DOI: 10.1002/pro.5560060627. How protein chemists learned about the hydrophobic factor

How protein chemists learned about the hydrophobic factor

10.1002/pro.5560060627

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractIt is generally accepted today that the hydrophobic force is the dominant energetic factor that leads to the folding of polypeptide chains into compact globular entities. This principle was first explicitly introduced to protein chemists in 1938 by Irving Langmuir, past master in the application of hydrophobicity to other problems, and was enthusiastically endorsed by J.D. Bernal. But both proposal and endorsement came in the course of a debate about a quite different structural principle, the so‐called “cyclol hypothesis” proposed by D. Wrinch, which soon proved to be theoretically and experimentally unsupportable. Being a more tangible idea, directly expressed in structural terms, the cyclol hypothesis received more attention than the hydrophobic principle and the latter never actually entered the mainstream of protein science until 1959, when it was thrust into the limelight in a lucid review by W. Kauzmann. A theoretical paper by H.S. Frank and M. Evans, not itself related to protein folding, probably played a major role in the acceptance of the hydrophobicity concept by protein chemists because it provided a crude but tangible picture of the origin of hydrophobicity per se in terms of water structure.

Bibliography

Tanford, C. (1997). How protein chemists learned about the hydrophobic factor. Protein Science, 6(6), 1358â1366. Portico.

Authors 1

Charles Tanford (first)

