 'first-page': '545', 'article-title': 'X‐ray studies of poly‐L‐alanine', 'volume': '52', 'author': 'Brown L', 'year': '1960', 'journal-title': 'Faraday Soc Trans'} / Faraday Soc Trans / X‐ray studies of poly‐L‐alanine by Brown L (1960)
DongZ.1992. [Doctoral dissertation]. University of Wyoming pp50–52.
10.1016/0003-9861(91)90262-H
10.1002/bip.360311309
{'key': 'e_1_2_1_7_1', 'first-page': '155', 'volume-title': 'Silk polymers', 'author': 'Gillespie DB', 'year': '1994'} / Silk polymers by Gillespie DB (1994)
10.1016/0160-9327(86)90049-9
10.1126/science.272.5258.112
10.1006/jmbi.1997.1478
10.1016/0076-6879(87)52018-3
10.1016/S0021-9258(18)41777-2
{'key': 'e_1_2_1_13_1', 'first-page': '222', 'volume-title': 'Silk polymers', 'author': 'Hinman MB', 'year': '1994'} / Silk polymers by Hinman MB (1994)
10.1021/ar00021a002
{'key': 'e_1_2_1_15_1', 'first-page': '20', 'article-title': 'Spiders and their silk', 'volume': '25', 'author': 'Lucas F.', 'year': '1986', 'journal-title': 'Discovery'} / Discovery / Spiders and their silk by Lucas F. (1986)
10.1006/abio.1994.1392
10.1007/BF00175878
10.1002/(SICI)1099-1352(199701/02)10:1<1::AID-JMR338>3.0.CO;2-7
{'volume-title': 'Molecular cloning: A laboratory manual', 'year': '1989', 'author': 'Sambrook J', 'key': 'e_1_2_1_19_1'} / Molecular cloning: A laboratory manual by Sambrook J (1989)
10.1126/science.271.5245.84
{'key': 'e_1_2_1_21_1', 'first-page': '5', 'article-title': 'Comparison of physical properties of three silks from Nephila clavipes and Araneus gemmoides', 'volume': '22', 'author': 'Stauffer SL', 'year': '1994', 'journal-title': 'J Arachnol'} / J Arachnol / Comparison of physical properties of three silks from Nephila clavipes and Araneus gemmoides by Stauffer SL (1994)
10.1016/0092-8674(79)90018-7
10.1016/0092-8674(79)90075-8
10.1038/scientificamerican0392-70
10.1039/tf9565200554
10.1016/S0079-6603(08)60858-2
10.1073/pnas.87.18.7120

Dates

Type	When
Created	15 years, 1 month ago (July 12, 2010, 12:02 a.m.)
Deposited	1 year, 11 months ago (Aug. 29, 2023, 4:27 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 1:13 p.m.)
Issued	27 years, 5 months ago (March 1, 1998)
Published	27 years, 5 months ago (March 1, 1998)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	27 years, 5 months ago (March 1, 1998)

Funders 1

US. Army Research Office 10.13039/100000183 Army Research Office
Region: Americas
gov (National government)
Labels5
1. U.S. Army Research Office
2. United States Army Research Office
3. U.S. Army Research Laboratory's Army Research Office
4. ARL's Army Research Office
5. ARO
Awards3
1. AF207737
2. AF027735
3. AF027736

BibTeX

@article{Colgin_1998, title={Spider minor ampullate silk proteins contain new repetitive sequences and highly conserved non‐silk‐like “spacer regions”}, volume={7}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560070315}, DOI={10.1002/pro.5560070315}, number={3}, journal={Protein Science}, publisher={Wiley}, author={Colgin, Mark A. and Lewis, Randolph V.}, year={1998}, month=mar, pages={667–672} }

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