DOI: 10.1002/pro.5560030302. Structures of larger proteins, protein‐ligand and protein‐DNA complexes by multidimensional heteronuclear NMR

Structures of larger proteins, protein‐ligand and protein‐DNA complexes by multidimensional heteronuclear NMR

10.1002/pro.5560030302

Crossref journal-article

Wiley

Protein Science (311)

Abstract

AbstractThe recent development of a whole panoply of multidimensional heteronuclear‐edited and ‐filtered NMR experiments has revolutionized the field of protein structure determination by NMR, making it possible to extend the methodology from the IO‐kDa limit of conventional 2‐dimensional NMR to systems up to potentially 35‐40 kDa. The basic strategy for solving 3‐dimensional structures of larger proteins and protein‐ligand complexes in solution using 3‐ and 4‐dimensional NMR spectroscopy is summarized, and the power of these methods is illustrated using 3 examples: interleukin‐lβ, the complex of calmodulin with a target peptide, and the specific complex of the transcription factor GATA‐1 with its cognate DNA target site.

Bibliography

Clore, G. M., & Gronenborn, A. M. (1994). Structures of larger proteins, proteinâligand and proteinâDNA complexes by multidimensional heteronuclear NMR. Protein Science, 3(3), 372â390. Portico.

Authors 2

G. Marius Clore (first)
Angela M. Gronenborn (additional)

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Dates

Type	When
Created	15 years, 1 month ago (July 12, 2010, 1:21 a.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 8:06 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:47 p.m.)
Issued	31 years, 5 months ago (March 1, 1994)
Published	31 years, 5 months ago (March 1, 1994)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	31 years, 5 months ago (March 1, 1994)

Funders 1

DuPont Merck Pharmaceutical Company

BibTeX

@article{Clore_1994, title={Structures of larger proteins, protein‐ligand and protein‐DNA complexes by multidimensional heteronuclear NMR}, volume={3}, ISSN={1469-896X}, url={http://dx.doi.org/10.1002/pro.5560030302}, DOI={10.1002/pro.5560030302}, number={3}, journal={Protein Science}, publisher={Wiley}, author={Clore, G. Marius and Gronenborn, Angela M.}, year={1994}, month=mar, pages={372–390} }

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