References 78 Referenced 140

{'key': 'e_1_2_1_2_1', 'first-page': '239', 'volume-title': 'Uneasy careers and intimate lives', 'author': 'Abir‐am PG', 'year': '1987'} / Uneasy careers and intimate lives by Abir‐am PG (1987)
10.1016/S0065-3233(08)60629-4
10.1084/jem.79.2.137
10.1016/S0021-9258(18)74540-7 / J Biol Chem / On the structure of proteins: Cattle hemoglobin, egg albumin, cattle fibrin, and gelatin by Bergmann M (1937)
10.1038/143074a0
{'key': 'e_1_2_1_7_1', 'first-page': '541', 'article-title': 'Structure of proteins', 'volume': '30', 'author': 'Bernal JD', 'year': '1939', 'journal-title': 'Proc Roy Inst Gt Britain'} / Proc Roy Inst Gt Britain / Structure of proteins by Bernal JD (1939)
10.1039/df9582500007
10.1038/141523a0
10.1063/1.1749327
10.1038/143073a0
{'key': 'e_1_2_1_12_1', 'first-page': '1', 'article-title': 'Protein Structure', 'volume': '1', 'author': 'Bull HB', 'year': '1941', 'journal-title': 'Adv Enzymol'} / Adv Enzymol / Protein Structure by Bull HB (1941)
10.1039/tf9373300229
10.1097/00000441-194306000-00035
10.1098/rspa.1938.0038
{'key': 'e_1_2_1_16_1', 'first-page': '17', 'article-title': 'John Desmond Bernal', 'volume': '26', 'author': 'Crowfoot‐Hodgkin DM', 'year': '1980', 'journal-title': 'Biographical Memoirs of Fellows of the Royal Society'} / Biographical Memoirs of Fellows of the Royal Society / John Desmond Bernal by Crowfoot‐Hodgkin DM (1980)
10.1101/SQB.1938.006.01.019
{'key': 'e_1_2_1_18_1', 'first-page': '40', 'volume': '170', 'author': 'Discussion on the protein molecules.', 'year': '1939', 'journal-title': 'Proc Roy Soc London'} / Proc Roy Soc London by Discussion on the protein molecules. (1939)
{'key': 'e_1_2_1_19_1', 'first-page': '318', 'volume-title': 'Proteins, amino acids and peptides as ions and dipolar ions', 'author': 'Edsall JT', 'year': '1943'} / Proteins, amino acids and peptides as ions and dipolar ions by Edsall JT (1943)
{'key': 'e_1_2_1_20_1', 'first-page': '371', 'article-title': 'Isidor Traube: Physical chemist, biochemist, colloid chemist and controversialist', 'volume': '129', 'author': 'Edsall JT', 'year': '1985', 'journal-title': 'Proc Am Phil Soc'} / Proc Am Phil Soc / Isidor Traube: Physical chemist, biochemist, colloid chemist and controversialist by Edsall JT (1985)
10.1002/pro.5560011114
10.1016/S0065-3233(08)60329-0
{'issue': '50', 'key': 'e_1_2_1_23_1', 'first-page': '22.', 'article-title': 'Citation classic: Free volume and entropy in condensed systems', 'volume': '23', 'author': 'Frank HS', 'year': '1983', 'journal-title': 'Current Contents. Physical, Chemical & Earth Sciences'} / Current Contents. Physical, Chemical & Earth Sciences / Citation classic: Free volume and entropy in condensed systems by Frank HS (1983)
10.1063/1.1723985
10.1084/jem.41.4.439
{'key': 'e_1_2_1_26_1', 'volume-title': 'Force of nature: The life of Linus Pauling', 'author': 'Hager T.', 'year': '1995'} / Force of nature: The life of Linus Pauling by Hager T. (1995)
{'key': 'e_1_2_1_27_1', 'volume-title': 'Aqueous solutions of paraffin‐chain salts', 'author': 'Hartley GS', 'year': '1936'} / Aqueous solutions of paraffin‐chain salts by Hartley GS (1936)
10.1085/jgp.36.1.39
10.1038/260564a0
10.1038/164411a0
{'key': 'e_1_2_1_31_1', 'volume-title': 'The eighth day of creation. Makers of the revolution in biology', 'author': 'Judson HF', 'year': '1979'} / The eighth day of creation. Makers of the revolution in biology by Judson HF (1979)
{'key': 'e_1_2_1_32_1', 'first-page': '70', 'volume-title': 'The mechanism of enzyme action', 'author': 'Kauzmann W.', 'year': '1954'} / The mechanism of enzyme action by Kauzmann W. (1954)
10.1016/S0065-3233(08)60608-7
10.1002/pro.5560020418
{'key': 'e_1_2_1_35_1', 'first-page': '925', 'volume-title': 'Physical chemistry', 'author': 'Laidler KJ', 'year': '1995'} / Physical chemistry by Laidler KJ (1995)
10.1021/ja02254a006
{'key': 'e_1_2_1_37_1', 'first-page': '569', 'article-title': 'Fundamental research and its human value. A paper given at 17th Congress of Applied Chemistry, Paris, Sept. 30, 1937', 'volume': '40', 'author': 'Langmuir I.', 'year': '1937', 'journal-title': 'GE Review'} / GE Review / Fundamental research and its human value. A paper given at 17th Congress of Applied Chemistry, Paris, Sept. 30, 1937 by Langmuir I. (1937)
10.1101/SQB.1938.006.01.018
{'key': 'e_1_2_1_39_1', 'first-page': '483', 'article-title': 'The properties and structure of protein films', 'volume': '30', 'author': 'Langmuir I.', 'year': '1938', 'journal-title': 'Proc Roy Inst Gt Britain'} / Proc Roy Inst Gt Britain / The properties and structure of protein films by Langmuir I. (1938)
10.1098/rspa.1939.0017
10.1126/science.85.2194.76-a
10.1038/143049a0
10.1021/ja01452a015
10.1002/bip.360240507
{'key': 'e_1_2_1_45_1', 'volume-title': 'Lane medical lectures', 'author': 'Linderstrøm‐Lang KU', 'year': '1952'} / Lane medical lectures by Linderstrøm‐Lang KU (1952)
10.1021/ja01448a010
10.1073/pnas.22.7.439
{'key': 'e_1_2_1_48_1', 'first-page': '64', 'article-title': 'Chemical criticism of the cyclol and frequency hypothesis of protein structure', 'volume': '170', 'author': 'Neuberger A.', 'year': '1939', 'journal-title': 'Proc Roy Soc London'} / Proc Roy Soc London / Chemical criticism of the cyclol and frequency hypothesis of protein structure by Neuberger A. (1939)
10.1021/cr60076a001
10.1021/cr60108a003
10.1038/142994a0
10.1021/j150433a002
{'key': 'e_1_2_1_53_1', 'first-page': '276', 'volume-title': 'The specificity of serological reactions', 'author': 'Pauling L.', 'year': '1945'} / The specificity of serological reactions by Pauling L. (1945)
10.1038/161707a0
10.1073/pnas.37.11.729
10.1073/pnas.37.4.205
10.1021/ja01876a065
{'key': 'e_1_2_1_58_1', 'first-page': '10', 'volume-title': 'The hydrogen bond', 'author': 'Pimentel GC', 'year': '1960'} / The hydrogen bond by Pimentel GC (1960)
10.1351/pac198961061097
10.1101/SQB.1938.006.01.001
10.1146/annurev.bb.22.060193.002121
{'key': 'e_1_2_1_62_1', 'volume-title': 'The quintessence of Irving Langmuir', 'author': 'Rosenfeld A.', 'year': '1966'} / The quintessence of Irving Langmuir by Rosenfeld A. (1966)
10.1021/ja01500a015
{'key': 'e_1_2_1_64_1', 'volume-title': 'The search', 'author': 'Snow CP', 'year': '1934'} / The search by Snow CP (1934)
10.1007/BF01474473
{'key': 'e_1_2_1_66_1', 'first-page': '406', 'volume-title': 'The ultracentrifuge', 'author': 'Svedberg T', 'year': '1940'} / The ultracentrifuge by Svedberg T (1940)
{'key': 'e_1_2_1_67_1', 'first-page': '35', 'volume-title': 'Symposium on protein structure', 'author': 'Tanford C.', 'year': '1957'} / Symposium on protein structure by Tanford C. (1957)
{'key': 'e_1_2_1_68_1', 'volume-title': 'Ben Franklin stilled the waves', 'author': 'Tanford C.', 'year': '1989'} / Ben Franklin stilled the waves by Tanford C. (1989)
10.1021/ja01629a001
10.1002/jlac.18912650103
10.1016/S0065-3233(08)60210-7
10.1038/137411a0
10.1038/138241a0
10.1098/rspa.1937.0095
10.1098/rspa.1937.0159
10.1039/tf9373301368
10.1080/14786443808562056
{'key': 'e_1_2_1_78_1', 'first-page': '321', 'article-title': 'Studies on denaturation of proteins XIII. A theory of denaturation', 'volume': '5', 'author': 'Wu H.', 'year': '1931', 'journal-title': 'Chinese J Physiol'} / Chinese J Physiol / Studies on denaturation of proteins XIII. A theory of denaturation by Wu H. (1931)
10.1016/S0065-3233(08)60330-7

Dates

Type	When
Created	16 years, 6 months ago (Feb. 10, 2009, 12:37 a.m.)
Deposited	1 year, 9 months ago (Oct. 27, 2023, 4:48 a.m.)
Indexed	2 months, 3 weeks ago (May 29, 2025, 3:45 a.m.)
Issued	28 years, 2 months ago (June 1, 1997)
Published	28 years, 2 months ago (June 1, 1997)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	28 years, 2 months ago (June 1, 1997)

Funders 0

None

BibTeX

@article{Tanford_1997, title={How protein chemists learned about the hydrophobic factor}, volume={6}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560060627}, DOI={10.1002/pro.5560060627}, number={6}, journal={Protein Science}, publisher={Wiley}, author={Tanford, Charles}, year={1997}, month=jun, pages={1358–1366} }

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