DOI: 10.1002/pro.5560051008. Cation selective promotion of tubulin polymerization by alkali metal chlorides

Cation selective promotion of tubulin polymerization by alkali metal chlorides

10.1002/pro.5560051008

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractA role for charge‐based interactions in protein stability at the monomer or dimer level is well known. We show here that such interactions can also be important for the higher‐order structures of microtubule assembly. Alkali metal chlorides increase the rate of polymerization of pure tubulin driven by either taxol or dimethyl sulfoxide. The effect is cation selective, exhibiting a sequence Na+> K+> Li+> Cs+, with optimal concentrations for Na+at ∽ 160 mM. Hofmeister anion effects are additive with these rate stimulations. Sodium is less potent than guanidinium ion stimulation reported previously, but produces a larger fraction of normal microtubules. Alkali metal cations lower the critical concentration by a factor of ∽2, produce cold reversible polymers whose formation is sensitive to podophyllotoxin inhibition, increase the fraction of polymers present as microtubules from ∽0.9 to 0.99, and reverse or prevent urea‐induced depolymerization of microtubules. In the presence of microtubule‐associated proteins, the promotion of polymerization is no longer cation selective. In the polymerization of tubulin S, in which the acidic C termini of both monomers have been cleaved, the cation enhancement is markedly decreased, although selectivity persists.Because the selectivity sequence is similar to that of the coil/helix transition of polyglutamic acid, we suggest that a major part, although not all, of the cation selective enhancement of polymerization results from shielding of the glutamate‐rich C termini of the tubulin monomers.

Bibliography

Wolff, J., Sackett, D. L., & Knipling, L. (1996). Cation selective promotion of tubulin polymerization by alkali metal chlorides. Protein Science, 5(10), 2020â2028. Portico.

Authors 3

J. Wolff (first)
Dan L. Sackett (additional)
Leslie Knipling (additional)

References 58 Referenced 59

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Dates

Type	When
Created	16 years, 6 months ago (Feb. 9, 2009, 10:28 p.m.)
Deposited	6 months, 2 weeks ago (Feb. 7, 2025, 10:19 a.m.)
Indexed	6 months, 2 weeks ago (Feb. 8, 2025, 12:23 a.m.)
Issued	28 years, 10 months ago (Oct. 1, 1996)
Published	28 years, 10 months ago (Oct. 1, 1996)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	28 years, 10 months ago (Oct. 1, 1996)

Funders 0

None

BibTeX

@article{Wolff_1996, title={Cation selective promotion of tubulin polymerization by alkali metal chlorides}, volume={5}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560051008}, DOI={10.1002/pro.5560051008}, number={10}, journal={Protein Science}, publisher={Wiley}, author={Wolff, J. and Sackett, Dan L. and Knipling, Leslie}, year={1996}, month=oct, pages={2020–2028} }

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      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/pro.5560051008"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1996,
        10
      ]
    ]
  },
  "references-count": 58,
  "journal-issue": {
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    "published-print": {
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          1996,
          10
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      ]
    }
  },
  "alternative-id": [
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  "URL": "http://dx.doi.org/10.1002/pro.5560051008",
  "relation": {},
  "ISSN": [
    "0961-8368",
    "1469-896X"
  ],
  "subject": [],
  "container-title-short": "Protein Science",
  "published": {
    "date-parts": [
      [
        1996,
        10
      ]
    ]
  }
}