DOI: 10.1002/pro.5560061202. Identification of the binding site on S100B protein for the actin capping protein CapZ

Identification of the binding site on S100B protein for the actin capping protein CapZ

10.1002/pro.5560061202

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractThe calcium‐binding protein S100B binds to several potential target proteins, but there is no detailed information showing the location of the binding site for any target protein on S100B. We have made backbone assignments of the calcium‐bound form of S100B and used chemical‐shift changes in spectra of 15N‐labeled protein to locate the site that binds a peptide corresponding to residues 265‐276 from CapZα, the actin capping protein. The largest chemical‐shift changes are observed for resonances arising from residues around the C terminus of the C‐terminal helix of S100B and residues Val‐8 to Asp‐12 of the N‐terminal helix. These residues are close to but not identical to residues that have been identified by mutational analysis to be important in other S100 protein‐protein interactions. They make up a patch across the S100B dimer interface and include some residues that are quite buried in the structure of calcium‐free S100B. We believe we may have identified a binding site that could be common to many S100 protein—protein interactions.

Bibliography

Kilby, P. M., Roberts, G. C. K., & Van Eldik, L. J. (1997). Identification of the binding site on S100B protein for the actin capping protein CapZ. Protein Science, 6(12), 2494â2503. Portico.

Authors 3

Peter M. Kilby (first)
GORDON C. K. Roberts (additional)
Linda J. Van Eldik (additional)

References 37 Referenced 32

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Dates

Type	When
Created	16 years, 6 months ago (Feb. 10, 2009, 12:21 a.m.)
Deposited	1 year, 10 months ago (Oct. 28, 2023, 5:03 a.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 2:32 p.m.)
Issued	27 years, 9 months ago (Dec. 1, 1997)
Published	27 years, 9 months ago (Dec. 1, 1997)
Published Online	16 years, 8 months ago (Dec. 31, 2008)
Published Print	27 years, 9 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{Kilby_1997, title={Identification of the binding site on S100B protein for the actin capping protein CapZ}, volume={6}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560061202}, DOI={10.1002/pro.5560061202}, number={12}, journal={Protein Science}, publisher={Wiley}, author={Kilby, Peter M. and Roberts, GORDON C. K. and Van Eldik, Linda J.}, year={1997}, month=dec, pages={2494–2503} }

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